Identification of natural resistance mediated by recognition of Phytophthora infestans effector gene Avr3aEM in potato
Late blight is considered the most renowned devastating potato disease worldwide. Resistance gene (R)-based resistance to late blight is the most effective method to inhibit infection by the causal agent Phytophthora infestans. However, the limited availability of resistant potato varieties and the...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2020
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/109106 |
| _version_ | 1855530580609859584 |
|---|---|
| author | Elnahal, A. Li, J. Wang, X. Zhou, C. Wang, J. Lindqvist-Kreuze, H. Meng, Y. Shan, W. |
| author_browse | Elnahal, A. Li, J. Lindqvist-Kreuze, H. Meng, Y. Shan, W. Wang, J. Wang, X. Zhou, C. |
| author_facet | Elnahal, A. Li, J. Wang, X. Zhou, C. Wang, J. Lindqvist-Kreuze, H. Meng, Y. Shan, W. |
| author_sort | Elnahal, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Late blight is considered the most renowned devastating potato disease worldwide. Resistance gene (R)-based resistance to late blight is the most effective method to inhibit infection by the causal agent Phytophthora infestans. However, the limited availability of resistant potato varieties and the rapid loss of R resistance, caused by P. infestans virulence variability, make disease control rely on fungicide application. We employed an Agrobacterium tumefaciens-mediated transient gene expression assay and effector biology approach to understand late blight resistance of Chinese varieties that showed years of promising field performance. We are particularly interested in PiAvr3aEM, the most common virulent allele of PiAvr3aKI that triggers a R3a-mediated hypersensitive response (HR) and late blight resistance. Through our significantly improved A. tumefaciens-mediated transient gene expression assay in potato using cultured seedlings, we characterized two dominant potato varieties, Qingshu9 and Longshu7, in China by transient expression of P. infestans effector genes. Transient expression of 10 known avirulence genes showed that PiAvr4 and PiAvr8 (PiAvrsmira2) could induce HR in Qingshu9, and PiAvrvnt1.1 in Longshu7, respectively. Our study also indicated that PiAvr3aEM is recognized by these two potato varieties, and is likely involved in their significant field performance of late blight resistance. The identification of natural resistance mediated by PiAvr3aEM recognition in Qingshu9 and Longshu7 will facilitate breeding for improved potato resistance against P. infestans. |
| format | Journal Article |
| id | CGSpace109106 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1091062025-11-29T05:22:18Z Identification of natural resistance mediated by recognition of Phytophthora infestans effector gene Avr3aEM in potato Elnahal, A. Li, J. Wang, X. Zhou, C. Wang, J. Lindqvist-Kreuze, H. Meng, Y. Shan, W. potatoes phytophthora infestans agrobacterium tumefaciens hypersensitivity Late blight is considered the most renowned devastating potato disease worldwide. Resistance gene (R)-based resistance to late blight is the most effective method to inhibit infection by the causal agent Phytophthora infestans. However, the limited availability of resistant potato varieties and the rapid loss of R resistance, caused by P. infestans virulence variability, make disease control rely on fungicide application. We employed an Agrobacterium tumefaciens-mediated transient gene expression assay and effector biology approach to understand late blight resistance of Chinese varieties that showed years of promising field performance. We are particularly interested in PiAvr3aEM, the most common virulent allele of PiAvr3aKI that triggers a R3a-mediated hypersensitive response (HR) and late blight resistance. Through our significantly improved A. tumefaciens-mediated transient gene expression assay in potato using cultured seedlings, we characterized two dominant potato varieties, Qingshu9 and Longshu7, in China by transient expression of P. infestans effector genes. Transient expression of 10 known avirulence genes showed that PiAvr4 and PiAvr8 (PiAvrsmira2) could induce HR in Qingshu9, and PiAvrvnt1.1 in Longshu7, respectively. Our study also indicated that PiAvr3aEM is recognized by these two potato varieties, and is likely involved in their significant field performance of late blight resistance. The identification of natural resistance mediated by PiAvr3aEM recognition in Qingshu9 and Longshu7 will facilitate breeding for improved potato resistance against P. infestans. 2020-06-19 2020-08-28T23:51:14Z 2020-08-28T23:51:14Z Journal Article https://hdl.handle.net/10568/109106 en Open Access Frontiers Media Elnahal, A.; Li, J.; Wang, X.; Zhou, C.; Wen, G.; Wang, J.; Lindqvist-Kreuze, H.; Meng, Y.; Shan, W. (2020). Identification of natural resistance mediated by recognition of Phytophthora infestans effector gene Avr3aEM in potato. Frontiers in Plant Science. Published online 19Jun2020. |
| spellingShingle | potatoes phytophthora infestans agrobacterium tumefaciens hypersensitivity Elnahal, A. Li, J. Wang, X. Zhou, C. Wang, J. Lindqvist-Kreuze, H. Meng, Y. Shan, W. Identification of natural resistance mediated by recognition of Phytophthora infestans effector gene Avr3aEM in potato |
| title | Identification of natural resistance mediated by recognition of Phytophthora infestans effector gene Avr3aEM in potato |
| title_full | Identification of natural resistance mediated by recognition of Phytophthora infestans effector gene Avr3aEM in potato |
| title_fullStr | Identification of natural resistance mediated by recognition of Phytophthora infestans effector gene Avr3aEM in potato |
| title_full_unstemmed | Identification of natural resistance mediated by recognition of Phytophthora infestans effector gene Avr3aEM in potato |
| title_short | Identification of natural resistance mediated by recognition of Phytophthora infestans effector gene Avr3aEM in potato |
| title_sort | identification of natural resistance mediated by recognition of phytophthora infestans effector gene avr3aem in potato |
| topic | potatoes phytophthora infestans agrobacterium tumefaciens hypersensitivity |
| url | https://hdl.handle.net/10568/109106 |
| work_keys_str_mv | AT elnahala identificationofnaturalresistancemediatedbyrecognitionofphytophthorainfestanseffectorgeneavr3aeminpotato AT lij identificationofnaturalresistancemediatedbyrecognitionofphytophthorainfestanseffectorgeneavr3aeminpotato AT wangx identificationofnaturalresistancemediatedbyrecognitionofphytophthorainfestanseffectorgeneavr3aeminpotato AT zhouc identificationofnaturalresistancemediatedbyrecognitionofphytophthorainfestanseffectorgeneavr3aeminpotato AT wangj identificationofnaturalresistancemediatedbyrecognitionofphytophthorainfestanseffectorgeneavr3aeminpotato AT lindqvistkreuzeh identificationofnaturalresistancemediatedbyrecognitionofphytophthorainfestanseffectorgeneavr3aeminpotato AT mengy identificationofnaturalresistancemediatedbyrecognitionofphytophthorainfestanseffectorgeneavr3aeminpotato AT shanw identificationofnaturalresistancemediatedbyrecognitionofphytophthorainfestanseffectorgeneavr3aeminpotato |