Functional evolution of the colony‐stimulating factor 1 receptor (CSF1R) and its ligands in birds
Macrophage colony-stimulating factor (CSF1 or M-CSF) and interleukin 34 (IL34) are secreted cytokines that control macrophage survival and differentiation. Both act through the CSF1 receptor (CSF1R), a type III transmembrane receptor tyrosine kinase. The functions of CSF1R and both ligands are conse...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Oxford University Press
2020
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| Acceso en línea: | https://hdl.handle.net/10568/109044 |
| _version_ | 1855518013773578240 |
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| author | Hume, D.A. Gutowska‐Ding, M.W. Garcia‐Morales, C. Kebede, A. Bamidele, Oladeji Trujillo, A.V. Gheyas, Almas A. Smith, J. |
| author_browse | Bamidele, Oladeji Garcia‐Morales, C. Gheyas, Almas A. Gutowska‐Ding, M.W. Hume, D.A. Kebede, A. Smith, J. Trujillo, A.V. |
| author_facet | Hume, D.A. Gutowska‐Ding, M.W. Garcia‐Morales, C. Kebede, A. Bamidele, Oladeji Trujillo, A.V. Gheyas, Almas A. Smith, J. |
| author_sort | Hume, D.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Macrophage colony-stimulating factor (CSF1 or M-CSF) and interleukin 34 (IL34) are secreted cytokines that control macrophage survival and differentiation. Both act through the CSF1 receptor (CSF1R), a type III transmembrane receptor tyrosine kinase. The functions of CSF1R and both ligands are conserved in birds. We have analyzed protein-coding sequence divergence among avian species. The intracellular tyrosine kinase domain of CSF1R was highly conserved in bird species as in mammals but the extracellular domain of avian CSF1R was more divergent in birds with multiple positively selected amino acids. Based upon crystal structures of the mammalian CSF1/IL34 receptor-ligand interfaces and structure-based alignments, we identified amino acids involved in avian receptor-ligand interactions. The contact amino acids in both CSF1 and CSF1R diverged among avian species. Ligand-binding domain swaps between chicken and zebra finch CSF1 confirmed the function of variants that confer species specificity on the interaction of CSF1 with CSF1R. Based upon genomic sequence analysis, we identified prevalent amino acid changes in the extracellular domain of CSF1R even within the chicken species that distinguished commercial broilers and layers and tropically adapted breeds. The rapid evolution in the extracellular domain of avian CSF1R suggests that at least in birds this ligand-receptor interaction is subjected to pathogen selection. We discuss this finding in the context of expression of CSF1R in antigen-sampling and antigen-presenting cells. |
| format | Journal Article |
| id | CGSpace109044 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1090442025-12-08T09:54:28Z Functional evolution of the colony‐stimulating factor 1 receptor (CSF1R) and its ligands in birds Hume, D.A. Gutowska‐Ding, M.W. Garcia‐Morales, C. Kebede, A. Bamidele, Oladeji Trujillo, A.V. Gheyas, Almas A. Smith, J. birds genetics animal breeding cell biology immunology Macrophage colony-stimulating factor (CSF1 or M-CSF) and interleukin 34 (IL34) are secreted cytokines that control macrophage survival and differentiation. Both act through the CSF1 receptor (CSF1R), a type III transmembrane receptor tyrosine kinase. The functions of CSF1R and both ligands are conserved in birds. We have analyzed protein-coding sequence divergence among avian species. The intracellular tyrosine kinase domain of CSF1R was highly conserved in bird species as in mammals but the extracellular domain of avian CSF1R was more divergent in birds with multiple positively selected amino acids. Based upon crystal structures of the mammalian CSF1/IL34 receptor-ligand interfaces and structure-based alignments, we identified amino acids involved in avian receptor-ligand interactions. The contact amino acids in both CSF1 and CSF1R diverged among avian species. Ligand-binding domain swaps between chicken and zebra finch CSF1 confirmed the function of variants that confer species specificity on the interaction of CSF1 with CSF1R. Based upon genomic sequence analysis, we identified prevalent amino acid changes in the extracellular domain of CSF1R even within the chicken species that distinguished commercial broilers and layers and tropically adapted breeds. The rapid evolution in the extracellular domain of avian CSF1R suggests that at least in birds this ligand-receptor interaction is subjected to pathogen selection. We discuss this finding in the context of expression of CSF1R in antigen-sampling and antigen-presenting cells. 2020-02-01 2020-08-20T19:09:55Z 2020-08-20T19:09:55Z Journal Article https://hdl.handle.net/10568/109044 en Open Access Oxford University Press Hume, D.A., Gutowska‐Ding, M.W., Garcia‐Morales, C., Kebede, A., Bamidele, O., Trujillo, A.V., Gheyas, A.A. and Smith, J. 2020. Functional evolution of the colony‐stimulating factor 1 receptor (CSF1R) and its ligands in birds. Journal of Leukocyte Biology 107(2):237-250. |
| spellingShingle | birds genetics animal breeding cell biology immunology Hume, D.A. Gutowska‐Ding, M.W. Garcia‐Morales, C. Kebede, A. Bamidele, Oladeji Trujillo, A.V. Gheyas, Almas A. Smith, J. Functional evolution of the colony‐stimulating factor 1 receptor (CSF1R) and its ligands in birds |
| title | Functional evolution of the colony‐stimulating factor 1 receptor (CSF1R) and its ligands in birds |
| title_full | Functional evolution of the colony‐stimulating factor 1 receptor (CSF1R) and its ligands in birds |
| title_fullStr | Functional evolution of the colony‐stimulating factor 1 receptor (CSF1R) and its ligands in birds |
| title_full_unstemmed | Functional evolution of the colony‐stimulating factor 1 receptor (CSF1R) and its ligands in birds |
| title_short | Functional evolution of the colony‐stimulating factor 1 receptor (CSF1R) and its ligands in birds |
| title_sort | functional evolution of the colony stimulating factor 1 receptor csf1r and its ligands in birds |
| topic | birds genetics animal breeding cell biology immunology |
| url | https://hdl.handle.net/10568/109044 |
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