The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa
Banana is a staple food and cash crop grown in East and Central Africa (ECA). The main banana varieties grown in ECA are the East African highland bananas (EAHB), although dessert/beer bananas such as Sukari Ndizi, Kayinja (Pisang Awak) and Gros Michel are also produced due to their high value at lo...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/109000 |
| _version_ | 1855531769195921408 |
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| author | Ndayihanzamaso, Privat Karangwa, Patrick Mostert, Diane Mahuku, George S. Blomme, Guy Beed, Fenton D. Swennen, Rony L. Viljoen, Altus |
| author_browse | Beed, Fenton D. Blomme, Guy Karangwa, Patrick Mahuku, George S. Mostert, Diane Ndayihanzamaso, Privat Swennen, Rony L. Viljoen, Altus |
| author_facet | Ndayihanzamaso, Privat Karangwa, Patrick Mostert, Diane Mahuku, George S. Blomme, Guy Beed, Fenton D. Swennen, Rony L. Viljoen, Altus |
| author_sort | Ndayihanzamaso, Privat |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Banana is a staple food and cash crop grown in East and Central Africa (ECA). The main banana varieties grown in ECA are the East African highland bananas (EAHB), although dessert/beer bananas such as Sukari Ndizi, Kayinja (Pisang Awak) and Gros Michel are also produced due to their high value at local markets. The Fusarium wilt fungus Fusarium oxysporum f. sp. cubense (Foc) causes disease of susceptible dessert/beer bananas, which significantly reduces yields. Banana Fusarium wilt is managed by excluding the pathogen from disease-free areas and by planting disease-resistant varieties in infested fields. Six phylogenetically closely-related vegetative compatibility groups (VCGs) of Foc, VCGs 0124, 0125, 0128, 01212, 01220 and 01222 are present in ECA, which all group together in Foc Lineage VI. Rapid and accurate detection of Foc Lineage VI strains is thus important to prevent its spread to disease-free areas. In this study, molecular markers specific to Foc Lineage VI were therefore developed. Primer sets were then combined in a multiplex PCR assay, and validated on a worldwide population of 623 known Foc isolates, other formae speciales and non-pathogenic Fusarium oxysporum isolates. The Foc Lineage VI multiplex PCR was used to identify Foc isolates collected in banana fields at five locations in Uganda and Tanzania. Foc Lineage VI DNA was detected at a concentration as low as 0.1 ng/μl, both in the absence and presence of banana DNA, and can therefore be used as an accurate diagnostic tool for Foc Lineage VI strains. |
| format | Journal Article |
| id | CGSpace109000 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1090002025-11-12T05:44:52Z The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa Ndayihanzamaso, Privat Karangwa, Patrick Mostert, Diane Mahuku, George S. Blomme, Guy Beed, Fenton D. Swennen, Rony L. Viljoen, Altus bananas panama disease disease control genetic markers enfermedad de panamá control de enfermedades marcadores genéticos Banana is a staple food and cash crop grown in East and Central Africa (ECA). The main banana varieties grown in ECA are the East African highland bananas (EAHB), although dessert/beer bananas such as Sukari Ndizi, Kayinja (Pisang Awak) and Gros Michel are also produced due to their high value at local markets. The Fusarium wilt fungus Fusarium oxysporum f. sp. cubense (Foc) causes disease of susceptible dessert/beer bananas, which significantly reduces yields. Banana Fusarium wilt is managed by excluding the pathogen from disease-free areas and by planting disease-resistant varieties in infested fields. Six phylogenetically closely-related vegetative compatibility groups (VCGs) of Foc, VCGs 0124, 0125, 0128, 01212, 01220 and 01222 are present in ECA, which all group together in Foc Lineage VI. Rapid and accurate detection of Foc Lineage VI strains is thus important to prevent its spread to disease-free areas. In this study, molecular markers specific to Foc Lineage VI were therefore developed. Primer sets were then combined in a multiplex PCR assay, and validated on a worldwide population of 623 known Foc isolates, other formae speciales and non-pathogenic Fusarium oxysporum isolates. The Foc Lineage VI multiplex PCR was used to identify Foc isolates collected in banana fields at five locations in Uganda and Tanzania. Foc Lineage VI DNA was detected at a concentration as low as 0.1 ng/μl, both in the absence and presence of banana DNA, and can therefore be used as an accurate diagnostic tool for Foc Lineage VI strains. 2020-10 2020-08-12T07:21:37Z 2020-08-12T07:21:37Z Journal Article https://hdl.handle.net/10568/109000 en Open Access application/pdf Springer Ndayihanzamaso, P.; Karangwa, P.; Mostert, D.; Mahuku, G.; Blomme, G.; Beed, F.; Swennen, R.; Viljoen, A. (2020) The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa. European Journal of Plant Pathology, Online first paper (02 August 2020). ISSN: 0929-1873 |
| spellingShingle | bananas panama disease disease control genetic markers enfermedad de panamá control de enfermedades marcadores genéticos Ndayihanzamaso, Privat Karangwa, Patrick Mostert, Diane Mahuku, George S. Blomme, Guy Beed, Fenton D. Swennen, Rony L. Viljoen, Altus The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa |
| title | The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa |
| title_full | The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa |
| title_fullStr | The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa |
| title_full_unstemmed | The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa |
| title_short | The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa |
| title_sort | development of a multiplex pcr assay for the detection of fusarium oxysporum f sp cubense lineage vi strains in east and central africa |
| topic | bananas panama disease disease control genetic markers enfermedad de panamá control de enfermedades marcadores genéticos |
| url | https://hdl.handle.net/10568/109000 |
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