Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines
The number of drought and low‐N tolerant hybrids with elevated levels of provitamin A (PVA) in sub‐Saharan Africa could increase when PVA genes are optimized and validated for developed drought and low‐N tolerant inbred lines. This study aimed to (a) determine the levels of drought and low‐N toleran...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Wiley
2020
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| Online Access: | https://hdl.handle.net/10568/108858 |
| _version_ | 1855513912187813888 |
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| author | Obeng‐Bio, E. Badu-Apraku, Baffour Ifie, B.E. Danquah, A. Blay, E.T. Dadzie, M.A. |
| author_browse | Badu-Apraku, Baffour Blay, E.T. Dadzie, M.A. Danquah, A. Ifie, B.E. Obeng‐Bio, E. |
| author_facet | Obeng‐Bio, E. Badu-Apraku, Baffour Ifie, B.E. Danquah, A. Blay, E.T. Dadzie, M.A. |
| author_sort | Obeng‐Bio, E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The number of drought and low‐N tolerant hybrids with elevated levels of provitamin A (PVA) in sub‐Saharan Africa could increase when PVA genes are optimized and validated for developed drought and low‐N tolerant inbred lines. This study aimed to (a) determine the levels of drought and low‐N tolerance, and PVA concentrations in early maturing PVA‐quality protein maize (QPM) inbred lines, and (b) identify lines harbouring the crtRB1 and LcyE genes as sources of favourable alleles of PVA. Seventy early maturing PVA‐QPM inbreds were evaluated under drought, low‐N and optimal environments in Nigeria for two years. The inbreds were assayed for PVA levels and the presence of PVA genes using allele‐specific PCR markers. Moderate range of PVA contents was observed for the inbreds. Nonetheless, TZEIORQ 55 combined high PVA concentration with drought and low‐N tolerance. The crtRB1‐3′TE primer and the KASP SNP (snpZM0015) consistently identified nine inbreds including TZEIORQ 55 harbouring the favourable alleles of the crtRB1 gene. These inbreds could serve as donor parents of the favourable crtRB1‐3′TE allele for PVA breeding in maize. |
| format | Journal Article |
| id | CGSpace108858 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1088582025-11-11T10:01:21Z Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines Obeng‐Bio, E. Badu-Apraku, Baffour Ifie, B.E. Danquah, A. Blay, E.T. Dadzie, M.A. genes maize maturity proteins inbred lines provitamins drought stress genetics The number of drought and low‐N tolerant hybrids with elevated levels of provitamin A (PVA) in sub‐Saharan Africa could increase when PVA genes are optimized and validated for developed drought and low‐N tolerant inbred lines. This study aimed to (a) determine the levels of drought and low‐N tolerance, and PVA concentrations in early maturing PVA‐quality protein maize (QPM) inbred lines, and (b) identify lines harbouring the crtRB1 and LcyE genes as sources of favourable alleles of PVA. Seventy early maturing PVA‐QPM inbreds were evaluated under drought, low‐N and optimal environments in Nigeria for two years. The inbreds were assayed for PVA levels and the presence of PVA genes using allele‐specific PCR markers. Moderate range of PVA contents was observed for the inbreds. Nonetheless, TZEIORQ 55 combined high PVA concentration with drought and low‐N tolerance. The crtRB1‐3′TE primer and the KASP SNP (snpZM0015) consistently identified nine inbreds including TZEIORQ 55 harbouring the favourable alleles of the crtRB1 gene. These inbreds could serve as donor parents of the favourable crtRB1‐3′TE allele for PVA breeding in maize. 2020-06 2020-07-23T13:55:50Z 2020-07-23T13:55:50Z Journal Article https://hdl.handle.net/10568/108858 en Open Access application/pdf Wiley Obeng‐Bio, E., Badu‐Apraku, B., Ifie, B.E., Danquah, A., Blay, E.T. & Dadzie, M.A. (2019). Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A‐quality protein maize (Zea mays) inbred lines. Plant Breeding, 1-14. |
| spellingShingle | genes maize maturity proteins inbred lines provitamins drought stress genetics Obeng‐Bio, E. Badu-Apraku, Baffour Ifie, B.E. Danquah, A. Blay, E.T. Dadzie, M.A. Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines |
| title | Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines |
| title_full | Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines |
| title_fullStr | Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines |
| title_full_unstemmed | Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines |
| title_short | Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines |
| title_sort | phenotypic characterization and validation of provitamin a functional genes in early maturing provitamin a quality protein maize zea mays inbred lines |
| topic | genes maize maturity proteins inbred lines provitamins drought stress genetics |
| url | https://hdl.handle.net/10568/108858 |
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