Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa

The effectiveness of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin isolates Bb11, Bb115, Bb116 and Bb362 against the diamondback moth (DBM), Plutella xylostella (L.) population from Cotonou, Benin, was investigated in the laboratory and on station. In the laboratory, six concent...

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Autores principales: Agboyi, L.K., Ketoh, G.K., Kpindou, O.K.D., Martin, T., Glitho, I.A., Tamò, Manuele
Formato: Journal Article
Lenguaje:Inglés
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://hdl.handle.net/10568/108842
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author Agboyi, L.K.
Ketoh, G.K.
Kpindou, O.K.D.
Martin, T.
Glitho, I.A.
Tamò, Manuele
author_browse Agboyi, L.K.
Glitho, I.A.
Ketoh, G.K.
Kpindou, O.K.D.
Martin, T.
Tamò, Manuele
author_facet Agboyi, L.K.
Ketoh, G.K.
Kpindou, O.K.D.
Martin, T.
Glitho, I.A.
Tamò, Manuele
author_sort Agboyi, L.K.
collection Repository of Agricultural Research Outputs (CGSpace)
description The effectiveness of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin isolates Bb11, Bb115, Bb116 and Bb362 against the diamondback moth (DBM), Plutella xylostella (L.) population from Cotonou, Benin, was investigated in the laboratory and on station. In the laboratory, six concentrations, 104, 105, 106, 107, 108 and 109 conidia/ml of each isolate were used to assess B. bassiana virulence. Third instar DBM larvae were inoculated with 2 µl of each isolate’s concentrations by topical application method. Control larvae were inoculated with sterilized Tween 80 solution (0.05%). Compared with other isolates, Bb11 was more virulent against DBM with LD50 value estimated to 4.96 × 106 conidia/ml and the highest rates of cadaver sporulation. The effectiveness of Bb11 against DBM on cabbage was subsequently assessed on station and on farm in Benin and Togo, at a low doses of 53 g/ha once or twice a week and a high dose of 315 g/ha weekly. Compared with unsprayed control and deltamethrin treatments, the dose of 53 g/ha of Bb11 applied twice a week at intervals of 4 days was able to reduce on farm the density of DBM by 83% and 93%, respectively. Also, the marketable cabbage yield obtained on farm with this dose was significantly higher compared with the other treatments, with a 199% and 452% increase in Danyi and Cotonou over the unsprayed control, respectively. These results confirm the better performance of Bb11 over other isolates, and also indicate that the newly investigated application dosage and frequency of Bb11 could be an efficient management option to control DBM in Benin and Togo.
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spelling CGSpace1088422025-10-16T09:52:51Z Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa Agboyi, L.K. Ketoh, G.K. Kpindou, O.K.D. Martin, T. Glitho, I.A. Tamò, Manuele brassica oleracea plutella xylostella entomogenous fungi beauveria bassiana The effectiveness of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin isolates Bb11, Bb115, Bb116 and Bb362 against the diamondback moth (DBM), Plutella xylostella (L.) population from Cotonou, Benin, was investigated in the laboratory and on station. In the laboratory, six concentrations, 104, 105, 106, 107, 108 and 109 conidia/ml of each isolate were used to assess B. bassiana virulence. Third instar DBM larvae were inoculated with 2 µl of each isolate’s concentrations by topical application method. Control larvae were inoculated with sterilized Tween 80 solution (0.05%). Compared with other isolates, Bb11 was more virulent against DBM with LD50 value estimated to 4.96 × 106 conidia/ml and the highest rates of cadaver sporulation. The effectiveness of Bb11 against DBM on cabbage was subsequently assessed on station and on farm in Benin and Togo, at a low doses of 53 g/ha once or twice a week and a high dose of 315 g/ha weekly. Compared with unsprayed control and deltamethrin treatments, the dose of 53 g/ha of Bb11 applied twice a week at intervals of 4 days was able to reduce on farm the density of DBM by 83% and 93%, respectively. Also, the marketable cabbage yield obtained on farm with this dose was significantly higher compared with the other treatments, with a 199% and 452% increase in Danyi and Cotonou over the unsprayed control, respectively. These results confirm the better performance of Bb11 over other isolates, and also indicate that the newly investigated application dosage and frequency of Bb11 could be an efficient management option to control DBM in Benin and Togo. 2020-05 2020-07-22T11:23:37Z 2020-07-22T11:23:37Z Journal Article https://hdl.handle.net/10568/108842 en Limited Access Elsevier Agboyi, L.K., Ketoh, G.K., Kpindou, O.K.D., Martin, T., Glitho, I.A. & Tamὸ, M. (2020). Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa. Biological Control, 144, 1-9.
spellingShingle brassica oleracea
plutella xylostella
entomogenous fungi
beauveria bassiana
Agboyi, L.K.
Ketoh, G.K.
Kpindou, O.K.D.
Martin, T.
Glitho, I.A.
Tamò, Manuele
Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa
title Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa
title_full Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa
title_fullStr Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa
title_full_unstemmed Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa
title_short Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa
title_sort improving the efficiency of beauveria bassiana applications for sustainable management of plutella xylostella lepidoptera plutellidae in west africa
topic brassica oleracea
plutella xylostella
entomogenous fungi
beauveria bassiana
url https://hdl.handle.net/10568/108842
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