Walnut SSR raw data, Central Asia
Between September 2013 and September 2015, samples of Juglans regia L. were collected from three countries in Central Asia: Tajikistan, Uzbekistan, and Kyrgyzstan. We selected 360 sample individuals from wild tree population (four populations of 30 individuals in each country) and 270 samples from c...
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| Formato: | Conjunto de datos |
| Lenguaje: | Inglés |
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Bioversity International and the International Center for Tropical Agriculture
2020
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| Acceso en línea: | https://hdl.handle.net/10568/108544 |
| _version_ | 1855524901841010688 |
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| author | Gaisberger, Hannes Legay, Sylvain |
| author_browse | Gaisberger, Hannes Legay, Sylvain |
| author_facet | Gaisberger, Hannes Legay, Sylvain |
| author_sort | Gaisberger, Hannes |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Between September 2013 and September 2015, samples of Juglans regia L. were collected from three countries in Central Asia: Tajikistan, Uzbekistan, and Kyrgyzstan. We selected 360 sample individuals from wild tree population (four populations of 30 individuals in each country) and 270 samples from cultivated populations from home gardens (three populations of 30 individuals in each country). Within each wild population, a systematic sampling experimental design has been chosen to collect the 30 individual adult trees with at 50m regular intervals. From each sampled tree, three leaves were collected and stored in silica for subsequent genetic analyses conducted at the Luxembourg Institute of Science and Technology (LIST). Genomic DNA was extracted from ground silica dried leaf material using the NucleoSpin Plant II kit (Macherey-Nagel). All SSR loci were PCR amplified following Pollegioni et al. (2014) and multiplex PCR products analyzed on an ABI3500 Genetic Analyser (Life Technologies, Waltham, MA). Subsequently, fragment analysis was conducted using the Genemapper 5.0 software. ND: No Data after re-extraction and re-amplification of the sample. |
| format | Conjunto de datos |
| id | CGSpace108544 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Bioversity International and the International Center for Tropical Agriculture |
| publisherStr | Bioversity International and the International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace1085442025-12-08T10:29:22Z Walnut SSR raw data, Central Asia Gaisberger, Hannes Legay, Sylvain climate change cambio climatico genetics genetica trees arboles juglans regia Between September 2013 and September 2015, samples of Juglans regia L. were collected from three countries in Central Asia: Tajikistan, Uzbekistan, and Kyrgyzstan. We selected 360 sample individuals from wild tree population (four populations of 30 individuals in each country) and 270 samples from cultivated populations from home gardens (three populations of 30 individuals in each country). Within each wild population, a systematic sampling experimental design has been chosen to collect the 30 individual adult trees with at 50m regular intervals. From each sampled tree, three leaves were collected and stored in silica for subsequent genetic analyses conducted at the Luxembourg Institute of Science and Technology (LIST). Genomic DNA was extracted from ground silica dried leaf material using the NucleoSpin Plant II kit (Macherey-Nagel). All SSR loci were PCR amplified following Pollegioni et al. (2014) and multiplex PCR products analyzed on an ABI3500 Genetic Analyser (Life Technologies, Waltham, MA). Subsequently, fragment analysis was conducted using the Genemapper 5.0 software. ND: No Data after re-extraction and re-amplification of the sample. 2020-05 2020-06-22T02:26:14Z 2020-06-22T02:26:14Z Dataset https://hdl.handle.net/10568/108544 en https://hdl.handle.net/10568/108277 Open Access Bioversity International and the International Center for Tropical Agriculture Gaisberger, H.; Legay, S., 2020 Walnut SSR raw data, Central Asia, https://doi.org/10.7910/DVN/VUP7OH, Harvard Dataverse, V1, UNF:6:254awoJkmwuQGPFAPzG/Aw== [fileUNF] |
| spellingShingle | climate change cambio climatico genetics genetica trees arboles juglans regia Gaisberger, Hannes Legay, Sylvain Walnut SSR raw data, Central Asia |
| title | Walnut SSR raw data, Central Asia |
| title_full | Walnut SSR raw data, Central Asia |
| title_fullStr | Walnut SSR raw data, Central Asia |
| title_full_unstemmed | Walnut SSR raw data, Central Asia |
| title_short | Walnut SSR raw data, Central Asia |
| title_sort | walnut ssr raw data central asia |
| topic | climate change cambio climatico genetics genetica trees arboles juglans regia |
| url | https://hdl.handle.net/10568/108544 |
| work_keys_str_mv | AT gaisbergerhannes walnutssrrawdatacentralasia AT legaysylvain walnutssrrawdatacentralasia |