Genetic diversity of Ecuadorian cocoa from the germplasm bank of Tehìnguel-Guyas Ecuador based in SNPP'S
The genetic diversity and structure of 80 cocoa introductions of Theobroma cacao L. Nacional variety existing in the farm “La Buseta”, Tenguel-Guayas, Ecuador, was studied. The characterization included 19 controls and the use of 96 markers of simple nucleotide polymorphisms (SNPs), in the Fluidigm...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2020
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| Acceso en línea: | https://hdl.handle.net/10568/108280 |
| _version_ | 1855534424931696640 |
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| author | Carranza, Mercedes Susana Zapata, Yeny Patricia Gallego, Gerardo Rodríguez, José Nieto Morante Carriel, Jaime Cruz Rosero, Nicolás Medina Jara, Sila |
| author_browse | Carranza, Mercedes Susana Cruz Rosero, Nicolás Gallego, Gerardo Medina Jara, Sila Morante Carriel, Jaime Rodríguez, José Nieto Zapata, Yeny Patricia |
| author_facet | Carranza, Mercedes Susana Zapata, Yeny Patricia Gallego, Gerardo Rodríguez, José Nieto Morante Carriel, Jaime Cruz Rosero, Nicolás Medina Jara, Sila |
| author_sort | Carranza, Mercedes Susana |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The genetic diversity and structure of 80 cocoa introductions of Theobroma cacao L. Nacional variety existing in the farm “La Buseta”, Tenguel-Guayas, Ecuador, was studied. The characterization included 19 controls and the use of 96 markers of simple nucleotide polymorphisms (SNPs), in the Fluidigm EP1 system platform. The SNPs showed to be informative, with a mean content of polymorphic information of 0.289, and an average observed heterozygosity of 0.479 and expected heterozygosity of 0.378. The genetic profiles of the introductions were obtained from which 16 groups of identical introductions were identified. The identity probability analysis, including siblings (PIsib), concluded that the SNPs were enough to differentiate the introductions. The average of heterozygosity of the samples was 0.432 being higher in the group F (0.646) and lower in the group P (0.253). The Criollo and Contamana genotyping controls showed greater genetic distance than the Trinitario, Forastero and Ecuadorian Criollo controls. The maximum genetic distance between the introductions was 0.167. The results are consistent with the history of cultivation of the Nacional cacao, which over time received genetic contributions from other varieties. The present study constitutes a significant advance in the knowledge of the genetic diversity of Ecuador's Nacional cacao. |
| format | Journal Article |
| id | CGSpace108280 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| record_format | dspace |
| spelling | CGSpace1082802025-11-11T19:01:32Z Genetic diversity of Ecuadorian cocoa from the germplasm bank of Tehìnguel-Guyas Ecuador based in SNPP'S Carranza, Mercedes Susana Zapata, Yeny Patricia Gallego, Gerardo Rodríguez, José Nieto Morante Carriel, Jaime Cruz Rosero, Nicolás Medina Jara, Sila genetic variation germplasm genetic markers variatión genética germoplasma marcadores genéticos The genetic diversity and structure of 80 cocoa introductions of Theobroma cacao L. Nacional variety existing in the farm “La Buseta”, Tenguel-Guayas, Ecuador, was studied. The characterization included 19 controls and the use of 96 markers of simple nucleotide polymorphisms (SNPs), in the Fluidigm EP1 system platform. The SNPs showed to be informative, with a mean content of polymorphic information of 0.289, and an average observed heterozygosity of 0.479 and expected heterozygosity of 0.378. The genetic profiles of the introductions were obtained from which 16 groups of identical introductions were identified. The identity probability analysis, including siblings (PIsib), concluded that the SNPs were enough to differentiate the introductions. The average of heterozygosity of the samples was 0.432 being higher in the group F (0.646) and lower in the group P (0.253). The Criollo and Contamana genotyping controls showed greater genetic distance than the Trinitario, Forastero and Ecuadorian Criollo controls. The maximum genetic distance between the introductions was 0.167. The results are consistent with the history of cultivation of the Nacional cacao, which over time received genetic contributions from other varieties. The present study constitutes a significant advance in the knowledge of the genetic diversity of Ecuador's Nacional cacao. 2020-05 2020-05-19T17:27:38Z 2020-05-19T17:27:38Z Journal Article https://hdl.handle.net/10568/108280 en Open Access application/pdf Carranza, M.S.; Zapata, Y.P.; Gallego, G. ; Rodríguez, J.N.; Morante Carriel, J. ; Cruz Rosero, N.; Medina Jara, S. ; Muñoz, J.E. (2020) Genetic diversity of Ecuadorian cocoa from the germplasm bank of Tehìnguel-Guyas Ecuador based in SNPP'S. Bioagro 32(2) p. 75-86 ISSN: 1316-3361 |
| spellingShingle | genetic variation germplasm genetic markers variatión genética germoplasma marcadores genéticos Carranza, Mercedes Susana Zapata, Yeny Patricia Gallego, Gerardo Rodríguez, José Nieto Morante Carriel, Jaime Cruz Rosero, Nicolás Medina Jara, Sila Genetic diversity of Ecuadorian cocoa from the germplasm bank of Tehìnguel-Guyas Ecuador based in SNPP'S |
| title | Genetic diversity of Ecuadorian cocoa from the germplasm bank of Tehìnguel-Guyas Ecuador based in SNPP'S |
| title_full | Genetic diversity of Ecuadorian cocoa from the germplasm bank of Tehìnguel-Guyas Ecuador based in SNPP'S |
| title_fullStr | Genetic diversity of Ecuadorian cocoa from the germplasm bank of Tehìnguel-Guyas Ecuador based in SNPP'S |
| title_full_unstemmed | Genetic diversity of Ecuadorian cocoa from the germplasm bank of Tehìnguel-Guyas Ecuador based in SNPP'S |
| title_short | Genetic diversity of Ecuadorian cocoa from the germplasm bank of Tehìnguel-Guyas Ecuador based in SNPP'S |
| title_sort | genetic diversity of ecuadorian cocoa from the germplasm bank of tehinguel guyas ecuador based in snpp s |
| topic | genetic variation germplasm genetic markers variatión genética germoplasma marcadores genéticos |
| url | https://hdl.handle.net/10568/108280 |
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