Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya
Objective Animal African trypanosomiasis (AAT) is a life-threatening vector-borne disease, caused by trypanosome parasites, which are principally transmitted by tsetse flies. In Kenya, the prevalence of drug-resistant trypanosomes in endemic regions remains poorly understood. The objective of this s...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/108243 |
| _version_ | 1855538872742576128 |
|---|---|
| author | Kulohoma, B.W. Wamwenje, S.A.O. Wangwe, I.I. Masila, N. Mirieri, C.K. Wambua, Lillian |
| author_browse | Kulohoma, B.W. Masila, N. Mirieri, C.K. Wambua, Lillian Wamwenje, S.A.O. Wangwe, I.I. |
| author_facet | Kulohoma, B.W. Wamwenje, S.A.O. Wangwe, I.I. Masila, N. Mirieri, C.K. Wambua, Lillian |
| author_sort | Kulohoma, B.W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Objective Animal African trypanosomiasis (AAT) is a life-threatening vector-borne disease, caused by trypanosome parasites, which are principally transmitted by tsetse flies. In Kenya, the prevalence of drug-resistant trypanosomes in endemic regions remains poorly understood. The objective of this study was to establish AAT point prevalence, drug susceptibility of associated trypanosomes, and measure infectivity by multiple AAT mammalian hosts to tsetse flies in Shimba hills, a resource-poor region with high bovine trypanosomiasis prevalence and morbidity rates at the coast of Kenya. We collected tsetse flies using traps (1 Ngu and 2 biconical), and then sorted them on sex and species. Trypanosomes present in tsetse flies were detected by first extracting all genomic DNA, and then performing PCR reactions with established primers of the internal transcribed spacer regions. Polymorphisms associated with trypanocide resistance in the TbAT1 gene were also detected by performing PCR reactions with established primers. Results Our findings suggest low trypanosome prevalence (3.7%), low trypanocide resistance, and low infectivity by multiple mammalian hosts to tsetse flies in Shimba hills. We conclude that enhanced surveillance is crucial for informing disease management practices that help prevent the spread of drug-resistant trypanosomiasis. |
| format | Journal Article |
| id | CGSpace108243 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1082432025-03-11T12:14:31Z Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya Kulohoma, B.W. Wamwenje, S.A.O. Wangwe, I.I. Masila, N. Mirieri, C.K. Wambua, Lillian trypanosomiasis drug resistance animal diseases research Objective Animal African trypanosomiasis (AAT) is a life-threatening vector-borne disease, caused by trypanosome parasites, which are principally transmitted by tsetse flies. In Kenya, the prevalence of drug-resistant trypanosomes in endemic regions remains poorly understood. The objective of this study was to establish AAT point prevalence, drug susceptibility of associated trypanosomes, and measure infectivity by multiple AAT mammalian hosts to tsetse flies in Shimba hills, a resource-poor region with high bovine trypanosomiasis prevalence and morbidity rates at the coast of Kenya. We collected tsetse flies using traps (1 Ngu and 2 biconical), and then sorted them on sex and species. Trypanosomes present in tsetse flies were detected by first extracting all genomic DNA, and then performing PCR reactions with established primers of the internal transcribed spacer regions. Polymorphisms associated with trypanocide resistance in the TbAT1 gene were also detected by performing PCR reactions with established primers. Results Our findings suggest low trypanosome prevalence (3.7%), low trypanocide resistance, and low infectivity by multiple mammalian hosts to tsetse flies in Shimba hills. We conclude that enhanced surveillance is crucial for informing disease management practices that help prevent the spread of drug-resistant trypanosomiasis. 2020-12 2020-05-14T12:27:27Z 2020-05-14T12:27:27Z Journal Article https://hdl.handle.net/10568/108243 en Open Access Springer Kulohoma, B.W., Wamwenje, S.A.O., Wangwe, I.I., Masila, N., Mirieri, C.K. and Wambua, L. 2020. Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya. BMC Research Notes 13: 234. |
| spellingShingle | trypanosomiasis drug resistance animal diseases research Kulohoma, B.W. Wamwenje, S.A.O. Wangwe, I.I. Masila, N. Mirieri, C.K. Wambua, Lillian Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya |
| title | Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya |
| title_full | Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya |
| title_fullStr | Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya |
| title_full_unstemmed | Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya |
| title_short | Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya |
| title_sort | prevalence of trypanosomes associated with drug resistance in shimba hills kwale county kenya |
| topic | trypanosomiasis drug resistance animal diseases research |
| url | https://hdl.handle.net/10568/108243 |
| work_keys_str_mv | AT kulohomabw prevalenceoftrypanosomesassociatedwithdrugresistanceinshimbahillskwalecountykenya AT wamwenjesao prevalenceoftrypanosomesassociatedwithdrugresistanceinshimbahillskwalecountykenya AT wangweii prevalenceoftrypanosomesassociatedwithdrugresistanceinshimbahillskwalecountykenya AT masilan prevalenceoftrypanosomesassociatedwithdrugresistanceinshimbahillskwalecountykenya AT mirierick prevalenceoftrypanosomesassociatedwithdrugresistanceinshimbahillskwalecountykenya AT wambualillian prevalenceoftrypanosomesassociatedwithdrugresistanceinshimbahillskwalecountykenya |