Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya
Background Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serologica...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2020
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| Online Access: | https://hdl.handle.net/10568/108242 |
| _version_ | 1855537645598277632 |
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| author | Akoko, James M. Pelle, Roger Kivali, Velma Schelling, E. Shirima, G. Machuka, Eunice M. Mathew, C. Fèvre, Eric M. Kyallo, Victoria Falzon, Laura C. Lukambagire, A.S. Halliday, J.E.B. Bonfoh, Bassirou Kazwala, R. Ouma, C. |
| author_browse | Akoko, James M. Bonfoh, Bassirou Falzon, Laura C. Fèvre, Eric M. Halliday, J.E.B. Kazwala, R. Kivali, Velma Kyallo, Victoria Lukambagire, A.S. Machuka, Eunice M. Mathew, C. Ouma, C. Pelle, Roger Schelling, E. Shirima, G. |
| author_facet | Akoko, James M. Pelle, Roger Kivali, Velma Schelling, E. Shirima, G. Machuka, Eunice M. Mathew, C. Fèvre, Eric M. Kyallo, Victoria Falzon, Laura C. Lukambagire, A.S. Halliday, J.E.B. Bonfoh, Bassirou Kazwala, R. Ouma, C. |
| author_sort | Akoko, James M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RT-PCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs. |
| format | Journal Article |
| id | CGSpace108242 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1082422025-12-02T10:59:51Z Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya Akoko, James M. Pelle, Roger Kivali, Velma Schelling, E. Shirima, G. Machuka, Eunice M. Mathew, C. Fèvre, Eric M. Kyallo, Victoria Falzon, Laura C. Lukambagire, A.S. Halliday, J.E.B. Bonfoh, Bassirou Kazwala, R. Ouma, C. brucella swine serology Background Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RT-PCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs. 2020-12 2020-05-14T12:24:50Z 2020-05-14T12:24:50Z Journal Article https://hdl.handle.net/10568/108242 en Open Access Springer Akoko, J., Pelle, R., Kivali, V., Schelling, E., Shirima, G., Machuka, E.M., Mathew, C., Fèvre, E.M., Kyallo, V., Falzon, L.C., Lukambagire, A.S., Halliday, J.E.B., Bonfoh, B., Kazwala, R. and Ouma, C. 2020. Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya. BMC Veterinary Research 16: 133. |
| spellingShingle | brucella swine serology Akoko, James M. Pelle, Roger Kivali, Velma Schelling, E. Shirima, G. Machuka, Eunice M. Mathew, C. Fèvre, Eric M. Kyallo, Victoria Falzon, Laura C. Lukambagire, A.S. Halliday, J.E.B. Bonfoh, Bassirou Kazwala, R. Ouma, C. Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_full | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_fullStr | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_full_unstemmed | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_short | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_sort | serological and molecular evidence of brucella species in the rapidly growing pig sector in kenya |
| topic | brucella swine serology |
| url | https://hdl.handle.net/10568/108242 |
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