CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding
Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/107838 |
| _version_ | 1855543745304330240 |
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| author | Tripathi, J.N. Ntui, V.O. Ron, M. Muiruri, S.K. Britt, A. Tripathi, L. |
| author_browse | Britt, A. Muiruri, S.K. Ntui, V.O. Ron, M. Tripathi, J.N. Tripathi, L. |
| author_facet | Tripathi, J.N. Ntui, V.O. Ron, M. Muiruri, S.K. Britt, A. Tripathi, L. |
| author_sort | Tripathi, J.N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one B genome, cannot be used as parents for crop improvement. Here, we report a strategy to inactivate the eBSV by editing the virus sequences. The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. Seventy-five percent of the edited events remained asymptomatic in comparison to the non-edited control plants under water stress conditions, confirming inactivation of eBSV into infectious viral particles. This study paves the way for the improvement of B genome germplasm and its use in breeding programs to produce hybrids that can be globally disseminated. |
| format | Journal Article |
| id | CGSpace107838 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1078382025-11-11T11:08:57Z CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding Tripathi, J.N. Ntui, V.O. Ron, M. Muiruri, S.K. Britt, A. Tripathi, L. bananas musa dna breeding viruses plantains genomes Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one B genome, cannot be used as parents for crop improvement. Here, we report a strategy to inactivate the eBSV by editing the virus sequences. The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. Seventy-five percent of the edited events remained asymptomatic in comparison to the non-edited control plants under water stress conditions, confirming inactivation of eBSV into infectious viral particles. This study paves the way for the improvement of B genome germplasm and its use in breeding programs to produce hybrids that can be globally disseminated. 2019 2020-03-24T10:18:23Z 2020-03-24T10:18:23Z Journal Article https://hdl.handle.net/10568/107838 en Open Access application/pdf Springer Tripathi, J.N., Ntui, V.O., Ron, M., Muiruri, S.K., Britt, A. & Tripathi, L. (2019). CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding. Communications Biology, 2(1), 1-11. |
| spellingShingle | bananas musa dna breeding viruses plantains genomes Tripathi, J.N. Ntui, V.O. Ron, M. Muiruri, S.K. Britt, A. Tripathi, L. CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding |
| title | CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding |
| title_full | CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding |
| title_fullStr | CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding |
| title_full_unstemmed | CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding |
| title_short | CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding |
| title_sort | crispr cas9 editing of endogenous banana streak virus in the b genome of musa spp overcomes a major challenge in banana breeding |
| topic | bananas musa dna breeding viruses plantains genomes |
| url | https://hdl.handle.net/10568/107838 |
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