Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield
Cassava is an important staple crop in sub‐Saharan Africa, due to its high productivity even on nutrient poor soils. The metabolic characteristics underlying this high productivity are poorly understood including the mode of photosynthesis, reasons for the high rate of photosynthesis, the extent of...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/107786 |
| _version_ | 1855517753969999872 |
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| author | Obata, T. Klemens, P.A.W. Rosado Souza, L. Schlereth, A. Gisel, A. Stavolone, L. Zierer, W. Morales, N. Muller, L.A. Zeeman, S.C. Ludewig, F. Stitt, M. Sonnewald, U. Neuhaus, H.E. Fernie, A.R. |
| author_browse | Fernie, A.R. Gisel, A. Klemens, P.A.W. Ludewig, F. Morales, N. Muller, L.A. Neuhaus, H.E. Obata, T. Rosado Souza, L. Schlereth, A. Sonnewald, U. Stavolone, L. Stitt, M. Zeeman, S.C. Zierer, W. |
| author_facet | Obata, T. Klemens, P.A.W. Rosado Souza, L. Schlereth, A. Gisel, A. Stavolone, L. Zierer, W. Morales, N. Muller, L.A. Zeeman, S.C. Ludewig, F. Stitt, M. Sonnewald, U. Neuhaus, H.E. Fernie, A.R. |
| author_sort | Obata, T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava is an important staple crop in sub‐Saharan Africa, due to its high productivity even on nutrient poor soils. The metabolic characteristics underlying this high productivity are poorly understood including the mode of photosynthesis, reasons for the high rate of photosynthesis, the extent of source/sink limitation, the impact of environment, and the extent of variation between cultivars. Six commercial African cassava cultivars were grown in a greenhouse in Erlangen, Germany, and in the field in Ibadan, Nigeria. Source leaves, sink leaves, stems and storage roots were harvested during storage root bulking and analyzed for sugars, organic acids, amino acids, phosphorylated intermediates, minerals, starch, protein, activities of enzymes in central metabolism and yield traits. High ratios of RuBisCO:phosphoenolpyruvate carboxylase activity support a C3 mode of photosynthesis. The high rate of photosynthesis is likely to be attributed to high activities of enzymes in the Calvin–Benson cycle and pathways for sucrose and starch synthesis. Nevertheless, source limitation is indicated because root yield traits correlated with metabolic traits in leaves rather than in the stem or storage roots. This situation was especially so in greenhouse‐grown plants, where irradiance will have been low. In the field, plants produced more storage roots. This was associated with higher AGPase activity and lower sucrose in the roots, indicating that feedforward loops enhanced sink capacity in the high light and low nitrogen environment in the field. Overall, these results indicated that carbon assimilation rate, the K battery, root starch synthesis, trehalose, and chlorogenic acid accumulation are potential target traits for genetic improvement. |
| format | Journal Article |
| id | CGSpace107786 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1077862025-11-11T10:06:45Z Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield Obata, T. Klemens, P.A.W. Rosado Souza, L. Schlereth, A. Gisel, A. Stavolone, L. Zierer, W. Morales, N. Muller, L.A. Zeeman, S.C. Ludewig, F. Stitt, M. Sonnewald, U. Neuhaus, H.E. Fernie, A.R. cassava yields photosynthesis carbon enzyme activity nitrogen metabolism starch cell biology genetics Cassava is an important staple crop in sub‐Saharan Africa, due to its high productivity even on nutrient poor soils. The metabolic characteristics underlying this high productivity are poorly understood including the mode of photosynthesis, reasons for the high rate of photosynthesis, the extent of source/sink limitation, the impact of environment, and the extent of variation between cultivars. Six commercial African cassava cultivars were grown in a greenhouse in Erlangen, Germany, and in the field in Ibadan, Nigeria. Source leaves, sink leaves, stems and storage roots were harvested during storage root bulking and analyzed for sugars, organic acids, amino acids, phosphorylated intermediates, minerals, starch, protein, activities of enzymes in central metabolism and yield traits. High ratios of RuBisCO:phosphoenolpyruvate carboxylase activity support a C3 mode of photosynthesis. The high rate of photosynthesis is likely to be attributed to high activities of enzymes in the Calvin–Benson cycle and pathways for sucrose and starch synthesis. Nevertheless, source limitation is indicated because root yield traits correlated with metabolic traits in leaves rather than in the stem or storage roots. This situation was especially so in greenhouse‐grown plants, where irradiance will have been low. In the field, plants produced more storage roots. This was associated with higher AGPase activity and lower sucrose in the roots, indicating that feedforward loops enhanced sink capacity in the high light and low nitrogen environment in the field. Overall, these results indicated that carbon assimilation rate, the K battery, root starch synthesis, trehalose, and chlorogenic acid accumulation are potential target traits for genetic improvement. 2020-06 2020-03-17T15:29:58Z 2020-03-17T15:29:58Z Journal Article https://hdl.handle.net/10568/107786 en Open Access application/pdf Wiley Obata, T., Klemens, P.A., Rosado‐Souza, L., Schlereth, A., Gisel, A., Stavolone, L., ... & Fernie, A.R. (2020). Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield. The Plant Journal, 1-40. |
| spellingShingle | cassava yields photosynthesis carbon enzyme activity nitrogen metabolism starch cell biology genetics Obata, T. Klemens, P.A.W. Rosado Souza, L. Schlereth, A. Gisel, A. Stavolone, L. Zierer, W. Morales, N. Muller, L.A. Zeeman, S.C. Ludewig, F. Stitt, M. Sonnewald, U. Neuhaus, H.E. Fernie, A.R. Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield |
| title | Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield |
| title_full | Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield |
| title_fullStr | Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield |
| title_full_unstemmed | Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield |
| title_short | Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield |
| title_sort | metabolic profiles of six african cultivars of cassava manihot esculenta crantz highlight bottlenecks of root yield |
| topic | cassava yields photosynthesis carbon enzyme activity nitrogen metabolism starch cell biology genetics |
| url | https://hdl.handle.net/10568/107786 |
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