Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture
Banana Bunchy Top Disease (BBTD) caused by the Banana Bunchy Top Virus (BBTV) is one of the most important banana diseases in the Democratic Republic of Congo. This study focused on the production of BBTV-free plantain seedlings from infected banana plants. A total of 10 suckers from the French plan...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Academic Journals
2020
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| Acceso en línea: | https://hdl.handle.net/10568/107726 |
| _version_ | 1855524998653935616 |
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| author | Tchatchambe, N.B.J. Ibanda, N. Adheka, G. Onautshu, O. Swennen, Rony L. Dhed’a, D. |
| author_browse | Adheka, G. Dhed’a, D. Ibanda, N. Onautshu, O. Swennen, Rony L. Tchatchambe, N.B.J. |
| author_facet | Tchatchambe, N.B.J. Ibanda, N. Adheka, G. Onautshu, O. Swennen, Rony L. Dhed’a, D. |
| author_sort | Tchatchambe, N.B.J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Banana Bunchy Top Disease (BBTD) caused by the Banana Bunchy Top Virus (BBTV) is one of the most important banana diseases in the Democratic Republic of Congo. This study focused on the production of BBTV-free plantain seedlings from infected banana plants. A total of 10 suckers from the French plantain Litete (Musa AAB) and the False Horn plantain Libanga Likale (Musa AAB) with advanced BBTD symptoms were collected. Meristematic apices excised from those suckers were cultured in vitro and subcultured five times. The presence of BBTV was evaluated by the Triple-Antibody Sandwich Enzyme-linked Immunosorbent Assay (TAS-ELISA). The BBTV was confirmed in all suckers prior to in vitro culture but 73.3% of Litete plantlets and 66.6% of Libanga Likale plantlets regenerated from meristematic tissues were virus-free. This indicates that in vitro culture is a simple tool to generate BBTV-free plantains. |
| format | Journal Article |
| id | CGSpace107726 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Academic Journals |
| publisherStr | Academic Journals |
| record_format | dspace |
| spelling | CGSpace1077262025-11-12T05:41:59Z Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture Tchatchambe, N.B.J. Ibanda, N. Adheka, G. Onautshu, O. Swennen, Rony L. Dhed’a, D. banana bunchy top virus in vitro culture tissue culture plantains Banana Bunchy Top Disease (BBTD) caused by the Banana Bunchy Top Virus (BBTV) is one of the most important banana diseases in the Democratic Republic of Congo. This study focused on the production of BBTV-free plantain seedlings from infected banana plants. A total of 10 suckers from the French plantain Litete (Musa AAB) and the False Horn plantain Libanga Likale (Musa AAB) with advanced BBTD symptoms were collected. Meristematic apices excised from those suckers were cultured in vitro and subcultured five times. The presence of BBTV was evaluated by the Triple-Antibody Sandwich Enzyme-linked Immunosorbent Assay (TAS-ELISA). The BBTV was confirmed in all suckers prior to in vitro culture but 73.3% of Litete plantlets and 66.6% of Libanga Likale plantlets regenerated from meristematic tissues were virus-free. This indicates that in vitro culture is a simple tool to generate BBTV-free plantains. 2020-03-31 2020-03-12T13:53:59Z 2020-03-12T13:53:59Z Journal Article https://hdl.handle.net/10568/107726 en Open Access application/pdf Academic Journals Tchatchambe, N.B.J., Ibanda, N., Adheka, J.G., Onautshu, O.D., Swennen, R. & Dhed'a, D.B. (2020). Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture. African Journal of Agricultural Research, 15(3), 361-366. |
| spellingShingle | banana bunchy top virus in vitro culture tissue culture plantains Tchatchambe, N.B.J. Ibanda, N. Adheka, G. Onautshu, O. Swennen, Rony L. Dhed’a, D. Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture |
| title | Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture |
| title_full | Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture |
| title_fullStr | Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture |
| title_full_unstemmed | Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture |
| title_short | Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture |
| title_sort | production of banana bunchy top virus bbtv free plantain plants by in vitro culture |
| topic | banana bunchy top virus in vitro culture tissue culture plantains |
| url | https://hdl.handle.net/10568/107726 |
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