Genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize-growing regions of Kenya
Highly toxigenic strains of Aspergillus flavus have been reported to frequently contaminate maize, causing fatal aflatoxin poisoning in Kenya. To gain insights into the environmental and genetic factors that influence toxigenicity, fungi (n = 218) that were culturally identified as A. flavus were is...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/107459 |
| _version_ | 1855524723360792576 |
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| author | Oloo, Richard Dooso Okoth, S. Wachira, P. Mutiga, Samuel K. Ochieng, Phillis Kago, Leah Ng'ang'a, Fredrick M. Domelevo Entfellner, Jean-Baka Ghimire, Sita R. |
| author_browse | Domelevo Entfellner, Jean-Baka Ghimire, Sita R. Kago, Leah Mutiga, Samuel K. Ng'ang'a, Fredrick M. Ochieng, Phillis Okoth, S. Oloo, Richard Dooso Wachira, P. |
| author_facet | Oloo, Richard Dooso Okoth, S. Wachira, P. Mutiga, Samuel K. Ochieng, Phillis Kago, Leah Ng'ang'a, Fredrick M. Domelevo Entfellner, Jean-Baka Ghimire, Sita R. |
| author_sort | Oloo, Richard Dooso |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Highly toxigenic strains of Aspergillus flavus have been reported to frequently contaminate maize, causing fatal aflatoxin poisoning in Kenya. To gain insights into the environmental and genetic factors that influence toxigenicity, fungi (n = 218) that were culturally identified as A. flavus were isolated from maize grains samples (n = 120) from three regions of Kenya. The fungi were further characterized to confirm their identities using a PCR-sequence analysis of the internal transcribed spacer (ITS) region of rDNA which also revealed all of them to be A. flavus. A subset of 72 isolates representing ITS sequence-based phylogeny cluster and the agroecological origin of maize samples was constituted for subsequent analysis. The analysis of partial calmodulin gene sequences showed that the subset consisted of A. flavus (87%) and Aspergillus minisclerotigenes (13%). No obvious association was detected between the presence of seven aflatoxin biosynthesis genes and fungal species or region. However, the presence of the aflD and aflS genes showed some association with aflatoxin production. The assessment of toxigenicity showed higher aflatoxin production potential in A. minisclerotigenes isolates. Given that A. minisclerotigenes were mainly observed in maize samples from Eastern Kenya, a known aflatoxin hotspot, we speculate that production of copious aflatoxin is an adaptative trait of this recently discovered species in the region. |
| format | Journal Article |
| id | CGSpace107459 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1074592024-05-01T08:17:16Z Genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize-growing regions of Kenya Oloo, Richard Dooso Okoth, S. Wachira, P. Mutiga, Samuel K. Ochieng, Phillis Kago, Leah Ng'ang'a, Fredrick M. Domelevo Entfellner, Jean-Baka Ghimire, Sita R. aspergillus flavus maize research Highly toxigenic strains of Aspergillus flavus have been reported to frequently contaminate maize, causing fatal aflatoxin poisoning in Kenya. To gain insights into the environmental and genetic factors that influence toxigenicity, fungi (n = 218) that were culturally identified as A. flavus were isolated from maize grains samples (n = 120) from three regions of Kenya. The fungi were further characterized to confirm their identities using a PCR-sequence analysis of the internal transcribed spacer (ITS) region of rDNA which also revealed all of them to be A. flavus. A subset of 72 isolates representing ITS sequence-based phylogeny cluster and the agroecological origin of maize samples was constituted for subsequent analysis. The analysis of partial calmodulin gene sequences showed that the subset consisted of A. flavus (87%) and Aspergillus minisclerotigenes (13%). No obvious association was detected between the presence of seven aflatoxin biosynthesis genes and fungal species or region. However, the presence of the aflD and aflS genes showed some association with aflatoxin production. The assessment of toxigenicity showed higher aflatoxin production potential in A. minisclerotigenes isolates. Given that A. minisclerotigenes were mainly observed in maize samples from Eastern Kenya, a known aflatoxin hotspot, we speculate that production of copious aflatoxin is an adaptative trait of this recently discovered species in the region. 2019-08-09 2020-03-11T07:13:06Z 2020-03-11T07:13:06Z Journal Article https://hdl.handle.net/10568/107459 en Open Access MDPI Oloo, R.D., Okoth, S., Wachira, P., Mutiga, S., Ochieng, P., Kago, L., Nganga, F., Domelevo Entfellner, J.-B. and Ghimire, S. 2019. Genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize-growing regions of Kenya. Toxins 11:467 |
| spellingShingle | aspergillus flavus maize research Oloo, Richard Dooso Okoth, S. Wachira, P. Mutiga, Samuel K. Ochieng, Phillis Kago, Leah Ng'ang'a, Fredrick M. Domelevo Entfellner, Jean-Baka Ghimire, Sita R. Genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize-growing regions of Kenya |
| title | Genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize-growing regions of Kenya |
| title_full | Genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize-growing regions of Kenya |
| title_fullStr | Genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize-growing regions of Kenya |
| title_full_unstemmed | Genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize-growing regions of Kenya |
| title_short | Genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize-growing regions of Kenya |
| title_sort | genetic profiling of aspergillus isolates with varying aflatoxin production potential from different maize growing regions of kenya |
| topic | aspergillus flavus maize research |
| url | https://hdl.handle.net/10568/107459 |
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