The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses
Fourth-generation cephalosporins can select for extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in horses, but it is unknown to what extent this occurs compared to penicillin/gentamicin combination treatment. The objective was to evaluate the effect of different antimicrobial treat...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/107420 |
| _version_ | 1855521941926969344 |
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| author | Schoster, A. Spijk, J.N. van Damborg, P. Moodley, Arshnee Kirchgaessner, C. Hartnack, S. Schmitt, S. |
| author_browse | Damborg, P. Hartnack, S. Kirchgaessner, C. Moodley, Arshnee Schmitt, S. Schoster, A. Spijk, J.N. van |
| author_facet | Schoster, A. Spijk, J.N. van Damborg, P. Moodley, Arshnee Kirchgaessner, C. Hartnack, S. Schmitt, S. |
| author_sort | Schoster, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Fourth-generation cephalosporins can select for extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in horses, but it is unknown to what extent this occurs compared to penicillin/gentamicin combination treatment. The objective was to evaluate the effect of different antimicrobial treatments on faecal shedding and diversity of ESBL-producing Escherichia coli (ESBL-EC) in horses. Upon hospital admission, 86 horses in need of antimicrobial treatment or prophylaxis were randomly allocated to receive penicillin and gentamicin (PG) or cefquinome (CEF). Untreated horses were included as controls (NOAMD, n = 33). Faecal samples from admission (T1), 3 days after admission (T2), and faecal swabs 28 days after discharge (T3) were cultured selectively. Differences in prevalence (T1, T2, T3) and counts (T1, T2) of ESBL-EC between groups and over time were analysed. On a subset of ESBL-EC isolates, antimicrobial susceptibility testing (n = 45) and whole-genome sequencing followed by SNP-analysis (n = 46) were performed. The prevalence of ESBL-EC at T1 was 12 % with no significant difference between groups. In all groups, significantly higher carriage rates were observed at T2 and T3 compared to T1. Carriage and counts of ESBL-EC at T2 were significantly higher in treated compared to untreated horses. There was no significant difference between PG and CEF at any time points. Despite a large genetic diversity, indistinguishable ESBL clones were observed in different horses over time. In conclusion, antimicrobial treatment and hospitalization increased prevalence and counts of ESBL-EC, and transmission of ESBL-EC in the hospital was suspected. These findings highlight the importance of antimicrobial stewardship and infection control practices in equine medicine. |
| format | Journal Article |
| id | CGSpace107420 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1074202024-05-01T08:16:37Z The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses Schoster, A. Spijk, J.N. van Damborg, P. Moodley, Arshnee Kirchgaessner, C. Hartnack, S. Schmitt, S. antimicrobials horses Fourth-generation cephalosporins can select for extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in horses, but it is unknown to what extent this occurs compared to penicillin/gentamicin combination treatment. The objective was to evaluate the effect of different antimicrobial treatments on faecal shedding and diversity of ESBL-producing Escherichia coli (ESBL-EC) in horses. Upon hospital admission, 86 horses in need of antimicrobial treatment or prophylaxis were randomly allocated to receive penicillin and gentamicin (PG) or cefquinome (CEF). Untreated horses were included as controls (NOAMD, n = 33). Faecal samples from admission (T1), 3 days after admission (T2), and faecal swabs 28 days after discharge (T3) were cultured selectively. Differences in prevalence (T1, T2, T3) and counts (T1, T2) of ESBL-EC between groups and over time were analysed. On a subset of ESBL-EC isolates, antimicrobial susceptibility testing (n = 45) and whole-genome sequencing followed by SNP-analysis (n = 46) were performed. The prevalence of ESBL-EC at T1 was 12 % with no significant difference between groups. In all groups, significantly higher carriage rates were observed at T2 and T3 compared to T1. Carriage and counts of ESBL-EC at T2 were significantly higher in treated compared to untreated horses. There was no significant difference between PG and CEF at any time points. Despite a large genetic diversity, indistinguishable ESBL clones were observed in different horses over time. In conclusion, antimicrobial treatment and hospitalization increased prevalence and counts of ESBL-EC, and transmission of ESBL-EC in the hospital was suspected. These findings highlight the importance of antimicrobial stewardship and infection control practices in equine medicine. 2020-04 2020-03-09T13:09:51Z 2020-03-09T13:09:51Z Journal Article https://hdl.handle.net/10568/107420 en Limited Access Elsevier Schoster, A., Spijk, J.N. van, Damborg, P., Moodley, A., Kirchgaessner, C., Hartnack, S. and Schmitt, S. 2020. The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses. Veterinary Microbiology 243: 108617. |
| spellingShingle | antimicrobials horses Schoster, A. Spijk, J.N. van Damborg, P. Moodley, Arshnee Kirchgaessner, C. Hartnack, S. Schmitt, S. The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses |
| title | The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses |
| title_full | The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses |
| title_fullStr | The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses |
| title_full_unstemmed | The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses |
| title_short | The effect of different antimicrobial treatment regimens on the faecal shedding of ESBL-producing Escherichia coli in horses |
| title_sort | effect of different antimicrobial treatment regimens on the faecal shedding of esbl producing escherichia coli in horses |
| topic | antimicrobials horses |
| url | https://hdl.handle.net/10568/107420 |
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