Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties.
Three late blight resistance (R) genes from wild potato species were transferred as a stack into the farmer-preferred varieties ‘Tigoni’ and ‘Shangi’. Transgenic events were tested by detached-leaf assays (DLA) and whole-plant assays (WPA) with isolates of Phytophthora infestans using 20,000 sporang...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Academic Journals
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/107143 |
| _version_ | 1855514435630661632 |
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| author | Webi, E.N. Kariuki, D. Kinyua, J. Njoroge, A.W. Ghislain, M. Magembe, E. |
| author_browse | Ghislain, M. Kariuki, D. Kinyua, J. Magembe, E. Njoroge, A.W. Webi, E.N. |
| author_facet | Webi, E.N. Kariuki, D. Kinyua, J. Njoroge, A.W. Ghislain, M. Magembe, E. |
| author_sort | Webi, E.N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Three late blight resistance (R) genes from wild potato species were transferred as a stack into the farmer-preferred varieties ‘Tigoni’ and ‘Shangi’. Transgenic events were tested by detached-leaf assays (DLA) and whole-plant assays (WPA) with isolates of Phytophthora infestans using 20,000 sporangia / mL inoculum. For the first DLA, 9 out of 13 ‘Tigoni’ and 10 out of 12 ‘Shangi’ transgenic events had scores below 3% of leaf affected area. 17 of the 19 transgenic events with low scores were subjected to a second DLA using two different P. infestans isolates. 8 of them exhibited extreme LB resistance while the remaining 9 transgenic events showed hypersensitive response (HR). 6 transgenic events (2 ‘Tigoni’ and 4 ‘Shangi’) with extreme resistance by DLA were subjected to WPA and they all exhibited extreme resistance to LB. Hence, this study demonstrates that a simple DLA predicts high level of resistance to late blight. R gene expression analysis in 18 transgenic events showed different transgenic events exhibiting different levels of expression in the three genes. However, that pathogen induction and / or high R gene expression are necessary for extreme resistance when transgenic events bear a stack of three R genes was not observed. |
| format | Journal Article |
| id | CGSpace107143 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Academic Journals |
| publisherStr | Academic Journals |
| record_format | dspace |
| spelling | CGSpace1071432025-03-13T09:46:13Z Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties. Webi, E.N. Kariuki, D. Kinyua, J. Njoroge, A.W. Ghislain, M. Magembe, E. phytophthora infestans genes potatoes disease resistance Three late blight resistance (R) genes from wild potato species were transferred as a stack into the farmer-preferred varieties ‘Tigoni’ and ‘Shangi’. Transgenic events were tested by detached-leaf assays (DLA) and whole-plant assays (WPA) with isolates of Phytophthora infestans using 20,000 sporangia / mL inoculum. For the first DLA, 9 out of 13 ‘Tigoni’ and 10 out of 12 ‘Shangi’ transgenic events had scores below 3% of leaf affected area. 17 of the 19 transgenic events with low scores were subjected to a second DLA using two different P. infestans isolates. 8 of them exhibited extreme LB resistance while the remaining 9 transgenic events showed hypersensitive response (HR). 6 transgenic events (2 ‘Tigoni’ and 4 ‘Shangi’) with extreme resistance by DLA were subjected to WPA and they all exhibited extreme resistance to LB. Hence, this study demonstrates that a simple DLA predicts high level of resistance to late blight. R gene expression analysis in 18 transgenic events showed different transgenic events exhibiting different levels of expression in the three genes. However, that pathogen induction and / or high R gene expression are necessary for extreme resistance when transgenic events bear a stack of three R genes was not observed. 2019-09 2020-02-19T01:05:14Z 2020-02-19T01:05:14Z Journal Article https://hdl.handle.net/10568/107143 en Open Access Academic Journals Webi, E. N.; Kariuki, D.; Kinyua, J.; Njoroge, A.; Ghislain, M.; Magembe, E. 2019. Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties. African Journal of Biotechnology. ISSN: 1684-5315. 18(29). pp. 845-856. |
| spellingShingle | phytophthora infestans genes potatoes disease resistance Webi, E.N. Kariuki, D. Kinyua, J. Njoroge, A.W. Ghislain, M. Magembe, E. Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties. |
| title | Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties. |
| title_full | Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties. |
| title_fullStr | Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties. |
| title_full_unstemmed | Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties. |
| title_short | Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties. |
| title_sort | extreme resistance to late blight disease by transferring 3 r genes from wild relatives into african farmer preferred potato varieties |
| topic | phytophthora infestans genes potatoes disease resistance |
| url | https://hdl.handle.net/10568/107143 |
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