Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid
Background: Whiteflies are a threat to cassava (Manihot esculenta), an important staple food in many tropical/ subtropical regions. Understanding the molecular mechanisms regulating cassava’s responses against this pest is crucial for developing control strategies. Pathogenesis-related (PR) protein...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Springer
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/107040 |
| _version_ | 1855539310833434624 |
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| author | Irigoyen, Maria L. Garceau, Danielle C Bohórquez Cháux, Adriana Becerra López Lavelle, Luis Augusto Pérez Fons, Laura Fraser, Paul D. Walling, Linda L. |
| author_browse | Becerra López Lavelle, Luis Augusto Bohórquez Cháux, Adriana Fraser, Paul D. Garceau, Danielle C Irigoyen, Maria L. Pérez Fons, Laura Walling, Linda L. |
| author_facet | Irigoyen, Maria L. Garceau, Danielle C Bohórquez Cháux, Adriana Becerra López Lavelle, Luis Augusto Pérez Fons, Laura Fraser, Paul D. Walling, Linda L. |
| author_sort | Irigoyen, Maria L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: Whiteflies are a threat to cassava (Manihot esculenta), an important staple food in many tropical/ subtropical regions. Understanding the molecular mechanisms regulating cassava’s responses against this pest is crucial for developing control strategies. Pathogenesis-related (PR) protein families are an integral part of plant immunity. With the availability of whole genome sequences, the annotation and expression programs of the full complement of PR genes in an organism can now be achieved. An understanding of the responses of the entire complement of PR genes during biotic stress and to the defense hormones, salicylic acid (SA) and jasmonic acid (JA), is lacking. Here, we analyze the responses of cassava PR genes to whiteflies, SA, JA, and other biotic aggressors.
Results: The cassava genome possesses 14 of the 17 plant PR families, with a total of 447 PR genes. A cassava PR gene nomenclature is proposed. Phylogenetic relatedness of cassava PR proteins to each other and to homologs in poplar, rice and Arabidopsis identified cassava-specific PR gene family expansions. The temporal programs of PR gene expression in response to the whitefly (Aleurotrachelus socialis) in four whitefly-susceptible cassava genotypes showed that 167 of the 447 PR genes were regulated after whitefly infestation. While the timing of PR gene expression varied, over 37% of whitefly-regulated PR genes were downregulated in all four genotypes. Notably, whitefly-responsive PR genes were largely coordinately regulated by SA and JA. The analysis of cassava PR gene expression in response to five other biotic stresses revealed a strong positive correlation between whitefly and Xanthomonas axonopodis and Cassava Brown Streak Virus responses and negative correlations between whitefly and Cassava Mosaic Virus responses. Finally, certain associations between PR genes in cassava expansions and response to biotic stresses were observed among PR families.
Conclusions: This study represents the first genome-wide characterization of PR genes in cassava. PR gene responses to six biotic stresses and to SA and JA are demonstrably different to other angiosperms. We propose that our approach could be applied in other species to fully understand PR gene regulation by pathogens, pests and the canonical defense hormones SA and JA. |
| format | Journal Article |
| id | CGSpace107040 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1070402025-11-12T05:59:25Z Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid Irigoyen, Maria L. Garceau, Danielle C Bohórquez Cháux, Adriana Becerra López Lavelle, Luis Augusto Pérez Fons, Laura Fraser, Paul D. Walling, Linda L. cassava jasmonic acid pathogenesis analysis manihot esculenta mandioca acido jasmonico patogenesis Background: Whiteflies are a threat to cassava (Manihot esculenta), an important staple food in many tropical/ subtropical regions. Understanding the molecular mechanisms regulating cassava’s responses against this pest is crucial for developing control strategies. Pathogenesis-related (PR) protein families are an integral part of plant immunity. With the availability of whole genome sequences, the annotation and expression programs of the full complement of PR genes in an organism can now be achieved. An understanding of the responses of the entire complement of PR genes during biotic stress and to the defense hormones, salicylic acid (SA) and jasmonic acid (JA), is lacking. Here, we analyze the responses of cassava PR genes to whiteflies, SA, JA, and other biotic aggressors. Results: The cassava genome possesses 14 of the 17 plant PR families, with a total of 447 PR genes. A cassava PR gene nomenclature is proposed. Phylogenetic relatedness of cassava PR proteins to each other and to homologs in poplar, rice and Arabidopsis identified cassava-specific PR gene family expansions. The temporal programs of PR gene expression in response to the whitefly (Aleurotrachelus socialis) in four whitefly-susceptible cassava genotypes showed that 167 of the 447 PR genes were regulated after whitefly infestation. While the timing of PR gene expression varied, over 37% of whitefly-regulated PR genes were downregulated in all four genotypes. Notably, whitefly-responsive PR genes were largely coordinately regulated by SA and JA. The analysis of cassava PR gene expression in response to five other biotic stresses revealed a strong positive correlation between whitefly and Xanthomonas axonopodis and Cassava Brown Streak Virus responses and negative correlations between whitefly and Cassava Mosaic Virus responses. Finally, certain associations between PR genes in cassava expansions and response to biotic stresses were observed among PR families. Conclusions: This study represents the first genome-wide characterization of PR genes in cassava. PR gene responses to six biotic stresses and to SA and JA are demonstrably different to other angiosperms. We propose that our approach could be applied in other species to fully understand PR gene regulation by pathogens, pests and the canonical defense hormones SA and JA. 2020-12 2020-02-11T15:22:04Z 2020-02-11T15:22:04Z Journal Article https://hdl.handle.net/10568/107040 en Open Access application/pdf Springer Irigoyen, Maria L.; Garceau, Danielle C.; Bohorquez-Chaux, Adriana; Becerra Lopez-Lavalle, Luis Augusto; Perez-Fons, Laura; Fraser, Paul D.; Walling, Linda L. (2020) Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid. BMC Genomics 21:93 18 p. ISSN: 1471-2164 |
| spellingShingle | cassava jasmonic acid pathogenesis analysis manihot esculenta mandioca acido jasmonico patogenesis Irigoyen, Maria L. Garceau, Danielle C Bohórquez Cháux, Adriana Becerra López Lavelle, Luis Augusto Pérez Fons, Laura Fraser, Paul D. Walling, Linda L. Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid |
| title | Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid |
| title_full | Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid |
| title_fullStr | Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid |
| title_full_unstemmed | Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid |
| title_short | Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid |
| title_sort | genome wide analyses of cassava pathogenesis related pr gene families reveal core transcriptome responses to whitefly infestation salicylic acid and jasmonic acid |
| topic | cassava jasmonic acid pathogenesis analysis manihot esculenta mandioca acido jasmonico patogenesis |
| url | https://hdl.handle.net/10568/107040 |
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