Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions
Increasing drought resistance without sacrificing grain yield remains an ongoing challenge in crop improvement. In this study, we report that Oryza sativa CCCH‐tandem zinc finger protein 5 (OsTZF5) can confer drought resistance and increase grain yield in transgenic rice plants. Expression of OsTZF5...
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2020
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/107023 |
| _version_ | 1855526812418834432 |
|---|---|
| author | Selvaraj, Michael Gomez Jan, Asad Ishizaki, Takuma Valencia Ortiz, Milton Orlando Dedicova, Beata Maruyama, Kyonoshin Ogata, Takuya Todaka, Daisuke Yamaguchi-Shinozaki, Kazuko Nakashima, Kazuo Ishitani, Manabu |
| author_browse | Dedicova, Beata Ishitani, Manabu Ishizaki, Takuma Jan, Asad Maruyama, Kyonoshin Nakashima, Kazuo Ogata, Takuya Selvaraj, Michael Gomez Todaka, Daisuke Valencia Ortiz, Milton Orlando Yamaguchi-Shinozaki, Kazuko |
| author_facet | Selvaraj, Michael Gomez Jan, Asad Ishizaki, Takuma Valencia Ortiz, Milton Orlando Dedicova, Beata Maruyama, Kyonoshin Ogata, Takuya Todaka, Daisuke Yamaguchi-Shinozaki, Kazuko Nakashima, Kazuo Ishitani, Manabu |
| author_sort | Selvaraj, Michael Gomez |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Increasing drought resistance without sacrificing grain yield remains an ongoing challenge in crop improvement. In this study, we report that Oryza sativa CCCH‐tandem zinc finger protein 5 (OsTZF5) can confer drought resistance and increase grain yield in transgenic rice plants. Expression of OsTZF5 was induced by abscisic acid, dehydration and cold stress. Upon stress, OsTZF5‐GFP localized to the cytoplasm and cytoplasmic foci. Transgenic rice plants overexpressing OsTZF5 under the constitutive maize ubiquitin promoter exhibited improved survival under drought but also growth retardation. By introducing OsTZF5 behind the stress‐responsive OsNAC6 promoter in two commercial upland cultivars, Curinga and NERICA4, we obtained transgenic plants that showed no growth retardation. Moreover, these plants exhibited significantly increased grain yield compared to non‐transgenic cultivars in different confined field drought environments. Physiological analysis indicated that OsTZF5 promoted both drought tolerance and drought avoidance. Collectively, our results provide strong evidence that OsTZF5 is a useful biotechnological tool to minimize yield losses in rice grown under drought conditions. |
| format | Journal Article |
| id | CGSpace107023 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1070232025-11-12T05:55:43Z Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions Selvaraj, Michael Gomez Jan, Asad Ishizaki, Takuma Valencia Ortiz, Milton Orlando Dedicova, Beata Maruyama, Kyonoshin Ogata, Takuya Todaka, Daisuke Yamaguchi-Shinozaki, Kazuko Nakashima, Kazuo Ishitani, Manabu drought rice transgenics transgénicos sequia arroz Increasing drought resistance without sacrificing grain yield remains an ongoing challenge in crop improvement. In this study, we report that Oryza sativa CCCH‐tandem zinc finger protein 5 (OsTZF5) can confer drought resistance and increase grain yield in transgenic rice plants. Expression of OsTZF5 was induced by abscisic acid, dehydration and cold stress. Upon stress, OsTZF5‐GFP localized to the cytoplasm and cytoplasmic foci. Transgenic rice plants overexpressing OsTZF5 under the constitutive maize ubiquitin promoter exhibited improved survival under drought but also growth retardation. By introducing OsTZF5 behind the stress‐responsive OsNAC6 promoter in two commercial upland cultivars, Curinga and NERICA4, we obtained transgenic plants that showed no growth retardation. Moreover, these plants exhibited significantly increased grain yield compared to non‐transgenic cultivars in different confined field drought environments. Physiological analysis indicated that OsTZF5 promoted both drought tolerance and drought avoidance. Collectively, our results provide strong evidence that OsTZF5 is a useful biotechnological tool to minimize yield losses in rice grown under drought conditions. 2020-08 2020-02-10T21:05:39Z 2020-02-10T21:05:39Z Journal Article https://hdl.handle.net/10568/107023 en Open Access application/pdf Wiley Selvaraj, M.G.; Jan, A.; Ishizaki, T.; Valencia, M. ; Dedicova, B.; Maruyama, K.; Ogata, T.; Todaka, D.; Yamaguchi-Shinozaki, K.; Nakashima, K.; Ishitani, M. (2020) Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions. Plant Biotechnology Journal 18(8) p. 1711-1721. ISSN: 1467-7652 |
| spellingShingle | drought rice transgenics transgénicos sequia arroz Selvaraj, Michael Gomez Jan, Asad Ishizaki, Takuma Valencia Ortiz, Milton Orlando Dedicova, Beata Maruyama, Kyonoshin Ogata, Takuya Todaka, Daisuke Yamaguchi-Shinozaki, Kazuko Nakashima, Kazuo Ishitani, Manabu Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions |
| title | Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions |
| title_full | Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions |
| title_fullStr | Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions |
| title_full_unstemmed | Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions |
| title_short | Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions |
| title_sort | expression of the ccch tandem zinc finger protein gene ostzf5 under a stress inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions |
| topic | drought rice transgenics transgénicos sequia arroz |
| url | https://hdl.handle.net/10568/107023 |
| work_keys_str_mv | AT selvarajmichaelgomez expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT janasad expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT ishizakitakuma expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT valenciaortizmiltonorlando expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT dedicovabeata expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT maruyamakyonoshin expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT ogatatakuya expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT todakadaisuke expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT yamaguchishinozakikazuko expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT nakashimakazuo expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions AT ishitanimanabu expressionoftheccchtandemzincfingerproteingeneostzf5underastressinduciblepromotermitigatestheeffectofdroughtstressonricegrainyieldunderfieldconditions |