A mini-atlas of gene expression for the domestic goat (Capra hircus)

Goats (Capra hircus) are an economically important livestock species providing meat and milk across the globe. They are of particular importance in tropical agri-systems contributing to sustainable agriculture, alleviation of poverty, social cohesion and utilisation of marginal grazing. There are ex...

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Autores principales: Muriuki, C., Bush, S.J., Salavati, M., McCulloch, M.E.B., Lisowski, Z.M., Agaba, Morris, Djikeng, Appolinaire, Hume, D.A., Clark, E.L.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Frontiers Media 2019
Materias:
Acceso en línea:https://hdl.handle.net/10568/106334
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author Muriuki, C.
Bush, S.J.
Salavati, M.
McCulloch, M.E.B.
Lisowski, Z.M.
Agaba, Morris
Djikeng, Appolinaire
Hume, D.A.
Clark, E.L.
author_browse Agaba, Morris
Bush, S.J.
Clark, E.L.
Djikeng, Appolinaire
Hume, D.A.
Lisowski, Z.M.
McCulloch, M.E.B.
Muriuki, C.
Salavati, M.
author_facet Muriuki, C.
Bush, S.J.
Salavati, M.
McCulloch, M.E.B.
Lisowski, Z.M.
Agaba, Morris
Djikeng, Appolinaire
Hume, D.A.
Clark, E.L.
author_sort Muriuki, C.
collection Repository of Agricultural Research Outputs (CGSpace)
description Goats (Capra hircus) are an economically important livestock species providing meat and milk across the globe. They are of particular importance in tropical agri-systems contributing to sustainable agriculture, alleviation of poverty, social cohesion and utilisation of marginal grazing. There are excellent genetic and genomic resources available for goats, including a highly contiguous reference genome (ARS1). However, gene expression information is limited in comparison to other ruminants. To support functional annotation of the genome and comparative transcriptomics we created a mini-atlas of gene expression for the domestic goat. RNA-Seq analysis of 22 transcriptionally rich tissues and cell-types detected the majority (90%) of predicted protein-coding transcripts and assigned informative gene names to more than 1000 previously unannotated protein-coding genes in the current reference genome for goat (ARS1). Using network-based cluster analysis we grouped genes according to their expression patterns and assigned those groups of co-expressed genes to specific cell populations or pathways. We describe clusters of genes expressed in the gastro-intestinal tract and provide the expression profiles across tissues of a subset of genes associated with functional traits. Comparative analysis of the goat atlas with the larger sheep gene expression atlas dataset revealed transcriptional similarities between macrophage-associated signatures in the sheep and goats sampled in this study. The goat transcriptomic resource complements the large gene expression dataset we have generated for sheep and contributes to the available genomic resources for interpretation of the relationship between genotype and phenotype in small ruminants.
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spelling CGSpace1063342024-10-03T07:40:47Z A mini-atlas of gene expression for the domestic goat (Capra hircus) Muriuki, C. Bush, S.J. Salavati, M. McCulloch, M.E.B. Lisowski, Z.M. Agaba, Morris Djikeng, Appolinaire Hume, D.A. Clark, E.L. animal breeding genetics goats small ruminants Goats (Capra hircus) are an economically important livestock species providing meat and milk across the globe. They are of particular importance in tropical agri-systems contributing to sustainable agriculture, alleviation of poverty, social cohesion and utilisation of marginal grazing. There are excellent genetic and genomic resources available for goats, including a highly contiguous reference genome (ARS1). However, gene expression information is limited in comparison to other ruminants. To support functional annotation of the genome and comparative transcriptomics we created a mini-atlas of gene expression for the domestic goat. RNA-Seq analysis of 22 transcriptionally rich tissues and cell-types detected the majority (90%) of predicted protein-coding transcripts and assigned informative gene names to more than 1000 previously unannotated protein-coding genes in the current reference genome for goat (ARS1). Using network-based cluster analysis we grouped genes according to their expression patterns and assigned those groups of co-expressed genes to specific cell populations or pathways. We describe clusters of genes expressed in the gastro-intestinal tract and provide the expression profiles across tissues of a subset of genes associated with functional traits. Comparative analysis of the goat atlas with the larger sheep gene expression atlas dataset revealed transcriptional similarities between macrophage-associated signatures in the sheep and goats sampled in this study. The goat transcriptomic resource complements the large gene expression dataset we have generated for sheep and contributes to the available genomic resources for interpretation of the relationship between genotype and phenotype in small ruminants. 2019-11-04 2019-12-26T08:39:02Z 2019-12-26T08:39:02Z Journal Article https://hdl.handle.net/10568/106334 en Open Access Frontiers Media Muriuki, C., Bush, S.J., Salavati, M., McCulloch, M.E.B., Lisowski, Z.M., Agaba, M., Djikeng, A., Hume, D.A. and Clark, E.L. 2019. A mini-atlas of gene expression for the domestic goat (Capra hircus). Frontiers in Genetics 10:1080.
spellingShingle animal breeding
genetics
goats
small ruminants
Muriuki, C.
Bush, S.J.
Salavati, M.
McCulloch, M.E.B.
Lisowski, Z.M.
Agaba, Morris
Djikeng, Appolinaire
Hume, D.A.
Clark, E.L.
A mini-atlas of gene expression for the domestic goat (Capra hircus)
title A mini-atlas of gene expression for the domestic goat (Capra hircus)
title_full A mini-atlas of gene expression for the domestic goat (Capra hircus)
title_fullStr A mini-atlas of gene expression for the domestic goat (Capra hircus)
title_full_unstemmed A mini-atlas of gene expression for the domestic goat (Capra hircus)
title_short A mini-atlas of gene expression for the domestic goat (Capra hircus)
title_sort mini atlas of gene expression for the domestic goat capra hircus
topic animal breeding
genetics
goats
small ruminants
url https://hdl.handle.net/10568/106334
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