Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels
Shiga toxin-producing Escherichia coli (STEC) cause gastrointestinal illnesses including non-bloody or bloody diarrhoea, haemorrhagic colitis (HC), and the haemolytic uremic syndrome (HUS). To investigate the occurrence of STEC among grazing dromedaries from Kenya, E. coli isolated from fecal matter...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/106276 |
| _version_ | 1855535097488343040 |
|---|---|
| author | Baschera, M. Cernela, N. Stevens, M.J.A. Liljander, Anne M. Jores, Joerg Max Corman, V. Nüesch-Inderbinen, M. Stephan, R. |
| author_browse | Baschera, M. Cernela, N. Jores, Joerg Liljander, Anne M. Max Corman, V. Nüesch-Inderbinen, M. Stephan, R. Stevens, M.J.A. |
| author_facet | Baschera, M. Cernela, N. Stevens, M.J.A. Liljander, Anne M. Jores, Joerg Max Corman, V. Nüesch-Inderbinen, M. Stephan, R. |
| author_sort | Baschera, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Shiga toxin-producing Escherichia coli (STEC) cause gastrointestinal illnesses including non-bloody or bloody diarrhoea, haemorrhagic colitis (HC), and the haemolytic uremic syndrome (HUS). To investigate the occurrence of STEC among grazing dromedaries from Kenya, E. coli isolated from fecal matter collected from 163 dromedaries on a large ranch were screened for the presence of stx1 and stx2. STEC strains were isolated and serotyped. Isolates were subjected to PCR for the subtyping of stx genes and for the detection of eae and ehx. In addition, whole genome sequencing (WGS) was carried out to detect further virulence genes and to determine the multilocus sequence types (MLST). Antimicrobial resistance profiles were determined by disk diffusion.
STEC was isolated from 20 (12.3%) of the fecal samples. Thereof, nine (45%) isolates were STEC O156:H25, three (15%) isolates typed STEC O43:H2. The remaining isolates occurred as single serotypes or were O non-typeable. Eleven (55%) of the isolates harboured stx2a, nine (45%) eae, and 14 (70%) ehx, respectively. WGS revealed the presence of iss in 16 (80%), subAB in four (20%) and astA in two (10%) of the isolates, Furthermore, espA, tccP, nleA, nleB, tccP, and tir were found exclusively among STEC O156:H25.
Eleven different sequence types (ST) were detected. The most prominent was ST300/ST5343, which comprised STEC O156:H25. All STEC isolates were pan susceptible to a panel of 16 antimicrobial agents. Overall, the results indicate that dromedary camels in Kenya may be reservoirs of STEC, including serotypes possessing virulence markers associated to disease in humans, such as STEC O156:H25. STEC in camels may represent a health hazard for humans with close contact to camels or to consumers of camel derived foodstuffs, such as unpasteurised camel milk. |
| format | Journal Article |
| id | CGSpace106276 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1062762024-05-01T08:18:53Z Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels Baschera, M. Cernela, N. Stevens, M.J.A. Liljander, Anne M. Jores, Joerg Max Corman, V. Nüesch-Inderbinen, M. Stephan, R. camels genomes research antimicrobial resistance health food safety Shiga toxin-producing Escherichia coli (STEC) cause gastrointestinal illnesses including non-bloody or bloody diarrhoea, haemorrhagic colitis (HC), and the haemolytic uremic syndrome (HUS). To investigate the occurrence of STEC among grazing dromedaries from Kenya, E. coli isolated from fecal matter collected from 163 dromedaries on a large ranch were screened for the presence of stx1 and stx2. STEC strains were isolated and serotyped. Isolates were subjected to PCR for the subtyping of stx genes and for the detection of eae and ehx. In addition, whole genome sequencing (WGS) was carried out to detect further virulence genes and to determine the multilocus sequence types (MLST). Antimicrobial resistance profiles were determined by disk diffusion. STEC was isolated from 20 (12.3%) of the fecal samples. Thereof, nine (45%) isolates were STEC O156:H25, three (15%) isolates typed STEC O43:H2. The remaining isolates occurred as single serotypes or were O non-typeable. Eleven (55%) of the isolates harboured stx2a, nine (45%) eae, and 14 (70%) ehx, respectively. WGS revealed the presence of iss in 16 (80%), subAB in four (20%) and astA in two (10%) of the isolates, Furthermore, espA, tccP, nleA, nleB, tccP, and tir were found exclusively among STEC O156:H25. Eleven different sequence types (ST) were detected. The most prominent was ST300/ST5343, which comprised STEC O156:H25. All STEC isolates were pan susceptible to a panel of 16 antimicrobial agents. Overall, the results indicate that dromedary camels in Kenya may be reservoirs of STEC, including serotypes possessing virulence markers associated to disease in humans, such as STEC O156:H25. STEC in camels may represent a health hazard for humans with close contact to camels or to consumers of camel derived foodstuffs, such as unpasteurised camel milk. 2019-06 2019-12-20T08:00:39Z 2019-12-20T08:00:39Z Journal Article https://hdl.handle.net/10568/106276 en Open Access Elsevier Baschera, M., Cernela, N., Stevens, M.J.A., Liljander, A., Jores, J., Max Corman, V., Nüesch-Inderbinen, M. and Stephan, R. 2019. Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels. One Health 7: 100087. |
| spellingShingle | camels genomes research antimicrobial resistance health food safety Baschera, M. Cernela, N. Stevens, M.J.A. Liljander, Anne M. Jores, Joerg Max Corman, V. Nüesch-Inderbinen, M. Stephan, R. Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_full | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_fullStr | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_full_unstemmed | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_short | Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels |
| title_sort | shiga toxin producing escherichia coli stec isolated from fecal samples of african dromedary camels |
| topic | camels genomes research antimicrobial resistance health food safety |
| url | https://hdl.handle.net/10568/106276 |
| work_keys_str_mv | AT bascheram shigatoxinproducingescherichiacolistecisolatedfromfecalsamplesofafricandromedarycamels AT cernelan shigatoxinproducingescherichiacolistecisolatedfromfecalsamplesofafricandromedarycamels AT stevensmja shigatoxinproducingescherichiacolistecisolatedfromfecalsamplesofafricandromedarycamels AT liljanderannem shigatoxinproducingescherichiacolistecisolatedfromfecalsamplesofafricandromedarycamels AT joresjoerg shigatoxinproducingescherichiacolistecisolatedfromfecalsamplesofafricandromedarycamels AT maxcormanv shigatoxinproducingescherichiacolistecisolatedfromfecalsamplesofafricandromedarycamels AT nueschinderbinenm shigatoxinproducingescherichiacolistecisolatedfromfecalsamplesofafricandromedarycamels AT stephanr shigatoxinproducingescherichiacolistecisolatedfromfecalsamplesofafricandromedarycamels |