Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.)
Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis “DH Pahang” to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes we...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Frontiers Media
2019
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/106239 |
| _version_ | 1855543060816986112 |
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| author | Šimoníková, D. Němečková, A. Karafiátová, M. Uwimana, Brigitte Swennen, Rony L. Doležel, Jaroslav Hřibová, E. |
| author_browse | Doležel, Jaroslav Hřibová, E. Karafiátová, M. Němečková, A. Swennen, Rony L. Uwimana, Brigitte Šimoníková, D. |
| author_facet | Šimoníková, D. Němečková, A. Karafiátová, M. Uwimana, Brigitte Swennen, Rony L. Doležel, Jaroslav Hřibová, E. |
| author_sort | Šimoníková, D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis “DH Pahang” to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae. |
| format | Journal Article |
| id | CGSpace106239 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1062392025-11-11T11:07:51Z Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.) Šimoníková, D. Němečková, A. Karafiátová, M. Uwimana, Brigitte Swennen, Rony L. Doležel, Jaroslav Hřibová, E. bananas chromosomes karyotypes musa dna genomes Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis “DH Pahang” to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae. 2019 2019-12-19T09:41:18Z 2019-12-19T09:41:18Z Journal Article https://hdl.handle.net/10568/106239 en Open Access application/pdf Frontiers Media Šimoníková, D., Němečková, A., Karafiátová, M., Uwimana, B., Swennen, R., Doležel, J. & Hřibová, E. (2019). Chromosome painting facilitates anchoring reference genome sequence to chromosomes in situ and integrated karyotyping in banana (Musa spp.). Frontiers in Plant Science, 10, 1-12. |
| spellingShingle | bananas chromosomes karyotypes musa dna genomes Šimoníková, D. Němečková, A. Karafiátová, M. Uwimana, Brigitte Swennen, Rony L. Doležel, Jaroslav Hřibová, E. Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.) |
| title | Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.) |
| title_full | Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.) |
| title_fullStr | Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.) |
| title_full_unstemmed | Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.) |
| title_short | Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.) |
| title_sort | chromosome painting facilitates anchoring reference genome sequence to chromosomes in situ and integrated karyotyping in banana musa spp |
| topic | bananas chromosomes karyotypes musa dna genomes |
| url | https://hdl.handle.net/10568/106239 |
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