Monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing
Aflatoxins pose significant food security and public health risks, decrease productivity and profitability of animal industries, and hamper trade. To minimize aflatoxin contamination in several crops, a biocontrol technology based on atoxigenic strains of Aspergillus flavus is commercially used in t...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2019
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/105933 |
| _version_ | 1855532472658296832 |
|---|---|
| author | Shenge, K.C. Adhikari, B.N. Akande, A. Callicott, K.A. Atehnkeng, J. Ortega Beltran, A. Kumar, P. Lava Bandyopadhyay, Ranajit Cotty, P.J. |
| author_browse | Adhikari, B.N. Akande, A. Atehnkeng, J. Bandyopadhyay, Ranajit Callicott, K.A. Cotty, P.J. Kumar, P. Lava Ortega Beltran, A. Shenge, K.C. |
| author_facet | Shenge, K.C. Adhikari, B.N. Akande, A. Callicott, K.A. Atehnkeng, J. Ortega Beltran, A. Kumar, P. Lava Bandyopadhyay, Ranajit Cotty, P.J. |
| author_sort | Shenge, K.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Aflatoxins pose significant food security and public health risks, decrease productivity and profitability of animal industries, and hamper trade. To minimize aflatoxin contamination in several crops, a biocontrol technology based on atoxigenic strains of Aspergillus flavus is commercially used in the United States and some African countries. Significant efforts are underway to popularize the use of biocontrol in Africa by various means including incentives. The purpose of this study was to develop quantitative pyrosequencing assays for rapid, simultaneous quantification of proportions of four A. flavus biocontrol genotypes within complex populations of A. flavus associated with maize crops in Nigeria to facilitate payment of farmer incentives for Aflasafe (a biocontrol product) use. Protocols were developed to confirm use of Aflasafe by small scale farmers in Nigeria. Nested PCR amplifications followed by sequence by synthesis pyrosequencing assays were required to quantify frequencies of the active ingredients and, in so doing, confirm successful use of biocontrol by participating farmers. The entire verification process could be completed in 3–4 days proving a savings over other monitoring methods in both time and costs and providing data in a time frame that could work with the commercial agriculture scheme. Quantitative pyrosequencing assays represent a reliable tool for rapid detection, quantification, and monitoring of multiple A. flavus genotypes within complex fungal communities, satisfying the requirements of the regulatory community and crop end-users that wish to determine which purchased crops were treated with the biocontrol product. Techniques developed in the current study can be modified for monitoring other crop-associated fungi. |
| format | Journal Article |
| id | CGSpace105933 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1059332025-11-11T10:38:02Z Monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing Shenge, K.C. Adhikari, B.N. Akande, A. Callicott, K.A. Atehnkeng, J. Ortega Beltran, A. Kumar, P. Lava Bandyopadhyay, Ranajit Cotty, P.J. aflatoxins biological control monitoring maize nigeria aspergillus flavus genotypes Aflatoxins pose significant food security and public health risks, decrease productivity and profitability of animal industries, and hamper trade. To minimize aflatoxin contamination in several crops, a biocontrol technology based on atoxigenic strains of Aspergillus flavus is commercially used in the United States and some African countries. Significant efforts are underway to popularize the use of biocontrol in Africa by various means including incentives. The purpose of this study was to develop quantitative pyrosequencing assays for rapid, simultaneous quantification of proportions of four A. flavus biocontrol genotypes within complex populations of A. flavus associated with maize crops in Nigeria to facilitate payment of farmer incentives for Aflasafe (a biocontrol product) use. Protocols were developed to confirm use of Aflasafe by small scale farmers in Nigeria. Nested PCR amplifications followed by sequence by synthesis pyrosequencing assays were required to quantify frequencies of the active ingredients and, in so doing, confirm successful use of biocontrol by participating farmers. The entire verification process could be completed in 3–4 days proving a savings over other monitoring methods in both time and costs and providing data in a time frame that could work with the commercial agriculture scheme. Quantitative pyrosequencing assays represent a reliable tool for rapid detection, quantification, and monitoring of multiple A. flavus genotypes within complex fungal communities, satisfying the requirements of the regulatory community and crop end-users that wish to determine which purchased crops were treated with the biocontrol product. Techniques developed in the current study can be modified for monitoring other crop-associated fungi. 2019 2019-11-29T14:14:30Z 2019-11-29T14:14:30Z Journal Article https://hdl.handle.net/10568/105933 en Open Access application/pdf Frontiers Media Shenge, K.C., Adhikari, B.N., Akande, A., Callicott, K.A., Atehnkeng, J., Ortega-Beltran, A., ... & Cotty, P. J. (2019). Monitoring A. flavus with pyrosequencing monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing. Frontiers in Microbiology, 10, 1-13. |
| spellingShingle | aflatoxins biological control monitoring maize nigeria aspergillus flavus genotypes Shenge, K.C. Adhikari, B.N. Akande, A. Callicott, K.A. Atehnkeng, J. Ortega Beltran, A. Kumar, P. Lava Bandyopadhyay, Ranajit Cotty, P.J. Monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing |
| title | Monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing |
| title_full | Monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing |
| title_fullStr | Monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing |
| title_full_unstemmed | Monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing |
| title_short | Monitoring Aspergillus flavus genotypes in a multi-genotype aflatoxin biocontrol product with quantitative pyrosequencing |
| title_sort | monitoring aspergillus flavus genotypes in a multi genotype aflatoxin biocontrol product with quantitative pyrosequencing |
| topic | aflatoxins biological control monitoring maize nigeria aspergillus flavus genotypes |
| url | https://hdl.handle.net/10568/105933 |
| work_keys_str_mv | AT shengekc monitoringaspergillusflavusgenotypesinamultigenotypeaflatoxinbiocontrolproductwithquantitativepyrosequencing AT adhikaribn monitoringaspergillusflavusgenotypesinamultigenotypeaflatoxinbiocontrolproductwithquantitativepyrosequencing AT akandea monitoringaspergillusflavusgenotypesinamultigenotypeaflatoxinbiocontrolproductwithquantitativepyrosequencing AT callicottka monitoringaspergillusflavusgenotypesinamultigenotypeaflatoxinbiocontrolproductwithquantitativepyrosequencing AT atehnkengj monitoringaspergillusflavusgenotypesinamultigenotypeaflatoxinbiocontrolproductwithquantitativepyrosequencing AT ortegabeltrana monitoringaspergillusflavusgenotypesinamultigenotypeaflatoxinbiocontrolproductwithquantitativepyrosequencing AT kumarplava monitoringaspergillusflavusgenotypesinamultigenotypeaflatoxinbiocontrolproductwithquantitativepyrosequencing AT bandyopadhyayranajit monitoringaspergillusflavusgenotypesinamultigenotypeaflatoxinbiocontrolproductwithquantitativepyrosequencing AT cottypj monitoringaspergillusflavusgenotypesinamultigenotypeaflatoxinbiocontrolproductwithquantitativepyrosequencing |