A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat
The Ethiopian plateau hosts thousands of durum wheat (Triticum turgidum subsp. durum) farmer varieties (FV) with high adaptability and breeding potential. To harness their unique allelic diversity, we produced a large nested association mapping (NAM) population intercrossing fifty Ethiopian FVs with...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2019
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/105890 |
| _version_ | 1855538967116513280 |
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| author | Kidane, Yosef Gebrehawaryat Gesesse, C.A. Hailemariam, Bogale Nigir Desta, E.A. Mengistu, D.K. Fadda, Carlo Pe, M.E. Dell'Acqua, M. |
| author_browse | Dell'Acqua, M. Desta, E.A. Fadda, Carlo Gesesse, C.A. Hailemariam, Bogale Nigir Kidane, Yosef Gebrehawaryat Mengistu, D.K. Pe, M.E. |
| author_facet | Kidane, Yosef Gebrehawaryat Gesesse, C.A. Hailemariam, Bogale Nigir Desta, E.A. Mengistu, D.K. Fadda, Carlo Pe, M.E. Dell'Acqua, M. |
| author_sort | Kidane, Yosef Gebrehawaryat |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The Ethiopian plateau hosts thousands of durum wheat (Triticum turgidum subsp. durum) farmer varieties (FV) with high adaptability and breeding potential. To harness their unique allelic diversity, we produced a large nested association mapping (NAM) population intercrossing fifty Ethiopian FVs with an international elite durum wheat variety (Asassa). The Ethiopian NAM population (EtNAM) is composed of fifty interconnected bi-parental families, totalling 6280 recombinant inbred lines (RILs) that represent both a powerful quantitative trait loci (QTL) mapping tool, and a large pre-breeding panel. Here, we discuss the molecular and phenotypic diversity of the EtNAM founder lines, then we use an array featuring 13 000 single nucleotide polymorphisms (SNPs) to characterize a subset of 1200 EtNAM RILs from 12 families. Finally, we test the usefulness of the population by mapping phenology traits and plant height using a genome wide association (GWA) approach. EtNAM RILs showed high allelic variation and a genetic makeup combining genetic diversity from Ethiopian FVs with the international durum wheat allele pool. EtNAM SNP data were projected on the fully sequenced AB genome of wild emmer wheat, and were used to estimate pairwise linkage disequilibrium (LD) measures that reported an LD decay distance of 7.4 Mb on average, and balanced founder contributions across EtNAM families. GWA analyses identified 11 genomic loci individually affecting up to 3 days in flowering time and more than 1.6 cm in height. We argue that the EtNAM is a powerful tool to support the production of new durum wheat varieties targeting local and global agriculture. |
| format | Journal Article |
| id | CGSpace105890 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1058902025-11-12T05:48:12Z A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat Kidane, Yosef Gebrehawaryat Gesesse, C.A. Hailemariam, Bogale Nigir Desta, E.A. Mengistu, D.K. Fadda, Carlo Pe, M.E. Dell'Acqua, M. quantitative trait loci plant breeding smallholders tetraploidy wheat genomes The Ethiopian plateau hosts thousands of durum wheat (Triticum turgidum subsp. durum) farmer varieties (FV) with high adaptability and breeding potential. To harness their unique allelic diversity, we produced a large nested association mapping (NAM) population intercrossing fifty Ethiopian FVs with an international elite durum wheat variety (Asassa). The Ethiopian NAM population (EtNAM) is composed of fifty interconnected bi-parental families, totalling 6280 recombinant inbred lines (RILs) that represent both a powerful quantitative trait loci (QTL) mapping tool, and a large pre-breeding panel. Here, we discuss the molecular and phenotypic diversity of the EtNAM founder lines, then we use an array featuring 13 000 single nucleotide polymorphisms (SNPs) to characterize a subset of 1200 EtNAM RILs from 12 families. Finally, we test the usefulness of the population by mapping phenology traits and plant height using a genome wide association (GWA) approach. EtNAM RILs showed high allelic variation and a genetic makeup combining genetic diversity from Ethiopian FVs with the international durum wheat allele pool. EtNAM SNP data were projected on the fully sequenced AB genome of wild emmer wheat, and were used to estimate pairwise linkage disequilibrium (LD) measures that reported an LD decay distance of 7.4 Mb on average, and balanced founder contributions across EtNAM families. GWA analyses identified 11 genomic loci individually affecting up to 3 days in flowering time and more than 1.6 cm in height. We argue that the EtNAM is a powerful tool to support the production of new durum wheat varieties targeting local and global agriculture. 2019-07 2019-11-26T10:16:36Z 2019-11-26T10:16:36Z Journal Article https://hdl.handle.net/10568/105890 en Open Access application/pdf Wiley Kidane, Y.G.; Gesesse, C.A.; Hailemariam, B.N.; Desta, E.A.; Mengistu, D.K.; Fadda, C.; Pe, M.E.; Dell'Acqua, M. (2019). A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat. Plant Biotechnology Journal 17(7) p. 1380–1393. ISSN: 1467-7644 |
| spellingShingle | quantitative trait loci plant breeding smallholders tetraploidy wheat genomes Kidane, Yosef Gebrehawaryat Gesesse, C.A. Hailemariam, Bogale Nigir Desta, E.A. Mengistu, D.K. Fadda, Carlo Pe, M.E. Dell'Acqua, M. A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat |
| title | A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat |
| title_full | A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat |
| title_fullStr | A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat |
| title_full_unstemmed | A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat |
| title_short | A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat |
| title_sort | large nested association mapping population for breeding and quantitative trait locus mapping in ethiopian durum wheat |
| topic | quantitative trait loci plant breeding smallholders tetraploidy wheat genomes |
| url | https://hdl.handle.net/10568/105890 |
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