Genetic diversity of South African sweetpotato germplasm using molecular markers
Sweetpotato (Ipomoea batatas L.(LAM)) is one of the most important food crops in developing countries, including South Africa. Most sweetpotato varieties in South Africa are cream or white fleshed and lack the essential beta-carotene of the orange-fleshed varieties. To develop new varieties with hig...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Informa UK Limited
2019
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/105815 |
| _version_ | 1855513108249837568 |
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| author | Selaocoe, M.E. Adebola, P.O. Pillay, M. Laurie, S. |
| author_browse | Adebola, P.O. Laurie, S. Pillay, M. Selaocoe, M.E. |
| author_facet | Selaocoe, M.E. Adebola, P.O. Pillay, M. Laurie, S. |
| author_sort | Selaocoe, M.E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Sweetpotato (Ipomoea batatas L.(LAM)) is one of the most important food crops in developing countries, including South Africa. Most sweetpotato varieties in South Africa are cream or white fleshed and lack the essential beta-carotene of the orange-fleshed varieties. To develop new varieties with high beta-carotene, knowledge of the genetic variation of the crop is needed. The objectives of this study were to assess the genetic relationships among 30 orange- and cream-fleshed sweetpotato accessions by (i) examining the variation in leaf proteins and (ii) using random amplified polymorphic DNA (RAPD). The polymorphism levels of the proteins and RAPD assays were 55.6% and 98%, respectively. The dendrograms generated from all the analyses clustered the accessions according to their flesh color and country of origin. Analysis of molecular variance (AMOVA) found a significant difference between the South African and non-South African germplasm. The high genetic diversity in the South African sweetpotato germplasm is a positive indicator for a breeding program that has a number of targets, such as breeding for nutritional improvement, disease resistance and drought tolerance. |
| format | Journal Article |
| id | CGSpace105815 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace1058152023-10-02T11:39:31Z Genetic diversity of South African sweetpotato germplasm using molecular markers Selaocoe, M.E. Adebola, P.O. Pillay, M. Laurie, S. proteins rapd sweet potatoes molecular markers genetic markers germplasm genetic variation genetics Sweetpotato (Ipomoea batatas L.(LAM)) is one of the most important food crops in developing countries, including South Africa. Most sweetpotato varieties in South Africa are cream or white fleshed and lack the essential beta-carotene of the orange-fleshed varieties. To develop new varieties with high beta-carotene, knowledge of the genetic variation of the crop is needed. The objectives of this study were to assess the genetic relationships among 30 orange- and cream-fleshed sweetpotato accessions by (i) examining the variation in leaf proteins and (ii) using random amplified polymorphic DNA (RAPD). The polymorphism levels of the proteins and RAPD assays were 55.6% and 98%, respectively. The dendrograms generated from all the analyses clustered the accessions according to their flesh color and country of origin. Analysis of molecular variance (AMOVA) found a significant difference between the South African and non-South African germplasm. The high genetic diversity in the South African sweetpotato germplasm is a positive indicator for a breeding program that has a number of targets, such as breeding for nutritional improvement, disease resistance and drought tolerance. 2019-11-02 2019-11-19T15:25:54Z 2019-11-19T15:25:54Z Journal Article https://hdl.handle.net/10568/105815 en Limited Access Informa UK Limited Selaocoe, M.E., Adebola, P., Pillay, M. & Laurie, S.M. (2019). Genetic diversity of South African sweetpotato germplasm using molecular markers. Journal of Crop Improvement, 1-20. |
| spellingShingle | proteins rapd sweet potatoes molecular markers genetic markers germplasm genetic variation genetics Selaocoe, M.E. Adebola, P.O. Pillay, M. Laurie, S. Genetic diversity of South African sweetpotato germplasm using molecular markers |
| title | Genetic diversity of South African sweetpotato germplasm using molecular markers |
| title_full | Genetic diversity of South African sweetpotato germplasm using molecular markers |
| title_fullStr | Genetic diversity of South African sweetpotato germplasm using molecular markers |
| title_full_unstemmed | Genetic diversity of South African sweetpotato germplasm using molecular markers |
| title_short | Genetic diversity of South African sweetpotato germplasm using molecular markers |
| title_sort | genetic diversity of south african sweetpotato germplasm using molecular markers |
| topic | proteins rapd sweet potatoes molecular markers genetic markers germplasm genetic variation genetics |
| url | https://hdl.handle.net/10568/105815 |
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