Fruit set and plant regeneration in cassava following interspecific pollination with castor bean
The increasing demand for cassava (Manihot esculenta Crantz) for food and non-food uses in the tropics necessitates that its breeding for increased root productivity be made faster. The characteristic long breeding cycle and heterozygous nature of this crop, pose a major obstacle to its rapid geneti...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
African Journals Online
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/105509 |
| _version_ | 1855526811749842944 |
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| author | Baguma, J.K. Mukasa, S.B. Kawuki, R.S. Tugume, Arthur K. Buttibwa, M. Nalela, P. Eyokia, M. Oshaba, B. Ceballos, Hernán Lentini, Zaida Baguma, Y. |
| author_browse | Baguma, J.K. Baguma, Y. Buttibwa, M. Ceballos, Hernán Eyokia, M. Kawuki, R.S. Lentini, Zaida Mukasa, S.B. Nalela, P. Oshaba, B. Tugume, Arthur K. |
| author_facet | Baguma, J.K. Mukasa, S.B. Kawuki, R.S. Tugume, Arthur K. Buttibwa, M. Nalela, P. Eyokia, M. Oshaba, B. Ceballos, Hernán Lentini, Zaida Baguma, Y. |
| author_sort | Baguma, J.K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The increasing demand for cassava (Manihot esculenta Crantz) for food and non-food uses in the tropics necessitates that its breeding for increased root productivity be made faster. The characteristic long breeding cycle and heterozygous nature of this crop, pose a major obstacle to its rapid genetic improvement. This study aimed at inter-pollinating cassava with castor bean (Ricinus communis), with a purpose of inducing and regenerating cassava doubled haploids (DHs). A total of 3,349 flowers from twelve elite cassava varieties were inter-pollinated with caster bean. A total of 803 fruits were harvested for early embryo rescue and/or ovule culture. Of these, three were dissected to obtain seven unique embryos, while 800 were dissected to obtain 1312 young ovules, all of which were cultured in vitro. Overall, 82 (6.25%) of the cultured ovules formed callus that originated from the embryosac region, which is haploid. Four out of seven rescued embryos (57.1%) regenerated into plantlets. Ploidy analyses of 24 samples using flow cytometry revealed that 23 of the analysed samples were diploid. However, one callus sample was anueploid. Only one sample had an exceptionally high level of homozygosity ( 84.2%). These findings lay a foundation for future research aimed at induction of haploids in cassava. |
| format | Journal Article |
| id | CGSpace105509 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | African Journals Online |
| publisherStr | African Journals Online |
| record_format | dspace |
| spelling | CGSpace1055092025-03-13T09:44:21Z Fruit set and plant regeneration in cassava following interspecific pollination with castor bean Baguma, J.K. Mukasa, S.B. Kawuki, R.S. Tugume, Arthur K. Buttibwa, M. Nalela, P. Eyokia, M. Oshaba, B. Ceballos, Hernán Lentini, Zaida Baguma, Y. cassava manihot esculenta plant breeding varieties ploidy ploidía The increasing demand for cassava (Manihot esculenta Crantz) for food and non-food uses in the tropics necessitates that its breeding for increased root productivity be made faster. The characteristic long breeding cycle and heterozygous nature of this crop, pose a major obstacle to its rapid genetic improvement. This study aimed at inter-pollinating cassava with castor bean (Ricinus communis), with a purpose of inducing and regenerating cassava doubled haploids (DHs). A total of 3,349 flowers from twelve elite cassava varieties were inter-pollinated with caster bean. A total of 803 fruits were harvested for early embryo rescue and/or ovule culture. Of these, three were dissected to obtain seven unique embryos, while 800 were dissected to obtain 1312 young ovules, all of which were cultured in vitro. Overall, 82 (6.25%) of the cultured ovules formed callus that originated from the embryosac region, which is haploid. Four out of seven rescued embryos (57.1%) regenerated into plantlets. Ploidy analyses of 24 samples using flow cytometry revealed that 23 of the analysed samples were diploid. However, one callus sample was anueploid. Only one sample had an exceptionally high level of homozygosity ( 84.2%). These findings lay a foundation for future research aimed at induction of haploids in cassava. 2019 2019-10-24T16:42:23Z 2019-10-24T16:42:23Z Journal Article https://hdl.handle.net/10568/105509 en Open Access African Journals Online Baguma, J.K.; Mukasa, S.B. ; Kawuki, R.; Tugume, A.K. ; Buttibwa, M. ; Nalela, P. ; Eyokia, M. ; Oshaba, B. ; Ceballos, Hernan ; Lentini, Z. & Baguma, Y. (2019). Fruit set and plant regeneration in cassava following interspecific pollination with castor bean. African Crop Science Journal. 27(1): 99-118. |
| spellingShingle | cassava manihot esculenta plant breeding varieties ploidy ploidía Baguma, J.K. Mukasa, S.B. Kawuki, R.S. Tugume, Arthur K. Buttibwa, M. Nalela, P. Eyokia, M. Oshaba, B. Ceballos, Hernán Lentini, Zaida Baguma, Y. Fruit set and plant regeneration in cassava following interspecific pollination with castor bean |
| title | Fruit set and plant regeneration in cassava following interspecific pollination with castor bean |
| title_full | Fruit set and plant regeneration in cassava following interspecific pollination with castor bean |
| title_fullStr | Fruit set and plant regeneration in cassava following interspecific pollination with castor bean |
| title_full_unstemmed | Fruit set and plant regeneration in cassava following interspecific pollination with castor bean |
| title_short | Fruit set and plant regeneration in cassava following interspecific pollination with castor bean |
| title_sort | fruit set and plant regeneration in cassava following interspecific pollination with castor bean |
| topic | cassava manihot esculenta plant breeding varieties ploidy ploidía |
| url | https://hdl.handle.net/10568/105509 |
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