Cryopreservation of Musa germplasm
This publication describes cryopreservation methods developed for Musa tissue at KUL (Katholieke Universiteit Leuven, Belgium) and JIRCAS (Japanese International Research Centre for Agricultural Sciences). Detailed protocols are given for all steps to follow from the preparation of the plant materia...
| Autores principales: | , , |
|---|---|
| Formato: | Manual |
| Lenguaje: | Inglés Español |
| Publicado: |
2001
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/105372 |
| _version_ | 1855530884556390400 |
|---|---|
| author | Panis, Bartholomeus Thinh, N.T. International Network for the Improvement of Banana and Plantain |
| author_browse | International Network for the Improvement of Banana and Plantain Panis, Bartholomeus Thinh, N.T. |
| author_facet | Panis, Bartholomeus Thinh, N.T. International Network for the Improvement of Banana and Plantain |
| author_sort | Panis, Bartholomeus |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This publication describes cryopreservation methods developed for Musa tissue at KUL (Katholieke Universiteit Leuven, Belgium) and JIRCAS (Japanese International Research Centre for Agricultural Sciences). Detailed protocols are given for all steps to follow from the preparation of the plant material to the recovery and regeneration of whole plants. These methodologies are specific to the type of banana tissue to be cryopreserved: individual meristems, meristem clumps (cauliflower-like structures), embryogenic cell suspensions, and zygotic embryos. Each method is illustrated with figures and colour photographs. Advantages and limitations of each method are highlighted, and current perspectives for optimizing the methodologies presented are discussed. It is hoped that these guidelines will facilitate the adoption and use of the methodologies described. The publication includes bibliographical references and some useful practical information: composition of media and solutions, and list of the basic equipment needed. |
| format | Manual |
| id | CGSpace105372 |
| institution | CGIAR Consortium |
| language | Inglés Español |
| publishDate | 2001 |
| publishDateRange | 2001 |
| publishDateSort | 2001 |
| record_format | dspace |
| spelling | CGSpace1053722025-11-05T08:17:09Z Cryopreservation of Musa germplasm Panis, Bartholomeus Thinh, N.T. International Network for the Improvement of Banana and Plantain musa (bananas) plantains germplasm disease resistance virology biodiversity plant genetic resources This publication describes cryopreservation methods developed for Musa tissue at KUL (Katholieke Universiteit Leuven, Belgium) and JIRCAS (Japanese International Research Centre for Agricultural Sciences). Detailed protocols are given for all steps to follow from the preparation of the plant material to the recovery and regeneration of whole plants. These methodologies are specific to the type of banana tissue to be cryopreserved: individual meristems, meristem clumps (cauliflower-like structures), embryogenic cell suspensions, and zygotic embryos. Each method is illustrated with figures and colour photographs. Advantages and limitations of each method are highlighted, and current perspectives for optimizing the methodologies presented are discussed. It is hoped that these guidelines will facilitate the adoption and use of the methodologies described. The publication includes bibliographical references and some useful practical information: composition of media and solutions, and list of the basic equipment needed. 2001 2019-10-15T15:45:57Z 2019-10-15T15:45:57Z Manual https://hdl.handle.net/10568/105372 en es Open Access application/pdf application/pdf International Network for the Improvement of Banana and Plantain; Panis, B.; Thinh, N.T. (2001) Cryopreservation of Musa germplasm. INIBAP Technical Guidelines n. 5, 45 p. ISBN: 2-910810-45-3 |
| spellingShingle | musa (bananas) plantains germplasm disease resistance virology biodiversity plant genetic resources Panis, Bartholomeus Thinh, N.T. International Network for the Improvement of Banana and Plantain Cryopreservation of Musa germplasm |
| title | Cryopreservation of Musa germplasm |
| title_full | Cryopreservation of Musa germplasm |
| title_fullStr | Cryopreservation of Musa germplasm |
| title_full_unstemmed | Cryopreservation of Musa germplasm |
| title_short | Cryopreservation of Musa germplasm |
| title_sort | cryopreservation of musa germplasm |
| topic | musa (bananas) plantains germplasm disease resistance virology biodiversity plant genetic resources |
| url | https://hdl.handle.net/10568/105372 |
| work_keys_str_mv | AT panisbartholomeus cryopreservationofmusagermplasm AT thinhnt cryopreservationofmusagermplasm AT internationalnetworkfortheimprovementofbananaandplantain cryopreservationofmusagermplasm |