Characterisation of wild musa accessions recently introduced to the International Gene Bank

Bananas and plantains (Musa spp.) are one of the most important world trade commodities and are a staple food for millions of people in countries of the humid tropics. The production of bananas is, however, threatened by the rapid spread of various diseases and adverse environmental conditions. The...

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Main Authors: Cizkova, J., Hribova, E., Christelova, P., Houwe, Ines van den, Swennen, Rony L., Roux, N., Doležel, Jaroslav
Format: Conference Paper Abstract
Language:Inglés
Published: Tropentag 2014
Online Access:https://hdl.handle.net/10568/104492
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author Cizkova, J.
Hribova, E.
Christelova, P.
Houwe, Ines van den
Swennen, Rony L.
Roux, N.
Doležel, Jaroslav
author_browse Christelova, P.
Cizkova, J.
Doležel, Jaroslav
Houwe, Ines van den
Hribova, E.
Roux, N.
Swennen, Rony L.
author_facet Cizkova, J.
Hribova, E.
Christelova, P.
Houwe, Ines van den
Swennen, Rony L.
Roux, N.
Doležel, Jaroslav
author_sort Cizkova, J.
collection Repository of Agricultural Research Outputs (CGSpace)
description Bananas and plantains (Musa spp.) are one of the most important world trade commodities and are a staple food for millions of people in countries of the humid tropics. The production of bananas is, however, threatened by the rapid spread of various diseases and adverse environmental conditions. The Musa genetic diversity, which is of paramount importance for breeding of resistant cultivars, needs to be preserved and better characterised. The world's largest banana and plantain collection is managed by the Bioversity International Transit Centre (ITC) in Belgium and contains more than 1500 accessions maintained in vitro. The collection is being continuously expanded by new accessions representing various edible cultivars, improved materials and wild species from different parts of the world. Recently new germplasm was collected in Indonesia and successively introduced into the international Musa gene bank. The aim of this work was to characterise the genotype of these accessions in order to shed light on their genome structure and to confirm their taxonomic classification. A total of 21 wild Musa accessions were analysed and their nuclear genome size and the genomic distribution of ribosomal RNA genes were determined, showing a high degree of variability in both characters. Genotyping with a set of 19 microsatellite markers identified Musa species that are closely related to the studied accessions and provided data to aid in their classification. Sequence analysis of their internal transcribed spacers ITS1 and ITS2 suggested that some of the accessions are of interspecific hybrid origin and/or represent backcross progenies of interspecific hybrids.
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institution CGIAR Consortium
language Inglés
publishDate 2014
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spelling CGSpace1044922025-11-05T08:01:51Z Characterisation of wild musa accessions recently introduced to the International Gene Bank Cizkova, J. Hribova, E. Christelova, P. Houwe, Ines van den Swennen, Rony L. Roux, N. Doležel, Jaroslav Bananas and plantains (Musa spp.) are one of the most important world trade commodities and are a staple food for millions of people in countries of the humid tropics. The production of bananas is, however, threatened by the rapid spread of various diseases and adverse environmental conditions. The Musa genetic diversity, which is of paramount importance for breeding of resistant cultivars, needs to be preserved and better characterised. The world's largest banana and plantain collection is managed by the Bioversity International Transit Centre (ITC) in Belgium and contains more than 1500 accessions maintained in vitro. The collection is being continuously expanded by new accessions representing various edible cultivars, improved materials and wild species from different parts of the world. Recently new germplasm was collected in Indonesia and successively introduced into the international Musa gene bank. The aim of this work was to characterise the genotype of these accessions in order to shed light on their genome structure and to confirm their taxonomic classification. A total of 21 wild Musa accessions were analysed and their nuclear genome size and the genomic distribution of ribosomal RNA genes were determined, showing a high degree of variability in both characters. Genotyping with a set of 19 microsatellite markers identified Musa species that are closely related to the studied accessions and provided data to aid in their classification. Sequence analysis of their internal transcribed spacers ITS1 and ITS2 suggested that some of the accessions are of interspecific hybrid origin and/or represent backcross progenies of interspecific hybrids. 2014 2019-10-15T15:41:28Z 2019-10-15T15:41:28Z Conference Paper Abstract https://hdl.handle.net/10568/104492 en Open Access application/pdf Tropentag Cizkova, J.; Hribova, E.; Christelova, P.; van den Houwe, I.; Swennen, R.; Roux, N.; Dolezel, J. (2014) Characterisation of wild musa accessions recently introduced to the International Gene Bank. Tropentag 2014. International Conference on Research on Food Security, Natural Resource Management and Rural Development.
spellingShingle Cizkova, J.
Hribova, E.
Christelova, P.
Houwe, Ines van den
Swennen, Rony L.
Roux, N.
Doležel, Jaroslav
Characterisation of wild musa accessions recently introduced to the International Gene Bank
title Characterisation of wild musa accessions recently introduced to the International Gene Bank
title_full Characterisation of wild musa accessions recently introduced to the International Gene Bank
title_fullStr Characterisation of wild musa accessions recently introduced to the International Gene Bank
title_full_unstemmed Characterisation of wild musa accessions recently introduced to the International Gene Bank
title_short Characterisation of wild musa accessions recently introduced to the International Gene Bank
title_sort characterisation of wild musa accessions recently introduced to the international gene bank
url https://hdl.handle.net/10568/104492
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