AFLP Analysis of Gene Pools for a Wild Bean Core Collection

Studies of diversity permit conclusions about the genetic structure of species. Systematically developed core collections are a particularly good tool for such purposes. The objective of this study was to evaluate the genetic structure between and within gene pools of a core collection of wild Phase...

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Main Authors: Tohme, Joseph M., González, Delkin Orlando, Beebe, Stephen E., Duque E., Myriam Cristina
Format: Journal Article
Language:Inglés
Published: Wiley 1996
Online Access:https://hdl.handle.net/10568/103995
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author Tohme, Joseph M.
González, Delkin Orlando
Beebe, Stephen E.
Duque E., Myriam Cristina
author_browse Beebe, Stephen E.
Duque E., Myriam Cristina
González, Delkin Orlando
Tohme, Joseph M.
author_facet Tohme, Joseph M.
González, Delkin Orlando
Beebe, Stephen E.
Duque E., Myriam Cristina
author_sort Tohme, Joseph M.
collection Repository of Agricultural Research Outputs (CGSpace)
description Studies of diversity permit conclusions about the genetic structure of species. Systematically developed core collections are a particularly good tool for such purposes. The objective of this study was to evaluate the genetic structure between and within gene pools of a core collection of wild Phaseolus vulgaris L. using the technique of amplified restriction polymorphism (AFLP) analysis. DNA fingerprint patterns of 114 genotypes were analyzed by the NTSYS program. Major groups or gene pools were recognized in Mesoamerica, Colombia, the northern Andes of Ecuador and northern Peru, and the southern Andes, although the separation among gene pools was not wide. A unique segment of germplasm from northern Peru showed greater genetic distance than the other gene pools. Although most Mexican wild beans formed a rather homogenous group, a small number of accessions clustered with the Andean gene pool, and accessions from Guatemala tended to cluster apart from Mexican accessions. In the southern Andean gene pool, more discrete groups were formed which were associated with certain regions such as Argentina, possibly reflecting greater geographic and genetic isolation. Colombian accessions appeared to be highly introgressed with germplasm from other areas. AFLP analysis produced a large amount of data in a short period, thus permitting greater insights into the genetic structure of wild beans than had been possible with other methods of analysis.
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spelling CGSpace1039952025-03-13T09:44:55Z AFLP Analysis of Gene Pools for a Wild Bean Core Collection Tohme, Joseph M. González, Delkin Orlando Beebe, Stephen E. Duque E., Myriam Cristina Studies of diversity permit conclusions about the genetic structure of species. Systematically developed core collections are a particularly good tool for such purposes. The objective of this study was to evaluate the genetic structure between and within gene pools of a core collection of wild Phaseolus vulgaris L. using the technique of amplified restriction polymorphism (AFLP) analysis. DNA fingerprint patterns of 114 genotypes were analyzed by the NTSYS program. Major groups or gene pools were recognized in Mesoamerica, Colombia, the northern Andes of Ecuador and northern Peru, and the southern Andes, although the separation among gene pools was not wide. A unique segment of germplasm from northern Peru showed greater genetic distance than the other gene pools. Although most Mexican wild beans formed a rather homogenous group, a small number of accessions clustered with the Andean gene pool, and accessions from Guatemala tended to cluster apart from Mexican accessions. In the southern Andean gene pool, more discrete groups were formed which were associated with certain regions such as Argentina, possibly reflecting greater geographic and genetic isolation. Colombian accessions appeared to be highly introgressed with germplasm from other areas. AFLP analysis produced a large amount of data in a short period, thus permitting greater insights into the genetic structure of wild beans than had been possible with other methods of analysis. 1996-09 2019-10-08T16:49:40Z 2019-10-08T16:49:40Z Journal Article https://hdl.handle.net/10568/103995 en Limited Access Wiley Tohme, Joe; González, D.Orlando; Beebe, Stephen; Duque, Myrian C. (1997). AFLP Analysis of Gene Pools for a Wild Bean Core Collection. Crop Sciences. 36(5): 1375-1384
spellingShingle Tohme, Joseph M.
González, Delkin Orlando
Beebe, Stephen E.
Duque E., Myriam Cristina
AFLP Analysis of Gene Pools for a Wild Bean Core Collection
title AFLP Analysis of Gene Pools for a Wild Bean Core Collection
title_full AFLP Analysis of Gene Pools for a Wild Bean Core Collection
title_fullStr AFLP Analysis of Gene Pools for a Wild Bean Core Collection
title_full_unstemmed AFLP Analysis of Gene Pools for a Wild Bean Core Collection
title_short AFLP Analysis of Gene Pools for a Wild Bean Core Collection
title_sort aflp analysis of gene pools for a wild bean core collection
url https://hdl.handle.net/10568/103995
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