Association genetics of bunch weight and its component traits in East African highland banana (Musa spp. AAA group)

Bunch weight increase is one of the major objectives of banana improvement programs, but little is known about the loci controlling bunch weight and its component traits. Here we report for the first time some genomic loci associated with bunch weight and its component traits in banana as revealed t...

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Autores principales: Nyine, M., Uwimana, Brigitte, Akech, V., Brown, A., Ortíz, R., Doležel, Jaroslav, Lorenzen, J., Swennen, Rony L.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2019
Materias:
Acceso en línea:https://hdl.handle.net/10568/103733
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author Nyine, M.
Uwimana, Brigitte
Akech, V.
Brown, A.
Ortíz, R.
Doležel, Jaroslav
Lorenzen, J.
Swennen, Rony L.
author_browse Akech, V.
Brown, A.
Doležel, Jaroslav
Lorenzen, J.
Nyine, M.
Ortíz, R.
Swennen, Rony L.
Uwimana, Brigitte
author_facet Nyine, M.
Uwimana, Brigitte
Akech, V.
Brown, A.
Ortíz, R.
Doležel, Jaroslav
Lorenzen, J.
Swennen, Rony L.
author_sort Nyine, M.
collection Repository of Agricultural Research Outputs (CGSpace)
description Bunch weight increase is one of the major objectives of banana improvement programs, but little is known about the loci controlling bunch weight and its component traits. Here we report for the first time some genomic loci associated with bunch weight and its component traits in banana as revealed through a genome-wide association study. A banana-breeding population of 307 genotypes varying in ploidy was phenotyped in three locations under different environmental conditions, and data were collected on bunch weight, number of hands and fruits; fruit length and circumference; and diameter of both fruit and pulp for three crop cycles. The population was genotyped with genotyping by sequencing and 27,178 single nucleotide polymorphisms (SNPs) were generated. The association between SNPs and the best linear unbiased predictors of traits was performed with TASSEL v5 using a mixed linear model accounting for population structure and kinship. Using Bonferroni correction, false discovery rate, and long-range linkage disequilibrium (LD), 25 genomic loci were identified with significant SNPs and most were localized on chromosome 3. Most SNPs were located in genes encoding uncharacterized and hypothetical proteins, but some mapped to transcription factors and genes involved in cell cycle regulation. Inter-chromosomal LD of SNPs was present in the population, but none of the SNPs were significantly associated with the traits. The clustering of significant SNPs on chromosome 3 supported our hypothesis that fruit filling in this population was under control of a few quantitative trait loci with major effects.
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spelling CGSpace1037332025-11-12T05:44:11Z Association genetics of bunch weight and its component traits in East African highland banana (Musa spp. AAA group) Nyine, M. Uwimana, Brigitte Akech, V. Brown, A. Ortíz, R. Doležel, Jaroslav Lorenzen, J. Swennen, Rony L. musa genotypes phenotypes quantitative trait loci single nucleotide polymorphism agronomic characters Bunch weight increase is one of the major objectives of banana improvement programs, but little is known about the loci controlling bunch weight and its component traits. Here we report for the first time some genomic loci associated with bunch weight and its component traits in banana as revealed through a genome-wide association study. A banana-breeding population of 307 genotypes varying in ploidy was phenotyped in three locations under different environmental conditions, and data were collected on bunch weight, number of hands and fruits; fruit length and circumference; and diameter of both fruit and pulp for three crop cycles. The population was genotyped with genotyping by sequencing and 27,178 single nucleotide polymorphisms (SNPs) were generated. The association between SNPs and the best linear unbiased predictors of traits was performed with TASSEL v5 using a mixed linear model accounting for population structure and kinship. Using Bonferroni correction, false discovery rate, and long-range linkage disequilibrium (LD), 25 genomic loci were identified with significant SNPs and most were localized on chromosome 3. Most SNPs were located in genes encoding uncharacterized and hypothetical proteins, but some mapped to transcription factors and genes involved in cell cycle regulation. Inter-chromosomal LD of SNPs was present in the population, but none of the SNPs were significantly associated with the traits. The clustering of significant SNPs on chromosome 3 supported our hypothesis that fruit filling in this population was under control of a few quantitative trait loci with major effects. 2019-09-16 2019-09-24T07:58:31Z 2019-09-24T07:58:31Z Journal Article https://hdl.handle.net/10568/103733 en Open Access application/pdf Springer Nyine, M.; Uwimana, B.; Akech, V.; Brown, A.; Ortiz, R.; Doležel, J.; Lorenzen, J.; Swennen, R. (2019) Association genetics of bunch weight and its component traits in East African highland banana (Musa spp. AAA group). Theoretical and Applied Genetics 132, p. 3295–3308. ISSN: 1432-2242
spellingShingle musa
genotypes
phenotypes
quantitative trait loci
single nucleotide polymorphism
agronomic characters
Nyine, M.
Uwimana, Brigitte
Akech, V.
Brown, A.
Ortíz, R.
Doležel, Jaroslav
Lorenzen, J.
Swennen, Rony L.
Association genetics of bunch weight and its component traits in East African highland banana (Musa spp. AAA group)
title Association genetics of bunch weight and its component traits in East African highland banana (Musa spp. AAA group)
title_full Association genetics of bunch weight and its component traits in East African highland banana (Musa spp. AAA group)
title_fullStr Association genetics of bunch weight and its component traits in East African highland banana (Musa spp. AAA group)
title_full_unstemmed Association genetics of bunch weight and its component traits in East African highland banana (Musa spp. AAA group)
title_short Association genetics of bunch weight and its component traits in East African highland banana (Musa spp. AAA group)
title_sort association genetics of bunch weight and its component traits in east african highland banana musa spp aaa group
topic musa
genotypes
phenotypes
quantitative trait loci
single nucleotide polymorphism
agronomic characters
url https://hdl.handle.net/10568/103733
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