Age-dependent regulation of host seeking in Anopheles coluzzii

Behavioural attraction of the malaria vector Anopheles coluzzii to human host odour increases during adult maturation. We have previously demonstrated that the onset of host seeking in An. coluzzii coincides with an increased sensitivity of the CO2-sensitive neurons and abundance of chemosensory rec...

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Autores principales: Omondi, Bonaventure Aman Oduor, Ghaninia, M., Dawit, M., Svensson, T., Ignell, R.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2019
Materias:
Acceso en línea:https://hdl.handle.net/10568/103676
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author Omondi, Bonaventure Aman Oduor
Ghaninia, M.
Dawit, M.
Svensson, T.
Ignell, R.
author_browse Dawit, M.
Ghaninia, M.
Ignell, R.
Omondi, Bonaventure Aman Oduor
Svensson, T.
author_facet Omondi, Bonaventure Aman Oduor
Ghaninia, M.
Dawit, M.
Svensson, T.
Ignell, R.
author_sort Omondi, Bonaventure Aman Oduor
collection Repository of Agricultural Research Outputs (CGSpace)
description Behavioural attraction of the malaria vector Anopheles coluzzii to human host odour increases during adult maturation. We have previously demonstrated that the onset of host seeking in An. coluzzii coincides with an increased sensitivity of the CO2-sensitive neurons and abundance of chemosensory receptor gene transcripts in the maxillary palp. In this study, we extend our analysis to the antenna. Functional characterisation of the near-complete repertoire of odorant receptors (Ors) expressed in this tissue, to fractioned human odour, reveals a subset of salient human odorants to be detected by Ors at physiological relevant concentrations. When presented as a blend in their ratio of natural emission, these odorants elicit attraction by host-seeking mosquitoes, emphasising that Ors alone can mediate this behaviour. However, the same blend inhibits attraction in teneral mosquitoes. This switch in behavioural response indicates a change in valence during adult maturation. Quantitative analysis of Or transcript abundance and in vivo electrophysiological analysis reveal that the olfactory system of female An. coluzzii undergoes concerted changes that correlate with the onset of host seeking. We conclude that changes in Or abundance modulate peripheral olfactory coding, resulting in ecologically relevant behavioural effects.
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spelling CGSpace1036762025-11-12T05:44:51Z Age-dependent regulation of host seeking in Anopheles coluzzii Omondi, Bonaventure Aman Oduor Ghaninia, M. Dawit, M. Svensson, T. Ignell, R. culicidae anopheles olfaction smell receptors maturity Behavioural attraction of the malaria vector Anopheles coluzzii to human host odour increases during adult maturation. We have previously demonstrated that the onset of host seeking in An. coluzzii coincides with an increased sensitivity of the CO2-sensitive neurons and abundance of chemosensory receptor gene transcripts in the maxillary palp. In this study, we extend our analysis to the antenna. Functional characterisation of the near-complete repertoire of odorant receptors (Ors) expressed in this tissue, to fractioned human odour, reveals a subset of salient human odorants to be detected by Ors at physiological relevant concentrations. When presented as a blend in their ratio of natural emission, these odorants elicit attraction by host-seeking mosquitoes, emphasising that Ors alone can mediate this behaviour. However, the same blend inhibits attraction in teneral mosquitoes. This switch in behavioural response indicates a change in valence during adult maturation. Quantitative analysis of Or transcript abundance and in vivo electrophysiological analysis reveal that the olfactory system of female An. coluzzii undergoes concerted changes that correlate with the onset of host seeking. We conclude that changes in Or abundance modulate peripheral olfactory coding, resulting in ecologically relevant behavioural effects. 2019 2019-09-18T10:42:35Z 2019-09-18T10:42:35Z Journal Article https://hdl.handle.net/10568/103676 en Open Access application/pdf Springer Omondi, A.B.; Ghaninia, M.; Dawit, M.; Svensson, T.; Ignell, R. (2019) Age-dependent regulation of host seeking in Anopheles coluzzii. Scientific Reports 9(1), Article no. 9699. ISSN: 2045-2322
spellingShingle culicidae
anopheles
olfaction
smell
receptors
maturity
Omondi, Bonaventure Aman Oduor
Ghaninia, M.
Dawit, M.
Svensson, T.
Ignell, R.
Age-dependent regulation of host seeking in Anopheles coluzzii
title Age-dependent regulation of host seeking in Anopheles coluzzii
title_full Age-dependent regulation of host seeking in Anopheles coluzzii
title_fullStr Age-dependent regulation of host seeking in Anopheles coluzzii
title_full_unstemmed Age-dependent regulation of host seeking in Anopheles coluzzii
title_short Age-dependent regulation of host seeking in Anopheles coluzzii
title_sort age dependent regulation of host seeking in anopheles coluzzii
topic culicidae
anopheles
olfaction
smell
receptors
maturity
url https://hdl.handle.net/10568/103676
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AT svenssont agedependentregulationofhostseekinginanophelescoluzzii
AT ignellr agedependentregulationofhostseekinginanophelescoluzzii