A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.
A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced by...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2002
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| Acceso en línea: | https://hdl.handle.net/10568/103631 |
| _version_ | 1855535175675412480 |
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| author | Njukeng, A.P. Ariri, G.I. Hughes, Jacqueline d'Arros Agindoran, B.O. Mignouna, Hodeba D. Thottappilly, G. |
| author_browse | Agindoran, B.O. Ariri, G.I. Hughes, Jacqueline d'Arros Mignouna, Hodeba D. Njukeng, A.P. Thottappilly, G. |
| author_facet | Njukeng, A.P. Ariri, G.I. Hughes, Jacqueline d'Arros Agindoran, B.O. Mignouna, Hodeba D. Thottappilly, G. |
| author_sort | Njukeng, A.P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced by fusion of FOX-NY myeloma cells with immune splenocytes from in vivo immunizations of a Balb/C mouse. The polyclonal antiserum to YMV and the Mab (YMV-M24) were used in developing a triple antibody sandwich (TAS) enzyme-linked immunosorbent assay (ELISA) system for the detection of YMV from samples collected from fields. The test gave a positive reaction even at a sap dilution of 1:6250-1:31250 from leaf samples collected from different plants species, while antigen-coated plant (ACP) ELISA using the Mab could detect only to a dilution of 1:1250. TAS-ELISA was more sensitive and reliable when compared with ACP-ELISA using YMV-M24. YMV-M24 detected YMV isolates in some Dioscorea species from seven countries in the main yam growing area of West Africa. |
| format | Journal Article |
| id | CGSpace103631 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| record_format | dspace |
| spelling | CGSpace1036312024-03-06T10:16:43Z A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. Njukeng, A.P. Ariri, G.I. Hughes, Jacqueline d'Arros Agindoran, B.O. Mignouna, Hodeba D. Thottappilly, G. yams plant viruses monoclonal antibodies dioscorea A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced by fusion of FOX-NY myeloma cells with immune splenocytes from in vivo immunizations of a Balb/C mouse. The polyclonal antiserum to YMV and the Mab (YMV-M24) were used in developing a triple antibody sandwich (TAS) enzyme-linked immunosorbent assay (ELISA) system for the detection of YMV from samples collected from fields. The test gave a positive reaction even at a sap dilution of 1:6250-1:31250 from leaf samples collected from different plants species, while antigen-coated plant (ACP) ELISA using the Mab could detect only to a dilution of 1:1250. TAS-ELISA was more sensitive and reliable when compared with ACP-ELISA using YMV-M24. YMV-M24 detected YMV isolates in some Dioscorea species from seven countries in the main yam growing area of West Africa. 2002 2019-09-12T08:32:00Z 2019-09-12T08:32:00Z Journal Article https://hdl.handle.net/10568/103631 en Limited Access Njukeng, A.P., Atiri, G.I., D'A Hughes, J., Agindotan, B.O., Mignouna, H.D. & Thottappilly, G. (2002). A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. Tropical Science, 42(2), 65-74. |
| spellingShingle | yams plant viruses monoclonal antibodies dioscorea Njukeng, A.P. Ariri, G.I. Hughes, Jacqueline d'Arros Agindoran, B.O. Mignouna, Hodeba D. Thottappilly, G. A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. |
| title | A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. |
| title_full | A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. |
| title_fullStr | A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. |
| title_full_unstemmed | A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. |
| title_short | A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. |
| title_sort | sensitive tas elisa for the detection of some west african isolates of yam mosaic virus in dioscorea spp |
| topic | yams plant viruses monoclonal antibodies dioscorea |
| url | https://hdl.handle.net/10568/103631 |
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