Isolation of phytoplasmas associated to frogskin disease in cassava
Frogskin is the most limiting disease of cassava crops in Colombia, causing losses in production up to 90%. Since this disease was associatated with 16SrIII phytoplasma presence, a study was carried out to isolate this phytoplasma using liquid and solid culture media. Root, petiol, stem, leaf and cu...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Universidad de Ciencias Aplicadas Y Ambientales - UDCA
2019
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| Acceso en línea: | https://hdl.handle.net/10568/103612 |
| _version_ | 1855525421167149056 |
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| author | Betancourth, Carlos Pardo, Juan Manuel Muñoz, Jaime E. Álvarez, Elizabeth |
| author_browse | Betancourth, Carlos Muñoz, Jaime E. Pardo, Juan Manuel Álvarez, Elizabeth |
| author_facet | Betancourth, Carlos Pardo, Juan Manuel Muñoz, Jaime E. Álvarez, Elizabeth |
| author_sort | Betancourth, Carlos |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Frogskin is the most limiting disease of cassava crops in Colombia, causing losses in production up to 90%. Since this disease was associatated with 16SrIII phytoplasma presence, a study was carried out to isolate this phytoplasma using liquid and solid culture media. Root, petiol, stem, leaf and cutting tissues of cassava affected by frogsking were employed as source materials. Molecular and microscopy techniques were applied to verify the phytoplasma growth and to discard other microorganism ́s presence. The results showed that the media consistently allow phytoplasma growth, and colonies in solid medium were obtained. PCR, qPCR and sequencing tests confi rmed the presence of 16SrIII group phytoplasmas in both liquid and solid culture media. Additionally, the isolation of a pigeon pea witches' broom phytoplasma strain (group 16SrIX) was obtained from stems, petioles and fl owers of symptomatic Catharanthus roseus confi rming the effectiveness of the medium in the phytoplasma isolation and culture. This is the fi rst isolation of fi eld-collected phytoplasma strains in groups 16SrIII and 16SrIX in America that confi rm and corroborate the previous results in phytoplasma cultivation achieved on micropropagated and fi eld-collected phytoplasma infected samples. |
| format | Journal Article |
| id | CGSpace103612 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Universidad de Ciencias Aplicadas Y Ambientales - UDCA |
| publisherStr | Universidad de Ciencias Aplicadas Y Ambientales - UDCA |
| record_format | dspace |
| spelling | CGSpace1036122025-05-29T08:57:03Z Isolation of phytoplasmas associated to frogskin disease in cassava Aislamiento de fi toplasmas asociados a cuero de sapo en yuca Betancourth, Carlos Pardo, Juan Manuel Muñoz, Jaime E. Álvarez, Elizabeth cassava pests of plants aetiology etiología cultivos industriales industrial crops Frogskin is the most limiting disease of cassava crops in Colombia, causing losses in production up to 90%. Since this disease was associatated with 16SrIII phytoplasma presence, a study was carried out to isolate this phytoplasma using liquid and solid culture media. Root, petiol, stem, leaf and cutting tissues of cassava affected by frogsking were employed as source materials. Molecular and microscopy techniques were applied to verify the phytoplasma growth and to discard other microorganism ́s presence. The results showed that the media consistently allow phytoplasma growth, and colonies in solid medium were obtained. PCR, qPCR and sequencing tests confi rmed the presence of 16SrIII group phytoplasmas in both liquid and solid culture media. Additionally, the isolation of a pigeon pea witches' broom phytoplasma strain (group 16SrIX) was obtained from stems, petioles and fl owers of symptomatic Catharanthus roseus confi rming the effectiveness of the medium in the phytoplasma isolation and culture. This is the fi rst isolation of fi eld-collected phytoplasma strains in groups 16SrIII and 16SrIX in America that confi rm and corroborate the previous results in phytoplasma cultivation achieved on micropropagated and fi eld-collected phytoplasma infected samples. En Colombia, el "cuero de sapo" es la enfermedad más limitante del cultivo de yuca, que ocasiona pérdidas en producción de raíces hasta del 90%. La presente investigación tuvo como objetivo, el aislamiento in vitro del fitoplasma asociado a cuero de sapo. Para ello, se emplearon medios de cultivo líquido y sólido, usando tejidos de raíces, peciolos, tallos, hojas y semillas de yuca, afectada por la enfermedad. Pruebas de PCR, qPCR, secuenciación, microscopia de luz y microscopia electrónica de transmisión fueron aplicadas, para verificar el crecimiento de fitoplasmas y descartar la presencia de otros microrganismos. Los resultados muestran que los medios permiten, consistentemente, el crecimiento de fitoplasmas, obteniendo colonias en medio sólido a partir de medio líquido. Las pruebas de PCR, qPCR y secuenciación confirmaron presencia de Cassava frogskin phytoplasma del grupo 16SrIII, en los dos medios de cultivo. Además, a partir de las colonias, se lograron fotografías de células con morfología y tamaño similares a las fitoplasmáticas. Es la primera vez, en el mundo, que se consolida información suficiente del aislamiento de fitoplasmas en medio artificial. Adicionalmente, se logró el aislamiento de Pigeon pea witches´ broom phytoplasma del grupo IX, a partir de tallos, peciolos y flores de vinca (Catharanthus roseus), con síntomas asociados a fitoplasmas. Este proceso permitió corroborar la efectividad del medio y la morfología de las células fitoplasmáticas, bajo microscopia electrónica. 2019-06-30 2019-09-10T20:38:09Z 2019-09-10T20:38:09Z Journal Article https://hdl.handle.net/10568/103612 en Open Access Universidad de Ciencias Aplicadas Y Ambientales - UDCA Betancourth, Carlos; Pardo, Juan Manuel; Muñoz, Jaime & Alvarez, Elizabeth (2019). Isolation of phytoplasmas associated to frogskin disease in cassava. UDCA Revista: actualidad y divulgacion cientifica. 22(1): e1177 |
| spellingShingle | cassava pests of plants aetiology etiología cultivos industriales industrial crops Betancourth, Carlos Pardo, Juan Manuel Muñoz, Jaime E. Álvarez, Elizabeth Isolation of phytoplasmas associated to frogskin disease in cassava |
| title | Isolation of phytoplasmas associated to frogskin disease in cassava |
| title_full | Isolation of phytoplasmas associated to frogskin disease in cassava |
| title_fullStr | Isolation of phytoplasmas associated to frogskin disease in cassava |
| title_full_unstemmed | Isolation of phytoplasmas associated to frogskin disease in cassava |
| title_short | Isolation of phytoplasmas associated to frogskin disease in cassava |
| title_sort | isolation of phytoplasmas associated to frogskin disease in cassava |
| topic | cassava pests of plants aetiology etiología cultivos industriales industrial crops |
| url | https://hdl.handle.net/10568/103612 |
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