Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize

Background Common rust, caused by Puccinia sorghi, is an important foliar disease of maize that has been associated with up to 50% grain yield loss. Development of resistant maize germplasm is the ideal strategy to combat P. sorghi. Results Association mapping performed using a mixed linear model (...

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Autores principales: Zheng, H., Chen, J., Mu, C., Makumbi, Dan, Xu, Y., Mahuku, George S.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2018
Materias:
Acceso en línea:https://hdl.handle.net/10568/103497
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author Zheng, H.
Chen, J.
Mu, C.
Makumbi, Dan
Xu, Y.
Mahuku, George S.
author_browse Chen, J.
Mahuku, George S.
Makumbi, Dan
Mu, C.
Xu, Y.
Zheng, H.
author_facet Zheng, H.
Chen, J.
Mu, C.
Makumbi, Dan
Xu, Y.
Mahuku, George S.
author_sort Zheng, H.
collection Repository of Agricultural Research Outputs (CGSpace)
description Background Common rust, caused by Puccinia sorghi, is an important foliar disease of maize that has been associated with up to 50% grain yield loss. Development of resistant maize germplasm is the ideal strategy to combat P. sorghi. Results Association mapping performed using a mixed linear model (MLM), integrating population structure and family relatedness identified 25 QTL (P < 3.12 × 10− 5) that were associated with resistance to common rust and distributed on chromosomes 1, 3, 5, 6, 8, and 10. We identified three QTLs associated with all three disease parameters (final disease rating, mean disease rating, and area under disease progress curve) located on chromosomes 1, 3, and 8. A total of 5 QTLs for resistance to common rust were identified in the RIL population. Nine candidate genes located on chromosomes 1, 5, 6, 8, and 10 for resistance to common rust associated loci were identified through detailed annotation. Conclusions Using a diverse set of inbred lines genotyped with high density markers and evaluated for common rust resistance in multiple environments, it was possible to identify QTL significantly associated with resistance to common rust and several candidate genes. The results point to the need for fine mapping common rust resistance by targeting regions identified in common between this study and others using diverse germplasm.
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spelling CGSpace1034972025-11-11T10:17:08Z Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize Zheng, H. Chen, J. Mu, C. Makumbi, Dan Xu, Y. Mahuku, George S. puccinia sorghi maize diseases quantitative trait loci germplasm Background Common rust, caused by Puccinia sorghi, is an important foliar disease of maize that has been associated with up to 50% grain yield loss. Development of resistant maize germplasm is the ideal strategy to combat P. sorghi. Results Association mapping performed using a mixed linear model (MLM), integrating population structure and family relatedness identified 25 QTL (P < 3.12 × 10− 5) that were associated with resistance to common rust and distributed on chromosomes 1, 3, 5, 6, 8, and 10. We identified three QTLs associated with all three disease parameters (final disease rating, mean disease rating, and area under disease progress curve) located on chromosomes 1, 3, and 8. A total of 5 QTLs for resistance to common rust were identified in the RIL population. Nine candidate genes located on chromosomes 1, 5, 6, 8, and 10 for resistance to common rust associated loci were identified through detailed annotation. Conclusions Using a diverse set of inbred lines genotyped with high density markers and evaluated for common rust resistance in multiple environments, it was possible to identify QTL significantly associated with resistance to common rust and several candidate genes. The results point to the need for fine mapping common rust resistance by targeting regions identified in common between this study and others using diverse germplasm. 2018-12 2019-09-05T10:15:15Z 2019-09-05T10:15:15Z Journal Article https://hdl.handle.net/10568/103497 en Open Access application/pdf Springer Zheng, H., Chen, J., Mu, C., Makumbi, D., Xu, Y., & Mahuku, G. (2018). Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize. BMC Plant Biology, 18(1), 1-14.
spellingShingle puccinia sorghi
maize
diseases
quantitative trait loci
germplasm
Zheng, H.
Chen, J.
Mu, C.
Makumbi, Dan
Xu, Y.
Mahuku, George S.
Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize
title Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize
title_full Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize
title_fullStr Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize
title_full_unstemmed Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize
title_short Combined linkage and association mapping reveal QTL for host plant resistance to common rust (Puccinia sorghi) in tropical maize
title_sort combined linkage and association mapping reveal qtl for host plant resistance to common rust puccinia sorghi in tropical maize
topic puccinia sorghi
maize
diseases
quantitative trait loci
germplasm
url https://hdl.handle.net/10568/103497
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