EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences
Digitaria exilis is an important indigenous cereal in West Africa. The first fonio reference transcriptome was released and became a key tool for developing new molecular markers contributing to a better understanding of its genetic diversity. A total of 126 new putative primer pairs were successful...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/103458 |
| _version_ | 1855535358043750400 |
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| author | Olodo, K.F. Gueye, M.C. Calatayud, C. Diop, B.M. Kane, N.A. Ngom, A. Ntui, V.O. Barreto, M.M.S. Uyoh, E.A. Abraham, S. Vigouroux, Yves Billot, C. Barnaud, Adéline |
| author_browse | Abraham, S. Barnaud, Adéline Barreto, M.M.S. Billot, C. Calatayud, C. Diop, B.M. Gueye, M.C. Kane, N.A. Ngom, A. Ntui, V.O. Olodo, K.F. Uyoh, E.A. Vigouroux, Yves |
| author_facet | Olodo, K.F. Gueye, M.C. Calatayud, C. Diop, B.M. Kane, N.A. Ngom, A. Ntui, V.O. Barreto, M.M.S. Uyoh, E.A. Abraham, S. Vigouroux, Yves Billot, C. Barnaud, Adéline |
| author_sort | Olodo, K.F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Digitaria exilis is an important indigenous cereal in West Africa. The first fonio reference transcriptome was released and became a key tool for developing new molecular markers contributing to a better understanding of its genetic diversity. A total of 126 new putative primer pairs were successfully designed in 37,327 unigenes from the D. exilis transcriptome. Thirty-seven primer pairs were randomly selected and tested for their ability to cross-amplify to related species. Clear amplification patterns were observed on 24 primer pairs. Of these, 71, 74 and 35% showed polymorphism in three species: D. exilis, D. longiflora and D. iburua. The transferability from D. exilis was 96% to D. longiflora and 71% to D. iburua. The new SSR markers confirmed the close genetic proximity of D. exilis with D. longiflora and its stronger genetic difference of D. exilis from D. iburua. These markers will be valuable for completing future knowledge on Digitaria evolutionary history, and for testing gene flows between related species. |
| format | Journal Article |
| id | CGSpace103458 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace1034582025-01-27T15:00:52Z EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences Olodo, K.F. Gueye, M.C. Calatayud, C. Diop, B.M. Kane, N.A. Ngom, A. Ntui, V.O. Barreto, M.M.S. Uyoh, E.A. Abraham, S. Vigouroux, Yves Billot, C. Barnaud, Adéline digitaria microsatellites poaceae west africa Digitaria exilis is an important indigenous cereal in West Africa. The first fonio reference transcriptome was released and became a key tool for developing new molecular markers contributing to a better understanding of its genetic diversity. A total of 126 new putative primer pairs were successfully designed in 37,327 unigenes from the D. exilis transcriptome. Thirty-seven primer pairs were randomly selected and tested for their ability to cross-amplify to related species. Clear amplification patterns were observed on 24 primer pairs. Of these, 71, 74 and 35% showed polymorphism in three species: D. exilis, D. longiflora and D. iburua. The transferability from D. exilis was 96% to D. longiflora and 71% to D. iburua. The new SSR markers confirmed the close genetic proximity of D. exilis with D. longiflora and its stronger genetic difference of D. exilis from D. iburua. These markers will be valuable for completing future knowledge on Digitaria evolutionary history, and for testing gene flows between related species. 2018 2019-08-29T09:57:10Z 2019-08-29T09:57:10Z Journal Article https://hdl.handle.net/10568/103458 en Open Access Cambridge University Press Olodo, K.F., Gueye, M.C., Calatayud, C., Diop, B.M., Kane, N.A., Ngom, A., ... & Vigouroux, Y. (2018). EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences. Plant Genetic Resources, 1-5. |
| spellingShingle | digitaria microsatellites poaceae west africa Olodo, K.F. Gueye, M.C. Calatayud, C. Diop, B.M. Kane, N.A. Ngom, A. Ntui, V.O. Barreto, M.M.S. Uyoh, E.A. Abraham, S. Vigouroux, Yves Billot, C. Barnaud, Adéline EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences |
| title | EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences |
| title_full | EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences |
| title_fullStr | EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences |
| title_full_unstemmed | EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences |
| title_short | EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences |
| title_sort | est ssr development for digitaria exilis and its relatives d iburua and d longiflora from transcriptome sequences |
| topic | digitaria microsatellites poaceae west africa |
| url | https://hdl.handle.net/10568/103458 |
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