Genetic diversity in 96 accessions of cassava as revealed by Random Amplified Polymorphic DNAs (RAPDs)
Some 96 accessions of cassava (Manihot esculenta) were evaluted for genetic diversity using random amplified polymorphic DNA (RAPD) primers. Ten out of 80 Primers were considered highly informative and were used to fingerprint all of the accessions. Amplification of genomic DNA with the primers reve...
| Main Authors: | , , , , |
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| Format: | Conference Paper |
| Language: | Inglés |
| Published: |
2004
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/103340 |
| _version_ | 1855531561641836544 |
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| author | Offei, Samuel Kwame Danquah, E.Y. Okai, E. Mignouna, Hodeba D. Dixon, Alfred G.O. |
| author_browse | Danquah, E.Y. Dixon, Alfred G.O. Mignouna, Hodeba D. Offei, Samuel Kwame Okai, E. |
| author_facet | Offei, Samuel Kwame Danquah, E.Y. Okai, E. Mignouna, Hodeba D. Dixon, Alfred G.O. |
| author_sort | Offei, Samuel Kwame |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Some 96 accessions of cassava (Manihot esculenta) were evaluted for genetic diversity using random amplified polymorphic DNA (RAPD) primers. Ten out of 80 Primers were considered highly informative and were used to fingerprint all of the accessions. Amplification of genomic DNA with the primers revealed a total of 63 different banding positions ranging from 0.3 to 2.0 kb. The number of bands per primer ranged from five to nine. The RAPD patterns were highly reproducible. While no variation was observed among plants belonging to the same accession, a large number of inter-accession polymorphisms enable us to reliably discriminate between all of the accessions. |
| format | Conference Paper |
| id | CGSpace103340 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| record_format | dspace |
| spelling | CGSpace1033402024-03-06T10:16:43Z Genetic diversity in 96 accessions of cassava as revealed by Random Amplified Polymorphic DNAs (RAPDs) Offei, Samuel Kwame Danquah, E.Y. Okai, E. Mignouna, Hodeba D. Dixon, Alfred G.O. genetic diversity (as resource) cassava polymorphism manihot esculenta Some 96 accessions of cassava (Manihot esculenta) were evaluted for genetic diversity using random amplified polymorphic DNA (RAPD) primers. Ten out of 80 Primers were considered highly informative and were used to fingerprint all of the accessions. Amplification of genomic DNA with the primers revealed a total of 63 different banding positions ranging from 0.3 to 2.0 kb. The number of bands per primer ranged from five to nine. The RAPD patterns were highly reproducible. While no variation was observed among plants belonging to the same accession, a large number of inter-accession polymorphisms enable us to reliably discriminate between all of the accessions. 2004 2019-08-21T14:12:59Z 2019-08-21T14:12:59Z Conference Paper https://hdl.handle.net/10568/103340 en Limited Access Offei, S.K., Danquah, E.Y., Okai, E., Mignouna, H.D. & Dixon, A.G. (2004). Genetic diversity in 96 accessions of cassava as revealed by Random Amplified Polymorphic DNAs (RAPDs). In The small processor and development of local food industries for market economy: 8th Symposium of the International Society for Tropical Root Crops-Africa Branch (ISTRC-AB), 12-16 November 2001, IITA, Ibadan, Nigeria. Ibadan, Nigeria: IITA, (p. 476-481). |
| spellingShingle | genetic diversity (as resource) cassava polymorphism manihot esculenta Offei, Samuel Kwame Danquah, E.Y. Okai, E. Mignouna, Hodeba D. Dixon, Alfred G.O. Genetic diversity in 96 accessions of cassava as revealed by Random Amplified Polymorphic DNAs (RAPDs) |
| title | Genetic diversity in 96 accessions of cassava as revealed by Random Amplified Polymorphic DNAs (RAPDs) |
| title_full | Genetic diversity in 96 accessions of cassava as revealed by Random Amplified Polymorphic DNAs (RAPDs) |
| title_fullStr | Genetic diversity in 96 accessions of cassava as revealed by Random Amplified Polymorphic DNAs (RAPDs) |
| title_full_unstemmed | Genetic diversity in 96 accessions of cassava as revealed by Random Amplified Polymorphic DNAs (RAPDs) |
| title_short | Genetic diversity in 96 accessions of cassava as revealed by Random Amplified Polymorphic DNAs (RAPDs) |
| title_sort | genetic diversity in 96 accessions of cassava as revealed by random amplified polymorphic dnas rapds |
| topic | genetic diversity (as resource) cassava polymorphism manihot esculenta |
| url | https://hdl.handle.net/10568/103340 |
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