Tissue culture of root and tuber crops at IITA

At IITA, tissue culture methods, such as meristem, shoot-tip, and node-cutting cultures, are used routinely to eliminate virus infection from improved clones of cassava and yam and for micropropagating cassava, yam, sweet potato, and cocoyams. Virus-tested improved clones of cassava, yam, and sweet...

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Main Author: Ng, S.Y.C.
Format: Book Chapter
Language:Inglés
Published: 1992
Subjects:
Online Access:https://hdl.handle.net/10568/103290
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author Ng, S.Y.C.
author_browse Ng, S.Y.C.
author_facet Ng, S.Y.C.
author_sort Ng, S.Y.C.
collection Repository of Agricultural Research Outputs (CGSpace)
description At IITA, tissue culture methods, such as meristem, shoot-tip, and node-cutting cultures, are used routinely to eliminate virus infection from improved clones of cassava and yam and for micropropagating cassava, yam, sweet potato, and cocoyams. Virus-tested improved clones of cassava, yam, and sweet potato have been distributed to national programmes for evaluation and testing. The clonal germplasm is conserved by the in vitro reduced growth storage method; plantlets can be kept in the same tube for 1-2 years. An embryo culture technique for germinating mature embryos of Manihot species and immature embryos of cassava is being developed. Somatic embryos have been obtained in vitro from young cassava leaf material cultured in a medium containing picloram. Anther and unpollinated ovary culture of cassava, aimed at producing haploid plants, is under investigation. In vitro microtuber production of yams is an alternative that could aid international distribution of virus-tested clonal materials. The dormancy and uniform germination of microtubers need to be investigated.
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spelling CGSpace1032902023-06-08T15:13:53Z Tissue culture of root and tuber crops at IITA Ng, S.Y.C. tissue culture meristem culture cassava yams sweet potatoes At IITA, tissue culture methods, such as meristem, shoot-tip, and node-cutting cultures, are used routinely to eliminate virus infection from improved clones of cassava and yam and for micropropagating cassava, yam, sweet potato, and cocoyams. Virus-tested improved clones of cassava, yam, and sweet potato have been distributed to national programmes for evaluation and testing. The clonal germplasm is conserved by the in vitro reduced growth storage method; plantlets can be kept in the same tube for 1-2 years. An embryo culture technique for germinating mature embryos of Manihot species and immature embryos of cassava is being developed. Somatic embryos have been obtained in vitro from young cassava leaf material cultured in a medium containing picloram. Anther and unpollinated ovary culture of cassava, aimed at producing haploid plants, is under investigation. In vitro microtuber production of yams is an alternative that could aid international distribution of virus-tested clonal materials. The dormancy and uniform germination of microtubers need to be investigated. 1992 2019-08-21T14:12:52Z 2019-08-21T14:12:52Z Book Chapter https://hdl.handle.net/10568/103290 en Open Access Ng, S.Y.C. (1992). Tissue culture of root and tuber crops at IITA. In G. Thottappilly, L.M. Monti, D.R. Mohan Raj and A.W. Moore(Eds.), Biotechnology: enhancing research on tropical crops of Africa. Ibadan, Nigeria: IITA, (p.135-141).
spellingShingle tissue culture
meristem culture
cassava
yams
sweet potatoes
Ng, S.Y.C.
Tissue culture of root and tuber crops at IITA
title Tissue culture of root and tuber crops at IITA
title_full Tissue culture of root and tuber crops at IITA
title_fullStr Tissue culture of root and tuber crops at IITA
title_full_unstemmed Tissue culture of root and tuber crops at IITA
title_short Tissue culture of root and tuber crops at IITA
title_sort tissue culture of root and tuber crops at iita
topic tissue culture
meristem culture
cassava
yams
sweet potatoes
url https://hdl.handle.net/10568/103290
work_keys_str_mv AT ngsyc tissuecultureofrootandtubercropsatiita