A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. Mycoides

Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides, is an important livestock disease in Africa. The current control measures rely on a vaccine with limited efficacy and occasional severe side effects. Knowledge of the protective arms of immunity involved in this...

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Autores principales: Sacchini, F., Naessens, Jan, Awino, Elias, Heller, M., Hlinak, A., Haider, W., Sterner-Kock, Anja, Jores, Joerg
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2011
Acceso en línea:https://hdl.handle.net/10568/10259
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author Sacchini, F.
Naessens, Jan
Awino, Elias
Heller, M.
Hlinak, A.
Haider, W.
Sterner-Kock, Anja
Jores, Joerg
author_browse Awino, Elias
Haider, W.
Heller, M.
Hlinak, A.
Jores, Joerg
Naessens, Jan
Sacchini, F.
Sterner-Kock, Anja
author_facet Sacchini, F.
Naessens, Jan
Awino, Elias
Heller, M.
Hlinak, A.
Haider, W.
Sterner-Kock, Anja
Jores, Joerg
author_sort Sacchini, F.
collection Repository of Agricultural Research Outputs (CGSpace)
description Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides, is an important livestock disease in Africa. The current control measures rely on a vaccine with limited efficacy and occasional severe side effects. Knowledge of the protective arms of immunity involved in this disease will be beneficial for the development of an improved vaccine. In previous studies on cattle infected with M. mycoides subsp. mycoides, a correlation was detected between the levels of mycoplasma-specific IFN-γ-secreting CD4+ T lymphocytes and reduced clinical signs. However, no cause and effect has been established, and the role of such cells and of protective responses acquired during a primary infection is not known. We investigated the role of CD4+ T lymphocytes in CBPP by comparing disease patterns and post mortem findings between CD4+ T cell depleted and non-depleted cattle. The depletion was carried out using several injections of BoCD4 specific murine monoclonal antibody on day 6 after experimental endotracheal infection with the strain Afadé. All cattle were monitored clinically daily and sacrificed 28-30 days post-infection. Statistically significant but small differences were observed in the mortality rate between the depleted and non-depleted animals. However, no differences in clinical parameters (fever, signs of respiratory distress) and pathological lesions were observed, despite elimination of CD4+ T cells for more than a week. The slightly higher mortality in the depleted group suggests a minor role of CD4+ T cells in control of CBPP.
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spelling CGSpace102592023-12-08T19:36:04Z A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. Mycoides Sacchini, F. Naessens, Jan Awino, Elias Heller, M. Hlinak, A. Haider, W. Sterner-Kock, Anja Jores, Joerg Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides, is an important livestock disease in Africa. The current control measures rely on a vaccine with limited efficacy and occasional severe side effects. Knowledge of the protective arms of immunity involved in this disease will be beneficial for the development of an improved vaccine. In previous studies on cattle infected with M. mycoides subsp. mycoides, a correlation was detected between the levels of mycoplasma-specific IFN-γ-secreting CD4+ T lymphocytes and reduced clinical signs. However, no cause and effect has been established, and the role of such cells and of protective responses acquired during a primary infection is not known. We investigated the role of CD4+ T lymphocytes in CBPP by comparing disease patterns and post mortem findings between CD4+ T cell depleted and non-depleted cattle. The depletion was carried out using several injections of BoCD4 specific murine monoclonal antibody on day 6 after experimental endotracheal infection with the strain Afadé. All cattle were monitored clinically daily and sacrificed 28-30 days post-infection. Statistically significant but small differences were observed in the mortality rate between the depleted and non-depleted animals. However, no differences in clinical parameters (fever, signs of respiratory distress) and pathological lesions were observed, despite elimination of CD4+ T cells for more than a week. The slightly higher mortality in the depleted group suggests a minor role of CD4+ T cells in control of CBPP. 2011-12 2011-10-09T15:01:43Z 2011-10-09T15:01:43Z Journal Article https://hdl.handle.net/10568/10259 en Open Access Springer Sacchini, F., Naessens, J., Awino, E., Heller, M., Hlinak, A., Haider, W., Sterner-Kock, A. and Jores, J. 2011. A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. Mycoides. Veterinary Research 42(77)
spellingShingle Sacchini, F.
Naessens, Jan
Awino, Elias
Heller, M.
Hlinak, A.
Haider, W.
Sterner-Kock, Anja
Jores, Joerg
A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. Mycoides
title A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. Mycoides
title_full A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. Mycoides
title_fullStr A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. Mycoides
title_full_unstemmed A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. Mycoides
title_short A minor role of CD4+ T lymphocytes in the control of a primary infection of cattle with Mycoplasma mycoides subsp. Mycoides
title_sort minor role of cd4 t lymphocytes in the control of a primary infection of cattle with mycoplasma mycoides subsp mycoides
url https://hdl.handle.net/10568/10259
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