Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings
Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures ar...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Bio-Protocol, LLC
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/102052 |
| _version_ | 1855533013325053952 |
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| author | Pinel Galzi, A. Hébrard, E. Traoré, O. Silué, D. Albar, L. |
| author_browse | Albar, L. Hébrard, E. Pinel Galzi, A. Silué, D. Traoré, O. |
| author_facet | Pinel Galzi, A. Hébrard, E. Traoré, O. Silué, D. Albar, L. |
| author_sort | Pinel Galzi, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures are required for the identification and characterization of resistance sources. This paper describes a protocol for mechanical inoculation of rice seedlings with RYMV and two methods of resistance evaluation – one based on a symptom severity index and the other on virus detection through double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). |
| format | Journal Article |
| id | CGSpace102052 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Bio-Protocol, LLC |
| publisherStr | Bio-Protocol, LLC |
| record_format | dspace |
| spelling | CGSpace1020522025-01-27T15:00:52Z Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings Pinel Galzi, A. Hébrard, E. Traoré, O. Silué, D. Albar, L. research rice Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures are required for the identification and characterization of resistance sources. This paper describes a protocol for mechanical inoculation of rice seedlings with RYMV and two methods of resistance evaluation – one based on a symptom severity index and the other on virus detection through double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). 2018 2019-07-02T14:57:02Z 2019-07-02T14:57:02Z Journal Article https://hdl.handle.net/10568/102052 en Limited Access Bio-Protocol, LLC Pinel-Galzi, A., Hébrard, E., Traoré, O., Silué, D., and Albar, L. 2018. Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings. Bio-protocol 8(11):e2863 |
| spellingShingle | research rice Pinel Galzi, A. Hébrard, E. Traoré, O. Silué, D. Albar, L. Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings |
| title | Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings |
| title_full | Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings |
| title_fullStr | Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings |
| title_full_unstemmed | Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings |
| title_short | Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings |
| title_sort | protocol for rymv inoculation and resistance evaluation in rice seedlings |
| topic | research rice |
| url | https://hdl.handle.net/10568/102052 |
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