Reproductive biology in cassava: stigma receptivity and pollen tube growth

Knowledge on the reproductive biology of cassava, relevant to breeders and molecular geneticists, is still limited. Therefore, different studies were carried out to determine the duration of stigma receptivity and the rate of pollen tube growth. Inflorescences were covered for up to 3 days after the...

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Autores principales: Ramos Abril, Lisette Natalia, Pineda, Marcela, Wasek, I., Wedzony, Maria, Ceballos, Hernán
Formato: Journal Article
Lenguaje:Inglés
Publicado: Informa UK Limited 2019
Materias:
Acceso en línea:https://hdl.handle.net/10568/101956
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author Ramos Abril, Lisette Natalia
Pineda, Marcela
Wasek, I.
Wedzony, Maria
Ceballos, Hernán
author_browse Ceballos, Hernán
Pineda, Marcela
Ramos Abril, Lisette Natalia
Wasek, I.
Wedzony, Maria
author_facet Ramos Abril, Lisette Natalia
Pineda, Marcela
Wasek, I.
Wedzony, Maria
Ceballos, Hernán
author_sort Ramos Abril, Lisette Natalia
collection Repository of Agricultural Research Outputs (CGSpace)
description Knowledge on the reproductive biology of cassava, relevant to breeders and molecular geneticists, is still limited. Therefore, different studies were carried out to determine the duration of stigma receptivity and the rate of pollen tube growth. Inflorescences were covered for up to 3 days after the first opening of the bracts (e.g. anthesis day) to prevent open pollination. Results indicate that fruit and seed set are drastically reduced when flowers were covered for 2 or 3 days. However, fruits and seeds were obtained even from flowers that had been covered for 3 days after anthesis, although at low frequency. The rate of pollen tube growth was assessed in many combinations of female and male progenitors crossed through controlled pollinations and collecting the pistils at varying hours after pollination (HAP). Pollen tube growth is fast during the first 6 HAP reaching the tip of the nucellar beak. The growth slows down thereafter, taking 10 additional hours to reach the end of the beak. The growth of pollen tubes slows down even further until they enter the embryo sac. Only 10% of samples showed pollen tubes entering the embryo sac between 48 and 66 HAP. Although several tubes may reach the nucellar beak, only one was observed entering the embryo sac. Results, across the different experiments, were highly variable suggesting that the timeline of fertilization is influenced both by genotypic and environmental factors as well as the manual manipulation of inflorescences and cyathia.
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language Inglés
publishDate 2019
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spelling CGSpace1019562025-03-13T09:43:57Z Reproductive biology in cassava: stigma receptivity and pollen tube growth Ramos Abril, Lisette Natalia Pineda, Marcela Wasek, I. Wedzony, Maria Ceballos, Hernán manihot esculenta cassava Knowledge on the reproductive biology of cassava, relevant to breeders and molecular geneticists, is still limited. Therefore, different studies were carried out to determine the duration of stigma receptivity and the rate of pollen tube growth. Inflorescences were covered for up to 3 days after the first opening of the bracts (e.g. anthesis day) to prevent open pollination. Results indicate that fruit and seed set are drastically reduced when flowers were covered for 2 or 3 days. However, fruits and seeds were obtained even from flowers that had been covered for 3 days after anthesis, although at low frequency. The rate of pollen tube growth was assessed in many combinations of female and male progenitors crossed through controlled pollinations and collecting the pistils at varying hours after pollination (HAP). Pollen tube growth is fast during the first 6 HAP reaching the tip of the nucellar beak. The growth slows down thereafter, taking 10 additional hours to reach the end of the beak. The growth of pollen tubes slows down even further until they enter the embryo sac. Only 10% of samples showed pollen tubes entering the embryo sac between 48 and 66 HAP. Although several tubes may reach the nucellar beak, only one was observed entering the embryo sac. Results, across the different experiments, were highly variable suggesting that the timeline of fertilization is influenced both by genotypic and environmental factors as well as the manual manipulation of inflorescences and cyathia. 2019-01-01 2019-06-28T20:17:01Z 2019-06-28T20:17:01Z Journal Article https://hdl.handle.net/10568/101956 en Open Access Informa UK Limited Ramos Abril, Lizzeth N.; Pineda, Lizzeth M.; Wasek, I.; Wedzony, M. & Ceballos, Hernan (2019). Reproductive biology in cassava: stigma receptivity and pollen tube growth. Communicative & Integrative Biology, 12(1): 96-111
spellingShingle manihot esculenta
cassava
Ramos Abril, Lisette Natalia
Pineda, Marcela
Wasek, I.
Wedzony, Maria
Ceballos, Hernán
Reproductive biology in cassava: stigma receptivity and pollen tube growth
title Reproductive biology in cassava: stigma receptivity and pollen tube growth
title_full Reproductive biology in cassava: stigma receptivity and pollen tube growth
title_fullStr Reproductive biology in cassava: stigma receptivity and pollen tube growth
title_full_unstemmed Reproductive biology in cassava: stigma receptivity and pollen tube growth
title_short Reproductive biology in cassava: stigma receptivity and pollen tube growth
title_sort reproductive biology in cassava stigma receptivity and pollen tube growth
topic manihot esculenta
cassava
url https://hdl.handle.net/10568/101956
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AT pinedamarcela reproductivebiologyincassavastigmareceptivityandpollentubegrowth
AT waseki reproductivebiologyincassavastigmareceptivityandpollentubegrowth
AT wedzonymaria reproductivebiologyincassavastigmareceptivityandpollentubegrowth
AT ceballoshernan reproductivebiologyincassavastigmareceptivityandpollentubegrowth