Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii
Shoot-tips 15cm long from 15 soybean cultivars and breeding lines were individually immersed in Hoagland's solution in 1 × 14 cm test tubes, and supported by cotton plugs. All leaves were removed leaving about 1 cm of each petiole on the shoot. A 4 mm mycelial plug of Sclerotium rolfsii Sacc., taken...
| Main Authors: | , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
1991
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/101772 |
| _version_ | 1855539157954199552 |
|---|---|
| author | Akem, C. Dashiell, Kenton E. |
| author_browse | Akem, C. Dashiell, Kenton E. |
| author_facet | Akem, C. Dashiell, Kenton E. |
| author_sort | Akem, C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Shoot-tips 15cm long from 15 soybean cultivars and breeding lines were individually immersed in Hoagland's solution in 1 × 14 cm test tubes, and supported by cotton plugs. All leaves were removed leaving about 1 cm of each petiole on the shoot. A 4 mm mycelial plug of Sclerotium rolfsii Sacc., taken from the periphery of a 3-day-old culture grown on acidified potato dextrose agar (PDA) media was placed between the stem and a petiole in the middle of the shoot. Tubes with shoots were then placed in a polyethylene enclosure in a growth room where the day and night temperatures averaged 31 ± 2°C and 24 ± 2°C, respectively. Relative humidity (r.h.) was maintained at 80–90% by lining the bottom of the enclosure with wet burlap. Lesions appeared on shoot tips 2 days after inoculation, and their length was measured 2, 3 and 4 days later. On three cultivars, TG × 1436-1 E, TG × 1596-2E and TG × 1614-1E, the rate of lesion expansion was significantly less than that on the other cultivars. One week after inoculation, tubes were drained, and shoots left in the chamber at 50–60% r.h. to allow sclerotia to form. Sclerotia from each shoot were collected. The cultivars TG × 1436-1 D and TG × 1596-2E produced the fewest sclerotia per shoot-tip. Sclerotial viability, determined by germination on PDA at 25 ± 2°C in darkness, ranged from 38 to 99%. This method is effective in differentiating reactions of soybean cultivars to S. rolfsii. |
| format | Journal Article |
| id | CGSpace101772 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1991 |
| publishDateRange | 1991 |
| publishDateSort | 1991 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1017722024-08-27T10:37:08Z Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii Akem, C. Dashiell, Kenton E. soybeans breeding leaves petioles potatoes inoculation shoots Shoot-tips 15cm long from 15 soybean cultivars and breeding lines were individually immersed in Hoagland's solution in 1 × 14 cm test tubes, and supported by cotton plugs. All leaves were removed leaving about 1 cm of each petiole on the shoot. A 4 mm mycelial plug of Sclerotium rolfsii Sacc., taken from the periphery of a 3-day-old culture grown on acidified potato dextrose agar (PDA) media was placed between the stem and a petiole in the middle of the shoot. Tubes with shoots were then placed in a polyethylene enclosure in a growth room where the day and night temperatures averaged 31 ± 2°C and 24 ± 2°C, respectively. Relative humidity (r.h.) was maintained at 80–90% by lining the bottom of the enclosure with wet burlap. Lesions appeared on shoot tips 2 days after inoculation, and their length was measured 2, 3 and 4 days later. On three cultivars, TG × 1436-1 E, TG × 1596-2E and TG × 1614-1E, the rate of lesion expansion was significantly less than that on the other cultivars. One week after inoculation, tubes were drained, and shoots left in the chamber at 50–60% r.h. to allow sclerotia to form. Sclerotia from each shoot were collected. The cultivars TG × 1436-1 D and TG × 1596-2E produced the fewest sclerotia per shoot-tip. Sclerotial viability, determined by germination on PDA at 25 ± 2°C in darkness, ranged from 38 to 99%. This method is effective in differentiating reactions of soybean cultivars to S. rolfsii. 1991-08 2019-06-25T17:09:07Z 2019-06-25T17:09:07Z Journal Article https://hdl.handle.net/10568/101772 en Limited Access Elsevier |
| spellingShingle | soybeans breeding leaves petioles potatoes inoculation shoots Akem, C. Dashiell, Kenton E. Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii |
| title | Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii |
| title_full | Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii |
| title_fullStr | Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii |
| title_full_unstemmed | Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii |
| title_short | Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii |
| title_sort | detached shoot technique to evaluate the reaction of soybean cultivars to sclerotium rolfsii |
| topic | soybeans breeding leaves petioles potatoes inoculation shoots |
| url | https://hdl.handle.net/10568/101772 |
| work_keys_str_mv | AT akemc detachedshoottechniquetoevaluatethereactionofsoybeancultivarstosclerotiumrolfsii AT dashiellkentone detachedshoottechniquetoevaluatethereactionofsoybeancultivarstosclerotiumrolfsii |