In vitro conservation and distribution of root and tuber crop germplasm
The in vitro methods that can be used or are in use in root crop germplasm conservation and distribution are described, namely (1) normal growth storage, (2) reduced growth storage (reduced incubation temp., manipulation of culture media, both of the former), and (3) cryopreservation. The achievemen...
| Main Author: | |
|---|---|
| Format: | Book Chapter |
| Language: | Inglés |
| Published: |
1991
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/101715 |
| _version_ | 1855534960993107968 |
|---|---|
| author | Ng, S.Y.C. |
| author_browse | Ng, S.Y.C. |
| author_facet | Ng, S.Y.C. |
| author_sort | Ng, S.Y.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The in vitro methods that can be used or are in use in root crop germplasm conservation and distribution are described, namely (1) normal growth storage, (2) reduced growth storage (reduced incubation temp., manipulation of culture media, both of the former), and (3) cryopreservation. The achievements of applying some of these methods are summarized and the advantages of using tissue culture materials in germplasm exchange are highlighted. In the case of reduced incubation temp. for cassava, node explants are stored in plantlet culture type at either 20 degrees C for 18-24 mo. or in liquid media with filter paper bridge incubated at 20 degrees C and with a 16-h photoperiod with 100-200 lux light intensity. When culture media were manipulated, cassava node explants are used in plantlet culture type and stored for 2 yr in large culture vessels, with culture media containing low osmotic concn. and activated charcoal |
| format | Book Chapter |
| id | CGSpace101715 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1991 |
| publishDateRange | 1991 |
| publishDateSort | 1991 |
| record_format | dspace |
| spelling | CGSpace1017152023-06-08T20:14:13Z In vitro conservation and distribution of root and tuber crop germplasm Ng, S.Y.C. root crops tissue culture germplasm cassava The in vitro methods that can be used or are in use in root crop germplasm conservation and distribution are described, namely (1) normal growth storage, (2) reduced growth storage (reduced incubation temp., manipulation of culture media, both of the former), and (3) cryopreservation. The achievements of applying some of these methods are summarized and the advantages of using tissue culture materials in germplasm exchange are highlighted. In the case of reduced incubation temp. for cassava, node explants are stored in plantlet culture type at either 20 degrees C for 18-24 mo. or in liquid media with filter paper bridge incubated at 20 degrees C and with a 16-h photoperiod with 100-200 lux light intensity. When culture media were manipulated, cassava node explants are used in plantlet culture type and stored for 2 yr in large culture vessels, with culture media containing low osmotic concn. and activated charcoal 1991 2019-06-25T17:09:01Z 2019-06-25T17:09:01Z Book Chapter https://hdl.handle.net/10568/101715 en Limited Access Ng, S.Y.C. (1991). In vitro conservation and distribution of root and tuber crop germplasm. In: A. Frank, H. Zedan, N.Q. Ng & P. Perrino, International Workshop on Crop Genetic Resources of Africa. Nairobi, Kenya: Crop Genetic Resources of Africa: IBPGR, (p.95-106). |
| spellingShingle | root crops tissue culture germplasm cassava Ng, S.Y.C. In vitro conservation and distribution of root and tuber crop germplasm |
| title | In vitro conservation and distribution of root and tuber crop germplasm |
| title_full | In vitro conservation and distribution of root and tuber crop germplasm |
| title_fullStr | In vitro conservation and distribution of root and tuber crop germplasm |
| title_full_unstemmed | In vitro conservation and distribution of root and tuber crop germplasm |
| title_short | In vitro conservation and distribution of root and tuber crop germplasm |
| title_sort | in vitro conservation and distribution of root and tuber crop germplasm |
| topic | root crops tissue culture germplasm cassava |
| url | https://hdl.handle.net/10568/101715 |
| work_keys_str_mv | AT ngsyc invitroconservationanddistributionofrootandtubercropgermplasm |