Genetic transformation of banana and plantain (Musa spp.) via particle bombardment
We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
1995
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/101091 |
| _version_ | 1855522079341805568 |
|---|---|
| author | Sági, L. Panis, Bartholomeus Remy, S. Schoofs, H. Smet, K. de Swennen, Rony L. Cammue, B.P.A. |
| author_browse | Cammue, B.P.A. Panis, Bartholomeus Remy, S. Schoofs, H. Smet, K. de Swennen, Rony L. Sági, L. |
| author_facet | Sági, L. Panis, Bartholomeus Remy, S. Schoofs, H. Smet, K. de Swennen, Rony L. Cammue, B.P.A. |
| author_sort | Sági, L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient expression of the β-glucuronidase gene in both banana and plantain cells. Bombarded banana cells were selected with hygromycin and regenerated into plants. Molecular and histochemical characterization of transformants revealed the stable integration of the transferred genes into the banana genome. |
| format | Journal Article |
| id | CGSpace101091 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| record_format | dspace |
| spelling | CGSpace1010912023-09-25T09:16:55Z Genetic transformation of banana and plantain (Musa spp.) via particle bombardment Sági, L. Panis, Bartholomeus Remy, S. Schoofs, H. Smet, K. de Swennen, Rony L. Cammue, B.P.A. bananas plantains genes We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient expression of the β-glucuronidase gene in both banana and plantain cells. Bombarded banana cells were selected with hygromycin and regenerated into plants. Molecular and histochemical characterization of transformants revealed the stable integration of the transferred genes into the banana genome. 1995 2019-04-24T12:29:52Z 2019-04-24T12:29:52Z Journal Article https://hdl.handle.net/10568/101091 en Limited Access Sagi, L., Panis, B., Remy, S., Schoofs, H., De Smet, K., Swennen, R. & Cammue, B.P. (1995). Genetic transformation of banana and plantain (Musa spp.) via particle bombardment. Nature Biotechnology, 13, 481-485. |
| spellingShingle | bananas plantains genes Sági, L. Panis, Bartholomeus Remy, S. Schoofs, H. Smet, K. de Swennen, Rony L. Cammue, B.P.A. Genetic transformation of banana and plantain (Musa spp.) via particle bombardment |
| title | Genetic transformation of banana and plantain (Musa spp.) via particle bombardment |
| title_full | Genetic transformation of banana and plantain (Musa spp.) via particle bombardment |
| title_fullStr | Genetic transformation of banana and plantain (Musa spp.) via particle bombardment |
| title_full_unstemmed | Genetic transformation of banana and plantain (Musa spp.) via particle bombardment |
| title_short | Genetic transformation of banana and plantain (Musa spp.) via particle bombardment |
| title_sort | genetic transformation of banana and plantain musa spp via particle bombardment |
| topic | bananas plantains genes |
| url | https://hdl.handle.net/10568/101091 |
| work_keys_str_mv | AT sagil genetictransformationofbananaandplantainmusasppviaparticlebombardment AT panisbartholomeus genetictransformationofbananaandplantainmusasppviaparticlebombardment AT remys genetictransformationofbananaandplantainmusasppviaparticlebombardment AT schoofsh genetictransformationofbananaandplantainmusasppviaparticlebombardment AT smetkde genetictransformationofbananaandplantainmusasppviaparticlebombardment AT swennenronyl genetictransformationofbananaandplantainmusasppviaparticlebombardment AT cammuebpa genetictransformationofbananaandplantainmusasppviaparticlebombardment |