RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata [L.] Walp
Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
1996
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/101026 |
| _version_ | 1855530599843889152 |
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| author | Myers, G.O. Fatokun, C.A. Young, N.D. |
| author_browse | Fatokun, C.A. Myers, G.O. Young, N.D. |
| author_facet | Myers, G.O. Fatokun, C.A. Young, N.D. |
| author_sort | Myers, G.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation of associations between genes of interest and RFLP markers. A cross between an aphid (Aphis craccivora Koch) resistant cultivated cowpea, IT84S-2246-4, and an aphid susceptible wild cowpea, N1 963, was screened for both aphid phenotype and RFLP marker segregation. One RFLP marker, bg4D9b, was found to be tightly linked to the aphid resistance gene (Rac!) and several flanking markers in the same linkage group (linkage group 1) were also identified. The close association of Rac! and RFLP bg4D9b presents a real potential for cloning this insect resistance gene. |
| format | Journal Article |
| id | CGSpace101026 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1996 |
| publishDateRange | 1996 |
| publishDateSort | 1996 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1010262024-05-15T05:11:50Z RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata [L.] Walp Myers, G.O. Fatokun, C.A. Young, N.D. aphis craccivora cowpeas insects legumes vigna unguiculata Restriction fragment length polymorphism (RFLP) analysis has several advantages over traditional methods of genetic linkage mapping, one of these being the starting point for map-based cloning. The recent development of an RFLP map of cowpea (Vigna unguiculata L. Walp) has allowed the investigation of associations between genes of interest and RFLP markers. A cross between an aphid (Aphis craccivora Koch) resistant cultivated cowpea, IT84S-2246-4, and an aphid susceptible wild cowpea, N1 963, was screened for both aphid phenotype and RFLP marker segregation. One RFLP marker, bg4D9b, was found to be tightly linked to the aphid resistance gene (Rac!) and several flanking markers in the same linkage group (linkage group 1) were also identified. The close association of Rac! and RFLP bg4D9b presents a real potential for cloning this insect resistance gene. 1996-01 2019-04-24T12:29:46Z 2019-04-24T12:29:46Z Journal Article https://hdl.handle.net/10568/101026 en Limited Access Springer Myers, G.O., Fatokun, C.A. & Young, N.D. (1996). RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata L. Walp). Euphatica, 91(2), 181-187. |
| spellingShingle | aphis craccivora cowpeas insects legumes vigna unguiculata Myers, G.O. Fatokun, C.A. Young, N.D. RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata [L.] Walp |
| title | RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata [L.] Walp |
| title_full | RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata [L.] Walp |
| title_fullStr | RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata [L.] Walp |
| title_full_unstemmed | RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata [L.] Walp |
| title_short | RFLP mapping of an aphid resistance gene in cowpea (Vigna unguiculata [L.] Walp |
| title_sort | rflp mapping of an aphid resistance gene in cowpea vigna unguiculata l walp |
| topic | aphis craccivora cowpeas insects legumes vigna unguiculata |
| url | https://hdl.handle.net/10568/101026 |
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