Production and distribution of virusfree yam (Dioscorea rotundata Poir.)

The international distribution of vegetative plant parts is restricted by the risks of introducing diseases and pests to non-infected areas. Methods used at IITA to produce and distribute virus-free yams to requesting national programs and collaborators are described. The selected yam clones are cle...

Full description

Bibliographic Details
Main Author: Ng, S.Y.C.
Format: Conference Paper
Language:Inglés
Published: International Society for Horticultural Science 1994
Subjects:
Online Access:https://hdl.handle.net/10568/101016
_version_ 1855521210326056960
author Ng, S.Y.C.
author_browse Ng, S.Y.C.
author_facet Ng, S.Y.C.
author_sort Ng, S.Y.C.
collection Repository of Agricultural Research Outputs (CGSpace)
description The international distribution of vegetative plant parts is restricted by the risks of introducing diseases and pests to non-infected areas. Methods used at IITA to produce and distribute virus-free yams to requesting national programs and collaborators are described. The selected yam clones are cleaned from virus disease infection through thermotherapy and meristem culture. The regenerated plants are virus indexed. The negatively indexed plants are then multiplied in vitro for international distribution. Plantlets of about 4–6 cm height grown in sterile culture media are packed for distribution by hand carriage. Node cuttings of the in vitro plantlets are also sub-cultured in a medium containing sucrose (5%) for micro-tuber production. Micro-tubers are harvested from 4–5 months old cultures and packed in sterile petri plates with sterile moist cotton wool for dispatch to national programs. Some virus-free yam plantlets are also transplanted to sterile soil in a screenhouse where they grow to maturity. Harvested tubers are used for distribution. The advantages and disadvantages of each method are discussed.
format Conference Paper
id CGSpace101016
institution CGIAR Consortium
language Inglés
publishDate 1994
publishDateRange 1994
publishDateSort 1994
publisher International Society for Horticultural Science
publisherStr International Society for Horticultural Science
record_format dspace
spelling CGSpace1010162024-05-15T05:11:29Z Production and distribution of virusfree yam (Dioscorea rotundata Poir.) Ng, S.Y.C. pests viruses yams harvesting The international distribution of vegetative plant parts is restricted by the risks of introducing diseases and pests to non-infected areas. Methods used at IITA to produce and distribute virus-free yams to requesting national programs and collaborators are described. The selected yam clones are cleaned from virus disease infection through thermotherapy and meristem culture. The regenerated plants are virus indexed. The negatively indexed plants are then multiplied in vitro for international distribution. Plantlets of about 4–6 cm height grown in sterile culture media are packed for distribution by hand carriage. Node cuttings of the in vitro plantlets are also sub-cultured in a medium containing sucrose (5%) for micro-tuber production. Micro-tubers are harvested from 4–5 months old cultures and packed in sterile petri plates with sterile moist cotton wool for dispatch to national programs. Some virus-free yam plantlets are also transplanted to sterile soil in a screenhouse where they grow to maturity. Harvested tubers are used for distribution. The advantages and disadvantages of each method are discussed. 1994-11 2019-04-24T12:29:44Z 2019-04-24T12:29:44Z Conference Paper https://hdl.handle.net/10568/101016 en Open Access International Society for Horticultural Science Ng, S.Y.C. (1994). Production and distribution of virus-free yam (Dioscorea rotundata Poir.). In F. Ofori and S.K. Hahn (Eds.), Tropical root crops in a developing economy: Proceedings of the 9th Symposium of the International Society for Tropical Root Crops, 20-26 October 1991, Accra, Ghana: IITA, (p. 324-328).
spellingShingle pests
viruses
yams
harvesting
Ng, S.Y.C.
Production and distribution of virusfree yam (Dioscorea rotundata Poir.)
title Production and distribution of virusfree yam (Dioscorea rotundata Poir.)
title_full Production and distribution of virusfree yam (Dioscorea rotundata Poir.)
title_fullStr Production and distribution of virusfree yam (Dioscorea rotundata Poir.)
title_full_unstemmed Production and distribution of virusfree yam (Dioscorea rotundata Poir.)
title_short Production and distribution of virusfree yam (Dioscorea rotundata Poir.)
title_sort production and distribution of virusfree yam dioscorea rotundata poir
topic pests
viruses
yams
harvesting
url https://hdl.handle.net/10568/101016
work_keys_str_mv AT ngsyc productionanddistributionofvirusfreeyamdioscorearotundatapoir