Post transcriptional silencing of reporter transgenes in tobacco correlates whit DNA methylation

Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correla...

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Bibliographic Details
Main Authors: Ingelbrecht, I.L., Houdt, H. van, Montagu, M. van, Depicker, A.
Format: Conference Paper
Language:Inglés
Published: National Academy of Sciences 1994
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Online Access:https://hdl.handle.net/10568/100950
Description
Summary:Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.