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The use of RAPD for identifying and classifying Musa germplasm

Using the technique of random amplified polymorphic DNA (RAPD), we have identified 116 amplification products in Musa germplasm using nine primers. This has enabled us to identify RAPD markers that are specific to each of nine genotypes of Musa representing AA, AAA, AAB, ABB, and BB genomes. The pat...

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Autores principales: Howell, E.C., Newbury, H.J., Swennen, Rony L., Withers, Lyndsey A., Ford-Lloyd, B.V.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Canadian Science Publishing 1994
Materias:
musa
genomes
bananas
plantains
genetic variation
genetics
molecular biology
biotechnology
Acceso en línea:https://hdl.handle.net/10568/100891
Sumario:Using the technique of random amplified polymorphic DNA (RAPD), we have identified 116 amplification products in Musa germplasm using nine primers. This has enabled us to identify RAPD markers that are specific to each of nine genotypes of Musa representing AA, AAA, AAB, ABB, and BB genomes. The pattern of variation observed following the application of multivariate analyses to the RAPDs banding data is very similar to the pattern of variation defined using morphological characters and used to assign Musa material into the different genome classes.

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