A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya
Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/100729 |
| _version_ | 1855516096014057472 |
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| author | Lindahl, Johanna F. Ragan, I. Rowland, R.R. Wainaina, M. Mbotha, D. Wilson, W. |
| author_browse | Lindahl, Johanna F. Mbotha, D. Ragan, I. Rowland, R.R. Wainaina, M. Wilson, W. |
| author_facet | Lindahl, Johanna F. Ragan, I. Rowland, R.R. Wainaina, M. Mbotha, D. Wilson, W. |
| author_sort | Lindahl, Johanna F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. We evaluated a multiplexing fluorescence microsphere immunoassay (FMIA) for the detection of IgG and IgM antibodies in ruminant sera against the RVFV nucleocapsid Np, glycoprotein Gn, and non-structural protein NSs. Sheep and cattle sera from a region in Kenya with previous outbreaks were tested by FMIA and two commercially available competitive ELISAs (BDSL and IDvet). Our results revealed strong detection of RVFV antibodies against the Np, Gn and NSs antigen targets. Additionally, testing of samples with FMIA Np and Gn had 100% agreement with the IDvet ELISA. The targets developed in the FMIA assay provided a basis for a larger ruminant disease panel that can simultaneously screen several abortive and zoonotic pathogens. |
| format | Journal Article |
| id | CGSpace100729 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1007292024-11-15T08:52:08Z A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya Lindahl, Johanna F. Ragan, I. Rowland, R.R. Wainaina, M. Mbotha, D. Wilson, W. zoonoses immunology diagnosis virology Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. We evaluated a multiplexing fluorescence microsphere immunoassay (FMIA) for the detection of IgG and IgM antibodies in ruminant sera against the RVFV nucleocapsid Np, glycoprotein Gn, and non-structural protein NSs. Sheep and cattle sera from a region in Kenya with previous outbreaks were tested by FMIA and two commercially available competitive ELISAs (BDSL and IDvet). Our results revealed strong detection of RVFV antibodies against the Np, Gn and NSs antigen targets. Additionally, testing of samples with FMIA Np and Gn had 100% agreement with the IDvet ELISA. The targets developed in the FMIA assay provided a basis for a larger ruminant disease panel that can simultaneously screen several abortive and zoonotic pathogens. 2019-07 2019-04-10T14:22:28Z 2019-04-10T14:22:28Z Journal Article https://hdl.handle.net/10568/100729 en Limited Access Elsevier Lindahl, J.F., Ragan, I., Rowland, R.R., Wainaina, M., Mbotha, D. and Wilson, W. 2019. A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya. Journal of Virological Methods 269: 70–76. |
| spellingShingle | zoonoses immunology diagnosis virology Lindahl, Johanna F. Ragan, I. Rowland, R.R. Wainaina, M. Mbotha, D. Wilson, W. A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya |
| title | A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya |
| title_full | A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya |
| title_fullStr | A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya |
| title_full_unstemmed | A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya |
| title_short | A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya |
| title_sort | multiplex fluorescence microsphere immunoassay for increased understanding of rift valley fever immune responses in ruminants in kenya |
| topic | zoonoses immunology diagnosis virology |
| url | https://hdl.handle.net/10568/100729 |
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