Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug
The cowpea beetle, Callosobruchus maculatus, and pod-sucking bug, Clavigralla tomentosicollis, are two of the major insect pests of cowpea in Africa. A lectin was purified from the seeds of the African yam bean (AYB), Sphenostylis stenocarpa, by affinity chromatography on Galactosc-Sepharose 4B. The...
| Autores principales: | , , |
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| Formato: | Conference Paper |
| Lenguaje: | Inglés |
| Publicado: |
2002
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/100005 |
| _version_ | 1855516510127128576 |
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| author | Okeola, O.G. Machuka, J. Fasidi, I.O. |
| author_browse | Fasidi, I.O. Machuka, J. Okeola, O.G. |
| author_facet | Okeola, O.G. Machuka, J. Fasidi, I.O. |
| author_sort | Okeola, O.G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The cowpea beetle, Callosobruchus maculatus, and pod-sucking bug, Clavigralla tomentosicollis, are two of the major insect pests of cowpea in Africa. A lectin was purified from the seeds of the African yam bean (AYB), Sphenostylis stenocarpa, by affinity chromatography on Galactosc-Sepharose 4B. The purified AYB lectin (AYBL) was tested on the two insect pests of cowpea. When C. maculatus larvae were fed on artificial cowpea seed containing 0.2, 2, and 5% (w/w) of dietary lectin, larval mortality ranged from 30 to 88% and delay in number of days to first emer gence from 4-13 days. When AYBL was tested on C. tomentosicollis, nymphal mortalities ranged from 76 to 81% at 1% and 87 to 94% at 2%. From 4 to 8%, no nymph survived up to six days after infestation. The results of these insect bioassays provided a scientific basis for isolating a lectin gene from AYB for the transformation of cowpea. |
| format | Conference Paper |
| id | CGSpace100005 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| record_format | dspace |
| spelling | CGSpace1000052023-06-08T13:54:35Z Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug Okeola, O.G. Machuka, J. Fasidi, I.O. chromatography seed insects galactose The cowpea beetle, Callosobruchus maculatus, and pod-sucking bug, Clavigralla tomentosicollis, are two of the major insect pests of cowpea in Africa. A lectin was purified from the seeds of the African yam bean (AYB), Sphenostylis stenocarpa, by affinity chromatography on Galactosc-Sepharose 4B. The purified AYB lectin (AYBL) was tested on the two insect pests of cowpea. When C. maculatus larvae were fed on artificial cowpea seed containing 0.2, 2, and 5% (w/w) of dietary lectin, larval mortality ranged from 30 to 88% and delay in number of days to first emer gence from 4-13 days. When AYBL was tested on C. tomentosicollis, nymphal mortalities ranged from 76 to 81% at 1% and 87 to 94% at 2%. From 4 to 8%, no nymph survived up to six days after infestation. The results of these insect bioassays provided a scientific basis for isolating a lectin gene from AYB for the transformation of cowpea. 2002 2019-03-03T05:54:38Z 2019-03-03T05:54:38Z Conference Paper https://hdl.handle.net/10568/100005 en Limited Access Okeola, O.G., Machuka, J. & Fasidi, I.O. (2002). Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod-sucking bug. In C.A. Fatokun, S.A. Tarawali, B.B. Singh, P.M. Kormawa and M. Tamo, Challenges and opportunities for enhancing sustainable cowpea production. Proceedings of the World Cowpea Conference III held at the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria, 4–8 September 2000. Ibadan, Nigeria: IITA. (p. 223-230). |
| spellingShingle | chromatography seed insects galactose Okeola, O.G. Machuka, J. Fasidi, I.O. Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug |
| title | Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug |
| title_full | Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug |
| title_fullStr | Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug |
| title_full_unstemmed | Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug |
| title_short | Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug |
| title_sort | insecticidal activities of the african yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug |
| topic | chromatography seed insects galactose |
| url | https://hdl.handle.net/10568/100005 |
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