Resultados de búsqueda - Time 100

Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction por Capote, Nieves, Bertolini, Edson, Martínez, M. Carmen, Olmos, Antonio, Gorris, María T., Cambra, Mariano

“…Spot real-time RT-PCR resulted 100-times more sensitive than DASI-ELISA and 10-times more sensitive than Co-PCR, although conventional real-time PCR was the most sensitive method for PPV detection. …”
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Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids por Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano

“…The number of viruliferous aphids detected by real-time RT-PCR and nested RT-PCR in a single closed tube was similar in parallel assays, nevertheless the sensitivity provided by real-time RT-PCR was 100 times higher than nested RT-PCR and 1000 times higher than DASI-ELISA and conventional RT-PCR. …”
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Tissue-print and squash real-time PCR for direct detection of 'Candidatus Liberibacter' species in citrus plants and psyllid vectors por Bertolini, Edson, Felipe, R. T. A., Sauer, A. V., Lopes, S. A., Arilla, A., Vidal, Eduardo, Mourao Filho, F. A. A., Nunes, W. M. C., Bove, J. M., López, María M., Cambra, Mariano

“…Primers were designed and used with TaqMan chemistry for accurate detection of the bacterium in immobilized targets (prints of 10 overlapping leaf pedicels per tree, or squashed single vectors), by extraction with water and direct use for real-time PCR. This simplified method was validated and could detect HLB-liberibacters in 100% of leaves with symptoms and 59% of symptomless leaves collected from HLB-infected trees. …”
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Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence por Delpiazzo, Rafael, Barcellos, Maila, Barros, Sofia, Bentacor, Laura, Fraga, Martin, Gil, Jorge, Iraola, Gregorio, Morsella, Claudia Graciela, Paolicchi, Fernando, Perez, Ruben, Riet Correa, Franklin, Sanguinetti, Margarita, Silva Silveira, Caroline da, Caballeros, Lucía

“…The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). …”
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Microstructural Mechanical Texture Evaluation of Food Materials Using Nanoindentation as a Function of Drying Time (Case Study: Baking of Cookies) por Cisneros M., Karla C.

“…In fact, microstructure cell wall strength is closely related to the micromechanical properties and texture within the range of 0.1-100 µm. Despite this, limited research has been conducted on the micromechanical properties of baked goods. …”
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Assessment of On-Farm Saved Chickpea (Cicer arietinum L.)Seed Quality in Central Ethiopia por Sori, Abebe, Bishaw, Zewdie, Fikre, Asnake, Dejene, Mashilla, Kaske, Karta

“…Two rounds of on-farm seed samples were collected at early storage in March and at planting time in September 2022. Seed quality tests, including physical purity, physiological quality, and seed health were conducted using standard laboratory techniques for the seed samples. …”
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Comparison of real-time PCR and nested PCR based on the HlyA gene for the detection of Listeria monocytogenes. Application on cheese samples por Zbrun, María Virginia, Moreno, Nadia, Camussone, Cecilia, Signorini Porchiett, Marcelo Lisandro, Primo, María Evangelina

“…At this time, the HlyA gene was amplified in all contaminated samples. …”
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Vitrification of goat, sheep, and cattle skin samples from whole ear extirpated after death and maintained at different storage times and temperatures por Silvestre, Miguel A., Sanchez, J. P., Gómez, Ernesto A.

“…In goat and sheep, no differences in attachment (100%: goat; 90-100%: sheep) or subconfluence (75-100%: goat; 70-100%: sheep) rates were observed between experimental groups. …”
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Response surface methodology study on the effects of sodium chloride and sodium tripolyphosphate concentrations, pressure level and holding time on beef patties properties por Szerman, Natalia, Ferrari, Romina, Sancho, Ana Maria, Vaudagna, Sergio Ramon

“…A Box-Behnken design was applied to evaluate the effects of sodium chloride (NaCl, 0–2%) and sodium tripolyphosphate (STPP, 0–0.5%) concentrations, pressure level (100–300 MPa) and holding time (1–5min) on technological parameters, physicochemical and texture properties of beef patties. …”
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Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection por Teresani, Gabriela, Bertolini, Edson, Alfaro-Fernández, Ana, Martínez, M. Carmen, Ossamu Tanaka, Francisco Andre, Kitajima, Elliot W., Roselló, Montserrat, Sanjuan, Susana, Carlos Ferrandiz, Juan, López, María M., Cambra, Mariano, Font-San-Ambrosio, Isabel

“…Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of ‘Ca. …”
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Simple but Effective Sand Storage Technology for Assuring More Food and Timely Planting Material in Drought-Prone Areas of SubSaharan Africa por Abidin, P.E., Carey, E.E., Kasembe, J., Akansake, D., Dorgbetor, I., Suleman, I., Achibase, S., Dery, E., Mourik, T.V., Low, Jan W.

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Effect of harvesting time on root yield and nutritional composition of orange-fleshed sweet potato [Ipomoea batatas (L.) Lam] varieties in East Hararghe por Bedassa, C.B., Gebeyehu, S., Mohammed, W., Gelmesa, D., Neme, G.

“…Harvesting Alamura at 150 DAP also had the highest ꞵ-carotene, zinc, and iron contents of 11809 μg/100 g, 3.79, and 14.47 mg/100 g, respectively. It was concluded that growing the Alamura variety and harvesting at 150 DAP was better for obtaining higher root yield with good nutritional compositions in the study area.…”
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‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia por Morán, Félix, Ben Othmen, Soukaina, Navarro-Herrero, Inmaculada, Barbé, Silvia, Martínez, M. Carmen, Marco-Noales, Ester, Chermiti, Brahim, López, María M.

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Respuesta agronomica de la yuca a duracion y modalidad del almacenamiento de estacas por Leihner, D.E., Andrade, A.S.

“…Final percentage of sprouting (95-100) was not affected by storage time when this was adequate. …”
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Transmission of ‘Candidatus Liberibacter solanacearum’ by Bactericera trigonica Hodkinson to vegetable hosts por Teresani, Gabriela, Hernández-Suárez, Estrella, Bertolini, Edson, Siverio, Felipe, Moreno, Aránzazu, Fereres, Alberto, Cambra, Mariano

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Development of a duplex TaqMan Real-Time Polymerase Chain Reaction for accurate identification and quantification of Salmonella enteritidis from laboratory samples and contaminated... por Xiong, D., Zhou, Y., Song, L., Liu, B., Matchawe, C., Chen, X., Pelle, Roger, Jiao, X., Pan, Z.

“…The duplex real-time PCR assay detected four copies of S. enteritidis DNA per reaction. …”
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Intensive plant productions - “Proalim Km 0”, in times of a Covid-19 pandemic – Part 1. Predisposition of consumers of vegetables, fruits and medicinal aromatics to their productio... por Bellaccomo, Maria Carolina, Berriolo, Maria Jimena, Caracotche, Maria Veronica, Castagnino, Ana María, Cendon, Maria Laura, Díaz, K.E., Gonzalez Ferrin, Maria Soledad, Martinoia, G.I., Mairosser, Andrea, Rogers, W.J., Villagra, Constanza Marcela, Zazzetta, Malvina Lis

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Quadruplex real-time TaqMan® RT-qPCR assay for differentiation of equine group A and B rotaviruses and Identification of group A G3 and G14 genotypes por Carossino, Mariano, Balasuriya, Udeni B.R., Thieulent, Côme J., Barrandeguy, Maria Edith, Vissani, Maria Aldana, Parreño, Gladys Viviana

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Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples por Carossino, Mariano, Barrandeguy, Maria Edith, Erol, Erdal, Li, Yanqiu, Balasuriya, Udeni B.R.

“…Current genotyping methods available for ERVA and rotaviruses affecting other animal species rely on Sanger sequencing and are significantly time-consuming, costly and labor intensive. Here, we developed the first one-step multiplex TaqMan® real-time reverse transcription polymerase chain reaction (RT-qPCR) assay targeting the NSP3 and VP7 genes of ERVA G3 and G14 genotypes for the rapid detection and G-typing directly from fecal specimens. …”
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Reducering av transportledtid för lövtimmer och brännved genom samtransport med andra sortiment : Reducing transportation lead-times for deciduous saw logs and firewood logs by co-... por Zilo, Torbjörn