Resultados de búsqueda - Sequani~

Molecular epidemiology and spatio-temporal dynamics of the H3N8 equine influenza virus in South America por Olguin Perglione, Cecilia, Golemba, Marcelo D., Torres, Carolina, Barrandeguy, Maria Edith

“…Nucleotide and deduced amino acid sequences for the complete haemagglutinin gene of the H3N8 EIV detected in South America since 1963 were analyzed. …”
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Mycobacterium avium subsp. paratuberculosis (MAP) molecular diversity in cattle, sheep, and goats from Latin America and the Caribbean: a systematic review por Correa-Valencia, Nathalia M., Moyano, Roberto Damian, Hernández-Agudelo, Miguel, Fernández-Silva, Jorge A.

“…The relevant articles reported the use of six different genotyping techniques (i.e., polymerase chain reaction-restriction endonuclease analysis, restriction fragment length polymorphism, type-specific-PCR, mycobacterial interspersed repetitive units-variable number of tandem repeats, multi-locus short sequence repeat, single nucleotide polymorphism) in isolates from seven countries. …”
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Integrating genomic information and productivity and climate-adaptability traits into a regional white spruce breeding program por Cappa, Eduardo Pablo, Klutsch, Jennifer G., Sebastian-Azcona, Jaime, Ratcliffe, Blaise, Xiaojing, Wei, Da Ros, Letitia, Yang, Liu, Chen, Charles, Benowicz, Andy, Sadoway, Shane, Mansfield, Shawn D., Erbilgin, Nadir, Thomas, Barb R., El-Kassaby, Yousry A.

“…We sampled 1,540 trees from three open-pollinated progeny trials, genotyped with 467,224 SNP markers using genotyping-by-sequencing (GBS). We used the pedigree and SNP information to calculate, respectively, the average numerator and genomic relationship matrices, and univariate and multivariate individual-tree models to obtain estimates of (co)variance components. …”
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Projection of soil organic carbon reserves in the Argentine Rolling Pampa under different agronomic scenarios. Relationship of these reserves with some soil properties por Irizar, Alicia Beatriz, Milesi Delaye, Luis Antonio, Andriulo, Adrian Enrique

“…Currently, the soils richest in SOC are cultivated with intensified crop sequences (e.g. maize-double cropped wheat/soybean, MWS) under no tillage (NT) and the poorest ones with soybean monoculture (S) under NT. …”
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Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle por Novoa, María Belén, Valentini, Beatriz Susana, Torioni, Susana Marta, Primo, María Evangelina, Echaide, Ignacio Eduardo

“…A criterion based on the 3-y sequential serologic analysis of 230 dairy cows by IFAT was used as the gold standard. …”
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Characterization of DMI, QoI and SDHI fungicides sensitivity of Ramularia collo‑cygni isolates in Argentina por Erreguerena, Ignacio Antonio, Havis, Neil David, Heick, Thies Marten, Gorniak, Kalina, Quiroz, Facundo Jose, Carmona, Marcelo Anibal

“…For EC50 determination, a sensitivity assay was performed and cytb, cyp51 and sdh genes were sequenced for mutation analyses. All isolates showed G143A mutation that confers total resistance to QoIs (EC50 > 1 mg L−1). …”
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Alfalfa genomic selection for different stress-prone growing regions por Annicchiarico, Paolo, Nazzicari, Nelson, Bouizgaren, Abdelaziz, Hayek, Taoufik, Laouar, Meriem, Cornacchione, Monica, Basigalup, Daniel Horacio, Monterrubio Martin, Cristina, Brummer, Edward Charles, Pecetti, Luciano

“…Our study included 127 genotypes from a Mediterranean reference population originated from three geographically contrasting populations, genotyped via genotyping-by-sequencing and phenotyped based on multi-year biomass dry matter yield of their dense-planted half-sib progenies. …”
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Midgut Genes Knockdown by Oral dsRNA Administration Produces a Lethal Effect on Cotton Boll Weevil por Salvador, Ricardo, Niz, José María, Nakaya, Pablo Angel, Pedarros, Analía Soledad, Hopp, Horacio Esteban

“…Larvae mortality ranged from 40 to 60% depending on the targeted gene sequence. Indeed, α-amylase and cytochrome p450 dsRNAs were the most effective. …”
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Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) por Zhang, Junli, LI, Chengxia, Zhang, Wenjun, Zhang, Xiaoqin, Mo, Youngjun, Tranquilli, Gabriela, Vanzetti, Leonardo Sebastian, Dubcovsky, Jorge

“…This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its over expression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. …”
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A transcriptional analysis of cattle immune cells reveals a central role of type 1 interferon in the In vitro innate immune response against Mycobacterium bovis por Blanco, Federico Carlos, Bigi, María Mercedes, Garcia, Elizabeth Andrea, Elola, María Teresa, Vazquez, Cristina Lourdes, Bigi, Fabiana

“…RNA obtained from the co-cultures was sequenced to identify differentially expressed gene pathways by using the database Reactome. …”
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Arsenic and fluoride in water in northwestern Buenos Aires : their association with natural landscape elements por Alconada Magliano, Margarita Maria, Damiano, Francisco, Carrillo-Rivera, José Joel, Fagundo-Castillo, Juan Reynerio

“…High Arsenic and Fluoride presence in groundwater are explained by discharge flows travelling long distances through a thick sedimentary sequence; relative low concentration is refereed to local travelling flows recharged in nearby high terrain. …”
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Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers por Maroniche, Guillermo Andrés, Sagadin, Monica Beatriz, Mongelli, Vanesa Claudia, Truol, Graciela Ana Maria, Del Vas, Mariana

“…In order to normalize the resulting quantitative data, reference genes with constant expression during the experimental procedures are needed. Results: Partial sequences of the commonly used reference genes actin (ACT), α1-tubulin (TUB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1A), ribosomal protein S18 (RPS18) and polyubiquitin C (UBI) from Delphacodes kuscheli, a planthopper capable of persistently transmitting the plant fijivirus Mal de Río Cuarto virus (MRCV), were isolated for the first time. …”
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CRISPR/Cas9 for potato functional genomics and breeding por Gonzalez, Matías Nicolás, Massa, Gabriela Alejandra, Andersson, Mariette, Storani, Leonardo, Olsson, Niklas, Decima Oneto, Cecilia Andrea, Hofvander, Per, Feingold, Sergio Enrique

“…The establishment of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) as a gene-editing tool has allowed the alteration of specific gene sequences and their concomitant gene function, providing powerful technology for potato gene functional analysis and improvement of elite cultivars. …”
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Assessing small RNA profles in potato diploid hybrid and its resynthesized allopolyploid reveals conserved abundance with distinct genomic distribution por Zavallo, Diego, Cara, Nicolás, Leone, Melisa, Crescente, Juan Manuel, Marfil, Carlos Federico, Masuelli, Ricardo Williams, Asurmendi, Sebastian

“…Small RNAs are key components of the epigenetic mechanisms involved in silencing by RNA-directed DNA Methylation (RdDM). A sRNA sequencing (sRNA-Seq) analysis was performed to individually profile the 21 to 22 nucleotide (21 to 22-nt) and 24-nt sRNA size classes due to their unique mechanism of biogenesis and mode of function. …”
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Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization por Gaset, Braian, Rubio, Gerardo, Ferrari, Manuel Carlos, Cavigliasso, Pablo, Nishinakamasu, Veronica, Puebla, Andrea Fabiana, Faggioli, Valeria Soledad

“…We measured mycorrhizal colonisation to test the plant-AMF relationship and used Illumina MiSeq sequencing of 18S rRNA gene from rhizospheric soil to evaluate AMF diversity. …”
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Comprehensive Analysis of the Influence of Technical and Biological Variations on De Novo Assembly of RNA-Seq Datasets por Gonzalez, Sergio Alberto, Rivarola, Maximo Lisandro, Ribone, Andrés Ignacio, Lew, Sergio Eduardo, Paniego, Norma Beatriz

“…For this purpose, we simulated transcriptomic short-read sequence datasets from high-quality full-length predicted transcript models with varying degrees of complexity. …”
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Molecular analysis of Sarcoptes scabiei infecting wild and domestic South American camelids in Argentina por Anello, Melina, Sosa, Fabiana Evangelina, Ferreyra, Hebe del Valle, Lobo Allende, Rebeca, Mastromatey, Mariana, Uhart, Marcela M., Romero, Sandra Raquel, Florin-Christensen, Monica, Moroni, Barbara, Molinar, Anna Rita, Rossi, Luca, Di Rocco, Florencia

“…Comparison with partial cox1 sequences from other camelids worldwide showed little variation. …”
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Characterization of the SARS-CoV-2 Mutation Pattern Generated In Vitro by the Antiviral Action of Lycorine por Maidana, Silvina Soledad, Romera, Sonia Alejandra, Marandino, Ana, Tau, Rocio Lucia, Shammas, Juan Manuel, Panzera, Yanina, Perez, Ruben

“…Here, we examine the occurrence of single-nucleotide variants (SNVs) and insertions/deletions (indels) in SARS-CoV-2 B.1.499 strain during serial passages in Vero cells, comparing lycorine-treated cultures (2.5 and 5 µg/mL) with untreated controls. Whole-genome sequencing was used to assess mutation patterns and frequencies. …”
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Amblyomma hadanii n. sp. (Acari: Ixodidae), a tick from northwestern Argentina previously confused with Amblyomma coelebs Neumann, 1899 por Nava, Santiago, Mastropaolo, Mariano, Mangold, Atilio Jose, Martins, Thiago F., Venzal, José Manuel, Guglielmone, Alberto

“…Analyses of a 410 bp fragment of the mitochondrial 16S rRNA gene and the complete sequence of the nuclear 18S rRNA gene supported the description of A. hadanii as a new species.…”
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Typification of virulent and low virulence Babesia bigemina clones by 18S rRNA and rap-1c por Thompson, Carolina Soledad, Baravalle, María Eugenia, Valentini, Beatriz Susana, Mangold, Atilio Jose, Torioni, Susana Marta, Ruybal, Paula, Farber, Marisa Diana, Echaide, Ignacio Eduardo

“…The 18S rRNA and rap-1c genes sequences from B. bigemina biological clones and their parental strains, multiplied exclusively in vivo or in vitro, were compared with strain JG-29. …”
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