Resultados de búsqueda - Sequani~

Detection of Four Legume Viruses (AMV, CMV, BYMV, PSbMV) in One Multiplex RT-PCR Test Applied at ICARDA’s Seed Health and Virology Laboratory por Kumari, Safaa, Moukahel, Abdulrahman

“…The Reverse transcription polymerase chain reaction (RT-PCR) is a powerful technique for amplifying specific RNA sequences, enabling the detection of minute quantities of target molecules. …”
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Toward marker-assisted selection in breeding for fusarium wilt tropical race-4 type resistant bananas por Ferreira, C. F., Chen, A., Aitken, E. A. B., Swennen, R., Uwimana, B., de Jesus Rocha, A., da Silva Soares, J. M., de Souza Ramos, A. P., Amorim, E. P.

“…The resistance marker allele was detected in a number of accessions, including improved diploids and commercial cultivars. Sequencing further confirmed the identity of the SNP at this locus. …”
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Superior haplotypes of key drought-responsive genes reveal opportunities for the development of climate-resilient rice varieties por Singh, Preeti, Sundaram, Krishna T., Vinukonda, Vishnu Prasanth, Venkateshwarlu, Challa, Paul, Pronob J., Pahi, Bandana, Gurjar, Anoop, Singh, Uma Maheshwar, Kalia, Sanjay, Kumar, Arvind, Singh, Vikas K., Sinha, Pallavi

“…In this regard, whole-genome re-sequencing of 399 genotypes (landraces and breeding lines) from the 3000 rice genomes panel (3K-RG) is mined to identify the superior haplotypes for 95 drought-responsive candidate genes. …”
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Mapping QTLs and gene validation studies for Mg2+ uptake and translocation using a MAGIC population in rice por Zhi, Shuai, Zou, Wenli, Li, Jinyan, Meng, Lijun, Liu, Jindong, Chen, Jingguang, Ye, Guoyou

“…Analysis of candidate genes in the region of qSMg3 and qTrMg3 through qRT-PCR, complementation assay in the yeast Mg2+ transport-defective mutant CM66, and sequence analysis of the parental lines suggested that LOC_Os03g04360 may play important roles in Mg2+ uptake, translocation and accumulation in rice. …”
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Genetic architecture of the high-inorganic phosphate phenotype derived from a low-phytate mutant in winter wheat por Venegas, Jorge, Guttieri, Mary J., Boehm, Jeffrey D., Graybosch, Robert, Bai, Guihua, St. Amand, Paul Craig, Palmer, Nathan, Hussain, Waseem, Blecha, Sarah, Baenziger, P. Stephen

“…To identify quantitative trait loci (QTL) and develop molecular markers for the HIP phenotype, 171 recombinant inbred lines (RILs) from a winter wheat cross ‘Danby’ × A02568WS‐A‐12‐10 were phenotyped for Pi concentration and genotyped by reduced‐representation sequencing. A total of 1,246 nonredundant, high‐quality single‐nucleotide polymorphisms (SNPs) were used to construct a linkage map spanning 3,272 cM. …”
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Pyricularia sp. jiangsuensis, a new cryptic rice panicle blast pathogen from rice fields in Jiangsu Province, China por Du, Yan, Qi, Zhongqiang, Liang, Dong, Yu, Junjie, Yu, Mina, Zhang, Rongsheng, Cao, Huijuan, Yong, Mingli, Pan, Xiayan, Yin, Xiaole, Qiao, Junqing, Liu, Youzhou, Chen, Zhiyi, Song, Tianqiao, Liu, Wende, Zhang, Zhengguang, Liu, Yongfeng

“…The whole genome of P. sp. jiangsuensis 18‐2 was sequenced. Nine effectors were distributed in translocation or inversion region, which may link to the rapid evolution of effectors. …”
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Development of a genome-wide InDel marker set for allele discrimination between rice (Oryza sativa) and the other seven AA-genome Oryza species por Hechanova, Sherry Lou, Bhattarai, Kamal, Simon, Eliza Vie, Clave, Graciana, Karunarathne, Pathmasiri, Ahn, Eok-Keun, Li, Charng-Pei, Lee, Jeom-Sig, Kohli, Ajay, Hamilton, Ruaraidh Sackville, Hernandez, Jose E., Gregorio, Glenn B., Jena, Kshirod K., An, Gynheung, Kim, Sung-Ryul

“…Here, we developed 541 genome-wide InDel markers for the discrimination of alleles between the cultivated species O. sativa and the other seven AA-genome species by positional multiple sequence alignments among five AA-genome species with four rice varieties. …”
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Marker-assisted introgression and stacking of major QTLs controlling grain number (Gn1a) and number of primary branching (WFP) to NERICA cultivars por Reyes, Vincent P., Angeles-Shim, Rosalyn B., Mendioro, Merlyn S., Manuel, Ma. Carmina C., Lapis, Ruby S., Shim, Junghyun, Sunohara, Hidehiko, Nishiuchi, Shunsaku, Kikuta, Mayumi, Makihara, Daigo, Jena, Kshirod K., Ashikari, Motoyuki, Doi, Kazuyuki

“…Further, genotyping-by-sequencing (GBS) was conducted to validate the introgression and determine the recurrent parent genome recovery (RPGR) of the selected lines. …”
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Molecular programming of drought-challenged Trichoderma harzianum-bioprimed rice (Oryza sativa L.) por Bashyal, Bishnu Maya, Parmar, Pooja, Zaidi, Najam Waris, Aggarwal, Rashmi

“…In the present study, transcriptomic analysis of rice cultivar IR64 bioprimed with Trichoderma harzianum under drought stress was carried out in comparison with drought-stressed samples using next-generation sequencing techniques. Out of the 2,506 significant (p andlt; 0.05) differentially expressed genes (DEGs), 337 (15%) were exclusively expressed in drought-stressed plants, 382 (15%) were expressed in T. harzianum-treated drought-stressed plants, and 1,787 (70%) were commonly expressed. …”
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QTL mapping and favorable allele mining of nitrogen deficiency tolerance using an interconnected breeding population in rice por Shen, Congcong, Chen, Kai, Cui, Yanru, Chen, Jiantao, Mi, Xuefei, Zhu, Shuangbin, Zhu, Yajun, Ali, Jauhar, Ye, Guoyou, Li, Zhikang, Xu, Jianlong

“…Association analysis using 7,388 bins generated by sequencing identified a total of 14, 14, and 12 QTLs for the five traits under low nitrogen (LN), normal nitrogen (NN), and LN/NN conditions, respectively, across three seasons. …”
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Superior haplotypes for haplotype-based breeding for drought tolerance in pigeonpea (Cajanus cajan L.) por Sinha, Pallavi, Singh, Vikas K., Saxena, Rachit K., Khan, Aamir W., Abbai, Ragavendran, Chitikineni, Annapurna, Desai, Aarthi, Molla, Johiruddin, Upadhyaya, Hari D., Kumar, Arvind, Varshney, Rajeev K.

“…In this context, whole genome re‐sequencing data of 292 genotypes from pigeonpea reference set were mined to identify the superior haplotypes for 10 drought‐responsive candidate genes. …”
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A novel locus from the wild allotetraploid rice species Oryza latifolia Desv. confers bacterial blight (Xanthomonas oryzae pv. oryzae) resistance in rice (O. sativa) por Angeles-Shim, Rosalyn B., Shim, Junghyun, Vinarao, Ricky B., Lapis, Ruby S., Singleton, Joshua J.

“…Putative candidate genes that were identified by data mining and comparative sequence analysis can provide targets for further studies on mapping and cloning of the causal gene for PXO339 resistance in the MDILs. …”
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Genome-wide Association Study (GWAS) for mesocotyl elongation in rice (Oryza sativa L.) under multiple culture conditions por Liu, Hongyan, Zhan, Junhui, Li, Jiaolong, Lu, Xiang, Liu, Jindong, Wang, Yamei, Zhao, Quanzhi, Ye, Guoyou

“…A total of 2,338,336 SNPs were discovered by re-sequencing of 208 rice accessions. Genome-wide association study (GWAS) based on mixed linear model (MLM) was conducted and 16 unique loci were identified on chromosomes 1, 2 (2), 3, 4, 5 (2), 6 (2), 7, 8, 9 (2) and 12 (3), respectively, explaining phenotypic variations ranging from 6.3 to 15.9%. …”
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Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines por Horgan, Finbarr G., Srinivasan, Thanga Suja, Crisol-Martínez, Eduardo, Almazan, Maria Liberty P., Ramal, Angelee Fame, Oliva, Ricardo, Quibod, Ian L., Bernal, Carmencita C.

“…After 20 generations on the resistant line, selected leafhoppers attained similar survival, weight gain, and egg laying to leafhoppers that were continually reared on the susceptible recurrent parent, indicating that they had adapted to the resistant host. By sequencing the 16s rRNA gene, we described the microbiome of leafhoppers from colonies associated with five collection sites, and continually reared or switched between NILs. …”
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Tolerance of iron-deficient and -toxic soil conditions in rice por Mahender, Anumalla, Swamy, B.P. Mallikarjuna, Anandan, Annamalai, Ali, Jauhar

“…Therefore, the integration of breeding with advanced genome sequencing and omics technologies allows for the fine-tuning of tolerant genotypes on the basis of molecular genetics, and the further identification of novel genes and transporters that are related to Fe regulation from FTS and FDS conditions is incredibly important to achieve further success in this aspect.…”
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Ancient endogenous pararetroviruses in Oryza genomes provide insights into the heterogeneity of viral gene macroevolution por Chen, Sunlu, Saito, Nozomi, Encabo, Jaymee R., Yamada, Kanae, Choi, Il-Ryong, Kishima, Yuji

“…Endogenous viral sequences in eukaryotic genomes, such as those derived from plant pararetroviruses (PRVs), can serve as genomic fossils to study viral macroevolution. …”
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Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus por Macovei, Anca, Sevilla, Neah R., Cantos, Christian, Jonson, Gilda B., Slamet-Loedin, Inez, Čermák, Tomáš, Voytas, Daniel F., Choi, Il-Ryong, Chadha-Mohanty, Prabhjit

“…The final products with RTSV resistance and enhanced yield under glasshouse conditions were found to no longer contain the Cas9 sequence. Hence, the RTSV‐resistant plants with the novel eIF4G alleles represent a valuable material to develop more diverse RTSV‐resistant varieties.…”
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Yield traits and associated marker segregation in elite introgression lines derived from O. sativa × O. nivara por Haritha, G., Swamy, B.P.M., Naik, M.L., Jyothi, B., Divya, B., Malathi, S., Sarla, N.

“…Segregation of 103 simple sequence repeat (SSR) markers associated with yield-related QTLs was studied. …”
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High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points por Coe, Robert A., Chatterjee, Jolly, Acebron, Kelvin, Dionora, Jacqueline, Mogul, Reychelle, Lin, HsiangChun, Yin, Xiaojia, Bandyopadhyay, Anindya, Sirault, Xavier R. R., Furbank, Robert T., Quick, W. Paul

“…Characterisation and next-generation sequencing of all the mutants identified in this screen may lead to the discovery of novel genes underpinning C4 photosynthesis. …”
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SNP-Seek II: A resource for allele mining and analysis of big genomic data in Oryza sativa por Mansueto, Locedie, Fuentes, Roven Rommel, Chebotarov, Dmytro, Borja, Frances Nikki, Detras, Jeffrey, Abriol-Santos, Juan Miguel, Palis, Kevin, Poliakov, Alexandre, Dubchak, Inna, Solovyev, Victor, Hamilton, Ruaraidh Sackville, McNally, Kenneth L., Alexandrov, Nickolai, Mauleon, Ramil

“…We incorporated legacy phenotypic and passport data for the sequenced varieties originating from the International Rice Genebank Collection Information System (IRGCIS) and gene models from several rice annotation projects. …”
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